SMART-Seq v4 Kit & SMART-Seq v4 PLUS Kit酶试剂盒Takara


SMART-Seq v4 Kit & SMART-Seq v4 PLUS Kit
品牌 Code No. 产品名称 包装量 价格(元) 说明书 数量
Clontech R400752 SMART-Seq® v4 PLUS Kit 48 Rxns ¥47,828 SMART-Seq v4 Kit & SMART-Seq v4 PLUS Kit SMART-Seq v4 Kit & SMART-Seq v4 PLUS Kit SMART-Seq v4 Kit & SMART-Seq v4 PLUS Kit
Clontech R400753 SMART-Seq® v4 PLUS Kit 96 Rxns ¥85,494 SMART-Seq v4 Kit & SMART-Seq v4 PLUS Kit SMART-Seq v4 Kit & SMART-Seq v4 PLUS Kit SMART-Seq v4 Kit & SMART-Seq v4 PLUS Kit
Clontech R400744 Unique Dual Index Kit – 48U 48 Rxns 询价 SMART-Seq v4 Kit & SMART-Seq v4 PLUS Kit SMART-Seq v4 Kit & SMART-Seq v4 PLUS Kit SMART-Seq v4 Kit & SMART-Seq v4 PLUS Kit
Clontech R400745 Unique Dual Index Kit – 96U 96 Rxns 询价 SMART-Seq v4 Kit & SMART-Seq v4 PLUS Kit SMART-Seq v4 Kit & SMART-Seq v4 PLUS Kit SMART-Seq v4 Kit & SMART-Seq v4 PLUS Kit
Clontech 634888 SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing 12 Rxns ¥13,341 SMART-Seq v4 Kit & SMART-Seq v4 PLUS Kit SMART-Seq v4 Kit & SMART-Seq v4 PLUS Kit SMART-Seq v4 Kit & SMART-Seq v4 PLUS Kit
Clontech 634889 SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing 24 Rxns ¥23,046 SMART-Seq v4 Kit & SMART-Seq v4 PLUS Kit SMART-Seq v4 Kit & SMART-Seq v4 PLUS Kit SMART-Seq v4 Kit & SMART-Seq v4 PLUS Kit
Clontech 634890 SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing 48 Rxns ¥37,759 SMART-Seq v4 Kit & SMART-Seq v4 PLUS Kit SMART-Seq v4 Kit & SMART-Seq v4 PLUS Kit SMART-Seq v4 Kit & SMART-Seq v4 PLUS Kit
Clontech 634891 SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing 96 Rxns ¥65,697 SMART-Seq v4 Kit & SMART-Seq v4 PLUS Kit SMART-Seq v4 Kit & SMART-Seq v4 PLUS Kit SMART-Seq v4 Kit & SMART-Seq v4 PLUS Kit
Clontech 634892 SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing 192 Rxns ¥114,330 SMART-Seq v4 Kit & SMART-Seq v4 PLUS Kit SMART-Seq v4 Kit & SMART-Seq v4 PLUS Kit SMART-Seq v4 Kit & SMART-Seq v4 PLUS Kit
Clontech 634893 SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing 480 Rxns ¥228,463 SMART-Seq v4 Kit & SMART-Seq v4 PLUS Kit SMART-Seq v4 Kit & SMART-Seq v4 PLUS Kit SMART-Seq v4 Kit & SMART-Seq v4 PLUS Kit
收藏产品 加入购物车

无标题文档

 
 
SMART-Seq v4 Kit & SMART-Seq v4 PLUS Kit 延伸 SMART-Seq v4 Kit & SMART-Seq v4 PLUS Kit SMART-Seq v4 Kit & SMART-Seq v4 PLUS Kit SMART-Seq v4 Kit & SMART-Seq v4 PLUS Kit
 
  SMART-Seq v4 Kit & SMART-Seq v4 PLUS Kit
 
 
SMART-Seq v4 Kit & SMART-Seq v4 Plus Kit:性能卓越的Ultra low mRNA全长分析
· 高灵敏度 - 可以从10 pg总RNA起始(起始量:1-1000个细胞或10 pg-10 ng总RNA)
· 高质量的RNA-Seq数据 - 保留全长基因信息,低rRNA比率,高基因检出数,有效扩增GC-rich转录本
· 单管操作流程 - SMART-Seq v4 PLUS Kit包含完整的cDNA合成和文库构建流程,分别在单管中进行,减少样品损失与混淆,降低手动操作误差
·增强的测序性能 - SMART-Seq v4 PLUS Kit整合双端特殊index(UDI),即便多个文库pool后用高通量型Illumina测序仪(如Novaseq)分析,也能得到正确结果
·更高的文库产量 - 更便于使用高通量型Illumina测序仪(如Novaseq)测序,或保存剩余的文库用于多次测序
 
产品说明
SMART-Seq v4 Kit(SSv4)和SMART-Seq v4 PLUS Kit是第4代SMARTer Ultra low系统,能正确分析低至10 pg的Ultra low RNA,具有高灵敏度、高再现性的特点。
SMART-Seq v4 Kit是基于经典的SMART技术优化改良而来的,用于Ultra low RNA或少量细胞cDNA合成的试剂盒,提供全长转录本的信息,支持分析转录本异构体、融合基因、点突变等广受科研人员关注的内容,具有更高的灵敏度和再现性。SMART-Seq v4的Oligo中整合锁核酸(Locked Nucleic Acid, LNA)技术,提升了SSv4模板转换效率,能获得更高的再现性、更均一的gene body覆盖度,高GC含量转录本也能得到正确分析。
SMART-Seq v4 PLUS Kit在包含核心的cDNA合成试剂基础上,同时整合了Takara Bio Group专利性的文库构建技术及单次使用的双端特殊index(UDI),提供完整的Ultra low RNA或少量细胞由cDNA合成到文库构建的实验体验。建库试剂中包含片段化酶,以实现对合成cDNA的片段化,接着利用特别的茎环形接头构建高品质Illumina文库。在建库阶段,单管两步操作、过程中无需纯化、2 h即可建成文库!
 
SSv4 Kit:更受客户认可的经典技术!
 
SMART-Seq v4 Kit & SMART-Seq v4 PLUS Kit
图1. SMART-Seq v4 模板转换机制
 
SMART-Seq v4 Kit & SMART-Seq v4 PLUS Kit
表1. 1、100、1000个Hela细胞主要mRNA-Seq数据 (Illumina® MiSeq® platform)
 
SMART-Seq v4 Kit & SMART-Seq v4 PLUS Kit
表2. 10 pg-10 ng的人脑总RNA的mRNA-Seq (Illumina® MiSeq® platform)
 
SSv4 PLUS Kit:不只是一个Kit,更是完整的Ultra low RNA-Seq建库体验
为了使Ultra low RNA或少量细胞cDNA合成、文库构建实验“一步”到位,我们全新推出了SSv4 PLUS Kit,从此您无需再单独购买建库模块,也不用“忍受”不同厂家不同步的供货周期。
 
SMART-Seq v4 Kit & SMART-Seq v4 PLUS Kit
 
SMART-Seq v4 Kit & SMART-Seq v4 PLUS Kit
 
从高灵敏度、高重复性的cDNA合成出发,构建更高品质Illumina文库
Takara科学家从10 pg小鼠脑total RNA起始,采用SMART-Seq v4 Kit完成cDNA合成。随后分别使用SSv4 Plus Kit中的SMART-Seq Library Prep Kit模块与市面上较常用的A公司试剂盒构建文库,并比较性能差异。
 
SMART-Seq v4 Kit & SMART-Seq v4 PLUS Kit
比较数据来源于Takara Bio USA, Inc.
 
从测试数据中可以看出,SSv4 PLUS Kit所建文库的产量高于A公司Kit产量,而更高的文库产量更适于高通量型Illumina测序仪,如Novaseq。
 
两种方法所建文库随后用Nextseq 500完成测序,并对TPM(Transcripts per million)≥0.1的基因数进行统计。SSv4 PLUS文库能比A公司文库检出更多基因,从同样的cDNA起始能“捕获”更全的信息!
 
 
产品详情请点击:SMART-Seq v4 Kit & SMART-Seq v4 PLUS Kit
 
更多实验数据:
SSv4 Kit
SS v4 Plus Kit
 
延伸阅读:
单细胞NGS解决方案
 
 
 

发表回复