BioBrick® Assembly Kit |NEB酶试剂 New England Biolabs

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BioBrick® Assembly Kit

This product was discontinued on 12/15/2021. 

Individual components can be purchased separately. Please contact [email protected].

We are excited to announce that we are in the process of switching all reaction buffers to be BSA-free. Beginning April 2021, we will be gradually transitioning to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. All information on the website has been updated to reflect this change. Find more details at

  • Catalog # E0546 was discontinued on December 15, 2021
  • Assemble multi-compontent genetic systems using BioBrick parts 
  • BioBricks are DNA sequences that encode a defined biological function 
  • Easily assemble any two BioBrick parts using the same protocol

BioBrick® Assembly Kit |


The BioBrick® Assembly Kit provides a streamlined method for assembly of BioBrick parts into multi-component genetic systems. BioBrick parts are DNA sequences that encode a defined biological function and can be readily assembled with any other BioBrick part. The process for assembling any two BioBrick parts is identical and results in a new composite BioBrick part.

The BioBrick Assembly Kit contains EcoRI-HF™, XbaI, SpeI, PstI, T4 DNA Ligase and NEBuffer 2.1.

BioBrick Assembly Overview BioBrick® Assembly Kit |
Discontinued Products

BioBrick® Assembly

  • 特性和用法

操作说明、说明书 & 用法

  • 操作说明
    1. Digestion Protocol for BioBrick Assembly Kit (E0546)
    2. Ligation Protocol using BioBrick Assembly Kit® (E0546)  

FAQs & 问题解决指南

  • FAQs
    1. I am competing in the iGEM competition. Do you have any products that I should consider from NEB?
    2. Can you tell me more about the switch from BSA to Recombinant Albumin (rAlbumin) in NEBuffers?

引用 & 技术文献

  • 引用文献


    BioBrick® Assembly Kit | Powered by Bioz See more details on Bioz

质控、安全 & 法规

  • 质控分析
    每一新批次的 NEB 产品都要经过质控,以满足指定的质量标准,对特定产品的质量标准和每一批次的检测数据可以通过产品说明表格、COA、产品信息卡或者产品手册进行查阅或下载。关于 NEB 产品质控的详细信息,可从此处查阅。

  • CoA
    CoA 文件包含单个批次产品的储存温度、有效期和质控,这些文件的命名规则如下: [货号]_[规格]_[版本]_[Lot#]

    • E0546S_v0_10010950
    • E0546S_v0_10013772
    • E0546S_v0_10023227
    • E0546S_v0_10042692
    • E0546S_v0_10046959
    • E0546S_v0_10053289
    • E0546S_v0_10054220
    • E0546S_v0_10058289
    • E0546S_v0_10116053