T5 Exonuclease | NEB酶试剂 New England Biolabs

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T5 Exonuclease

This product is discontinued and has been replaced by T5 Exonuclease (NEB #M0663).

  • Catalog # M0363 was discontinued on October 02, 2020

T5 Exonuclease | NEB酶试剂 New England Biolabs

  • Double-stranded DNA specific exonuclease and single-stranded DNA endonuclease
  • Initiates at the 5′ termini of linear or nicked double-stranded DNA
  • Cleaves linear or nicked double-stranded DNA in the 5′ to 3′ direction

T5 Exonuclease is ideal for:

  • Removal of incomplete ligation products from ligated circular double-stranded DNA
  • Degradation of denatured DNA from alkaline-based plasmid purification methods for improved DNA cloning
  • Degradation of contaminating linear and nicked DNA in plasmid samples 

Storage Temperature: -20°C

T5 Exonuclease | NEB酶试剂 New England Biolabs

产品信息

T5 Exonuclease degrades DNA in the 5´ to 3´ direction (1). T5 Exonuclease is able to initiate nucleotide removal from the 5´ termini or at gaps and nicks of linear or circular dsDNA (1). However, the enzyme does not degrade supercoiled dsDNA (2). T5 Exonuclease also has ssDNA endonuclease activity.

产品来源

An E. coli strain that carries a plasmid with the T5 phage D15 gene.

产品类别:
Discontinued Products

  • 特性和用法

    单位定义

    One unit is defined as the amount of enzyme required to produce 1 nmol of acid soluble deoxyribonucleotide from double-stranded DNA in 30 minutes at 37°C in a total reaction volume of 50 μl.

    反应条件

    1X NEBuffer™ 4
    Incubate at 37°C

    1X NEBuffer™ 4
    50 mM Potassium Acetate
    20 mM Tris-acetate
    10 mM Magnesium Acetate
    1 mM DTT
    (pH 7.9 @ 25°C)

    贮存溶液

    50 mM Tris-HCl
    100 mM NaCl
    1 mM DTT
    0.1 mM EDTA
    50% Glycerol
    0.1% Triton® X-100
    pH 7.5 @ 25°C

    热失活

    否

    单位活性检测条件

    1X NEBuffer 4, 0.15 mM sonicated duplex [3H]-DNA.

  • 优势和特性

    应用特性

    • Degradation of linear ssDNA, dsDNA or nicked plasmid DNA while maintaining supercoiled plasmid DNA.
    • Remove denatured DNA isolated during alkaline lysis-based plasmid purification procedure (5).
    • Enhance the transfection efficiency of miniprep from plasmid cDNA libraries (6).

  • 相关产品

    相关产品

    • Monarch® Plasmid Miniprep Kit 
    • Monarch® DNA Gel Extraction Kit
    • Monarch® PCR & DNA Cleanup Kit (5 μg)

    单独销售的组分

    • NEBuffer 4

  • 参考文献
    1. Sayers, J.R. et al. (1991). Nucleic Acids Res. 19, 4127-4132.
    2. Sayers, J.R. et al. (1990). J. of Biol. Chem. 265, 18311-18317.
    3. Kaliman, A.V. et al. (1986). FEBS. 195, 61-64.
    4. Frenkel, G.D. et al. (1971). J. of Biol. Chem. 246, 4839-4847.
    5. Sayers, J.R. et al. (1996). Analytical Biochem. 241, 186-189.
    6. Kiss-Toth, E. et al. (2001). Analytical Biochem. 288, 230-232.

操作说明、说明书 & 用法

  • 操作说明
    1. Protocol for T5 Exonuclease (M0363)

  • 使用指南
    • Activity of DNA Modifying Enzymes in rCutSmart™ Buffer

工具 & 资源

  • 选择指南
    • Activities of Exonucleases and Non-specific Endonucleases
    • Common Applications for Exonucleases and Endonucleases
    • Properties of Exonucleases and Non-specific Endonucleases

  • Web 工具
    • Exo Selector

  • 研究摘要
    • Choosing the Right Exonuclease (2019)

FAQs & 问题解决指南

  • FAQs
    1. How Does T5 Exonuclease differ from Lambda Exonuclease (NEB# M0262)?
    2. Will T5 exonuclease cleave supercoiled dsDNA?
    3. How does T5 Exonuclease differ from Exonuclease III (NEB# M0206)?
    4. What is the activity of T5 Exonuclease in the NEBuffers?
    5. Can T5 Exonuclease be heat inactivated?
    6. Can DNA be blunted using T5 Exonuclease?
    7. Will T5 Exonuclease work in rCutSmart buffer?

  • 实验技巧

    Has 5’ to 3’ dsDNA exonuclease activity and ssDNA endonuclease activity.

引用 & 技术文献

  • 引用文献

    产品引用文献查找工具

    T5 Exonuclease | NEB酶试剂 New England Biolabs Powered by Bioz See more details on Bioz

  • 专题文章
    • The effect of nucleic acid modifications on digestion by DNA exonucleases

质控、安全 & 法规

  • 质控分析
    每一新批次的 NEB 产品都要经过质控，以满足指定的质量标准，对特定产品的质量标准和每一批次的检测数据可以通过产品说明表格、COA、产品信息卡或者产品手册进行查阅或下载。关于 NEB 产品质控的详细信息，可从此处查阅。

  • 产品说明与变更通知

    产品说明与变更通知

    产品说明表包含产品的储存温度、有效期和规格，这些文件的命名规则如下：[货号]_[规格]_[版本]

    • M0363S_L_v1
    • M0363S_L_v2

  • CoA
    CoA 文件包含单个批次产品的储存温度、有效期和质控，这些文件的命名规则如下： [货号]_[规格]_[版本]_[Lot#]

    • M0363L_v1_10011608
    • M0363L_v1_10024696
    • M0363S_v1_10025500
    • M0363L_v1_10034706
    • M0363S_v1_10039595
    • M0363L_v1_10045738
    • M0363S_v1_10045737
    • M0363L_v1_10055613
    • M0363L_v1_10056173
    • M0363L_v1_10057996
    • M0363S_v1_10057226
    • M0363S_v1_10067121

  • SDS
    以下 SDS 文件可以帮助您安全地使用该产品

    • T5 Exonuclease