T7 DNA Polymerase (unmodified) | NEB酶试剂 New England Biolabs

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T7 DNA Polymerase (unmodified)

T7 DNA Polymerase catalyzes the replication of T7 phage DNA during infection. The high polymerization rate of the enzyme makes long incubations unnecessary.

  • Second strand synthesis in site-directed mutagenesis protocols
  • Gap filling reaction (no strand displacement)
  • T7 DNA Polymerase is not suitable for DNA sequencing
  • Isolated from a recombinant source
  • Supplied with 10X Reaction Buffer

T7 DNA Polymerase (unmodified) | NEB酶试剂 New England Biolabs

库存
货号 浓度 规格 目录价 北京 上海 广州 成都 苏州
M0274S 10,000 units/ml 300 units ¥769.00 有 有 有 无 有
M0274L 10,000 units/ml 1,500 units ¥3,119.00 有 无 无 无 无
如何订购
Please enter a quantity for at least one size

产品信息

T7 DNA Polymerase catalyzes the replication of T7 phage DNA during infection. The protein dimer has two catalytic activities: DNA polymerase activity and strong 3´→ 5´ exonuclease (1,2,3). The high fidelity and rapid extension rate of the enzyme make it particularly useful in copying long stretches of DNA template.

产品来源

T7 DNA Polymerase consists of two subunits: T7 gene 5 protein (84 kilodaltons) and E.coli thioredoxin (12 kilodaltons) (1,4-7). Each protein is cloned and overexpressed in a T7 expression system in E. coli (4).

产品类别:
DNA Manipulation Products

应用:
Isothermal Amplification,
PCR

  • 产品组分信息

    产品组分信息

    本产品提供以下试剂或组分：

    NEB # 名称 组分货号 储存温度 数量 浓度
    • M0274S     -20    
        T7 DNA Polymerase (unmodified) M0274SVIAL -20 1 x 0.03 ml 10,000 units/ml
        T7 DNA Polymerase Reaction Buffer B0274AVIAL -20 1 x 1.5 ml 10 X
        Recombinant Albumin, Molecular Biology Grade B9200SVIAL -20 1 x 0.6 ml 20 mg/ml
    • M0274L     -20    
        T7 DNA Polymerase (unmodified) M0274LVIAL -20 1 x 0.15 ml 10,000 units/ml
        T7 DNA Polymerase Reaction Buffer B0274AVIAL -20 1 x 1.5 ml 10 X
        Recombinant Albumin, Molecular Biology Grade B9200SVIAL -20 1 x 0.6 ml 20 mg/ml

  • 特性和用法

    单位定义

    One unit is defined as the amount of enzyme that will incorporate10 nmoles of dNTP into acid insoluble material in 30 minutes at 37°C .

    反应条件

    1X T7 DNA Polymerase Reaction Buffer
    Supplement with Recombinant Albumin, Molecular Biology Grade
    Incubate at 37°C

    1X T7 DNA Polymerase Reaction Buffer
    20 mM Tris-HCl
    10 mM MgCl2
    1 mM DTT
    (pH 7.5 @ 25°C)

    贮存溶液

    50 mM KPO4
    1 mM DTT
    0.1 mM EDTA
    50% Glycerol
    pH 7 @ 25°C

    热失活

    75°C for 20 min

    5′ – 3′ 核酸外切酶

    No

    3′ – 5′ 核酸外切酶

    Yes

    链置换

    No

    单位活性检测条件

    100 mM KCl, 20 mM Tris-HCl (pH 7.6), 6 mM MgCl2 , 0.5 mM DTT, 0.1 mM EDTA, 50 ug/ml Recombinant Albumin, 150 μM dNTPs including [3H]-dTTP and 70 µg/ml denatured herring sperm DNA.

    错配率

    ~ 15×10-6bases

  • 优势和特性

    应用特性

    • Second strand synthesis in site-directed mutagenesis protocols (8).

  • 相关产品

    相关产品

    • Deoxynucleotide (dNTP) Solution Mix
    • Deoxynucleotide (dNTP) Solution Set
    • Monarch® Plasmid Miniprep Kit 
    • Monarch® DNA Gel Extraction Kit
    • Monarch® PCR & DNA Cleanup Kit (5 μg)

    单独销售的组分

    • 重组白蛋白, 分子生物学级（不含动物成分）

  • 注意事项
    1. The high polymerization rate of the enzyme makes long incubations unnecessary.
    2. T7 DNA Polymerase is not suitable for DNA sequencing.

  • 参考文献
    1. Hori, K. et al. (1979). J. Biol. Chem.. 254, 11598-11604.
    2. Engler, M.J. et al. (1983). J. Biol. Chem.. 258, 11165-11173.
    3. Nordstrom, B. et al. (1981). J. Biol. Chem.. 256, 3112-3117.
    4. Studier, F.W. et al. (1990). Goeddel, D.V.(Ed.), In Methods Enzymol.. 185, 60-89. San Diego: Academic Press.
    5. Grippo, P. and Richardson, C.C. (1971). J. Biol. Chem. 246, 6867-6873.
    6. Modrich, P. and Richardson, C.C. (1975). J. Biol. Chem. 250, 5515-5522.
    7. Adler, S. and Modrich, P. (1979). J. Biol. Chem. 254, 11605-11614.
    8. Bebenek, K. and Kunkel, T.A. (1989). Nucl. Acids Res.. 17, 5408.
    9. Mattila, P., et al. (1991). Nucleic Acids Res.. 19, 4967-4973.

操作说明、说明书 & 用法

  • 使用指南
    • Activity of DNA Modifying Enzymes in rCutSmart™ Buffer

工具 & 资源

  • 选择指南
    • DNA Polymerase Selection Chart

FAQs & 问题解决指南

  • FAQs
    1. Can T7 DNA Polymerase be used in other NEBuffers, including rCutSmart?
    2. Can T7 DNA Polymerase be used to blunt DNA?
    3. Can T7 DNA Polymerase be used to fill in 3′ overhangs?
    4. Can T7 DNA Polymerase be used to remove 5′ overhangs?
    5. Can T7 DNA Polymerase be heat inactivated?
    6. Are deoxynucleotides needed to remove a 3′ overhang using T7 DNA Polymerase?
    7. When should native T7 DNA Polymerase be used?
    8. What is the oligonucleotide-directed mutagenesis without phenotypic selection method?
    9. Can the native T7 DNA Polymerase be used for sequencing?
    10. Does NEB carry modified T7 Polymerase?
    11. Can T7 DNA Polymerase be used in labeling reactions and partial fill in reactions?
    12. Are NEB DNA Polymerases supplied with dNTPs?

引用 & 技术文献

  • 引用文献

    产品引用文献查找工具

    T7 DNA Polymerase (unmodified) | NEB酶试剂 New England Biolabs Powered by Bioz See more details on Bioz

质控、安全 & 法规

  • 质控分析
    每一新批次的 NEB 产品都要经过质控，以满足指定的质量标准，对特定产品的质量标准和每一批次的检测数据可以通过产品说明表格、COA、产品信息卡或者产品手册进行查阅或下载。关于 NEB 产品质控的详细信息，可从此处查阅。

  • 产品说明与变更通知

    产品说明与变更通知

    产品说明表包含产品的储存温度、有效期和规格，这些文件的命名规则如下：[货号]_[规格]_[版本]

    • M0274S_L_v1

  • CoA
    CoA 文件包含单个批次产品的储存温度、有效期和质控，这些文件的命名规则如下： [货号]_[规格]_[版本]_[Lot#]

    • M0274S_L_v1_0111508
    • M0274S_L_v1_0111603
    • M0274S_L_v1_0111609
    • M0274S_L_v1_0111703
    • M0274S_L_v1_0111803
    • M0274L_v1_10012703
    • M0274S_v1_10021765
    • M0274L_v1_10021767
    • M0274L_v1_10039918
    • M0274S_v1_10040917
    • M0274L_v1_10057374
    • M0274S_v1_10059218
    • M0274L_v1_10075649
    • M0274L_v1_10081788
    • M0274S_v1_10091672
    • M0274L_v1_10091094
    • M0274S_v1_10107595
    • M0274S_v1_10114913
    • M0274S_v1_10123097
    • M0274L_v1_10131164
    • M0274S_v1_10135910
    • M0274L_v1_10139934
    • M0274L_v1_10152153
    • M0274S_v1_10161568
    • M0274L_v1_10161623
    • M0274S_v1_10163746

  • SDS
    以下 SDS 文件可以帮助您安全地使用该产品

    • T7 DNA Polymerase (unmodified)
    • T7 DNA Polymerase Reaction Buffer
    • 重组白蛋白, 分子生物学级