CellEase® II DNA快速提取试剂盒 DNA extraction reagent : CellEase® II series


产品编号 产品名称 产品规格 产品等级 产品价格
BIC-BCR10-00001 CellEase® Tissue II 
 DNA提取试剂CellEase ® 组织II
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BIC-BCR10-00002 CellEase®Bacteria II 
 DNA提取试剂CellEase ® 细菌II
见本页
BIC-BCR10-00003 CellEase® Meat II 
 DNA提取试剂CellEase ® 肉II
见本页
BIC-BCR11-00001 CellEase® Blood 
 DNA提取试剂CellEase®血液
见本页
BIC-BCR11-00002 CellEase® Plant 
 DNA提取试剂CellEase ® 植物
见本页
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CellEase® II DNA提取试剂CellEase® II DNA快速提取试剂盒                   DNA extraction reagent : CellEase® II series

DNA extraction reagent : CellEase® II series

CellEase® II DNA快速提取试剂盒                   DNA extraction reagent : CellEase® II series

 

 

 

◆原理

 

此款试剂盒用于细胞内 DNA 提取,操作极为简便,提取的 DNA 可直接用于下游PCR 操作,无需纯化步骤,仅需 9 分钟。CellEase® II 跟其他产品相比无需洗脱,更可提高回收效率。方法简单,只需将样品与 CellEase A和B混合,并进行2步温控孵育即可。

该试剂盒可从动物组织、微生物、植物细胞等样品中制备 DNA。该试剂盒不仅用于常规实验室,也可用在食品检测方面。

 

 

◆优点・特色

 

 ● 快速高效提取DNA

 ● 简单操作即可制备PCR的DNA模板

 ● 成本低

 ● 用于实验室和食品检测

 


DNA 提取试剂 CellEase® II 系列产品

 

 CellEase® II DNA快速提取试剂盒                   DNA extraction reagent : CellEase® II series

 


◆案例・应用


【实验步骤】

 

CellEase® II DNA快速提取试剂盒                   DNA extraction reagent : CellEase® II series

 ● 实验前,请先准备 CellEase® 混合液,按 1:1:3 的比例混合溶液 A、B 和 ddH2O。

 ● 将样品切成合适的大小,放入离心管中。

 ● 向管中加入 CellEase® 混合液。

 ● 72℃下孵育样品 6 分钟。

 ● 接着在 94℃条件下孵育样品 3分钟。

 ● 即可得到纯化 DNA,取适量作为模板,进行 PCR实验。

 


【实验案例】

 

<小鼠尾巴DNA 提取与检测>

CellEase® II DNA快速提取试剂盒                   DNA extraction reagent : CellEase® II series

M :Marker   (100bp ladder)

1 :CellEaseII,   Extracted solution was undiluted

2 :CellEaseII,   Extracted solution was ×10 diluted

3 :CellEaseII,   Extracted solution was ×100 diluted

4 :Conventional   CellEase , Extracted solution was undiluted

5 :Conventional   CellEase , Extracted solution was ×10 diluted

6 :Conventional   CellEase , Extracted solution was ×100 diluted

<全血DNA 提取与检测>

CellEase® II DNA快速提取试剂盒                   DNA extraction reagent : CellEase® II series

Sample: Whole blood (EDTA)

M: Marker (100 bp ladder)

1: CellEase® Blood

2: CellEase® Blood

3: CellEase® Blood

4: Extraction by water


<金黄色葡萄球菌DNA提取与检测>

CellEase® II DNA快速提取试剂盒                   DNA extraction reagent : CellEase® II series

M Marker (500 bp ladder)

1: Undiluted   Sample Add 5 μl of DNA extract to PCR

2: x 10 dilution

3: x 102 dilution

4: x 103 dilution

5: x 104 dilution


6: Undiluted Sample Add 6 μl of DNA extract to PCR

7: x 10 dilution

8: x 102 dilution

9: x 103 dilution

10: x 104 dilution


<番茄叶DNA提取与检测>

CellEase® II DNA快速提取试剂盒                   DNA extraction reagent : CellEase® II series

M: Marker(500 bp ladder)

1: + CellEase®,   Without dilution Add 5 μl to PCR

2: + CellEase®,   ×10 dilution Add 5 μl to PCR

3: + CellEase®,   × 100 dilution Add 5 μl to PCR

4: – CellEase®,   Without dilution Add 5 μl to PCR

5: – CellEase®,   ×10 dilution Add 5 μl to PCR

6: – CellEase®,   × 100 dilution Add 5 μl to PCR

7: + CellEase®,   Without dilution Add 6 μl to PCR

8: + CellEase®,   ×10 dilution Add 6 μl to PCR

9: + CellEase®,   × 100dilution Add 6 μl to PCR


10: – CellEase®,Without dilution Add 6 μl to PCR

11: – CellEase®,×10 dilution Add 6 μl to PCR

12: – CellEase®,×100 dilution Add 6 μl to PCR

13: + CellEase®,Without dilution Add 7 μl to PCR

14: + CellEase®,×10 dilution Add 7 μl to PCR

15: + CellEase®,×100 dilution Add 7 μl to PCR

16: – CellEase®,Without dilution Add 7 μl to PCR

17: – CellEase®,×10 dilution Add 7 μl to PCR

18: – CellEase®,×100 dilution Add 7 μl to PCR

 

<金枪鱼DNA提取与检测>


CellEase® II DNA快速提取试剂盒                   DNA extraction reagent : CellEase® II series

M: Marker(100 bp ladder)

1: Sample 6 mg,   Add 5 μl of DNA extract to PCR

2: Sample 6 mg,   Add 6 μl of DNA extract to PCR

3: Sample 6 mg,   Add 7 μl of DNA extract to PCR

4: Sample 9 mg,   Add 5 μl of DNA extract to PCR

5: Sample 9 mg,   Add 6 μl of DNA extract to PCR

6: Sample 9 mg,   Add 7 μl of DNA extract to PCR

7: Sample 18 mg,  Add 5 μl of DNA extract to PCR

8: Sample 18 mg   Add 6 μl of DNA extract to PCR

9: Sample 18 mg   Add 7 μl of DNA extract to PCR