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Taq DNA Polymerase with ThermoPol® Buffer
Taq DNA Polymerase is the industry standard for PCR
- Ideal for routine PCR applications
- ThermoPol Buffer delivers high product yield under demanding conditions
- Exceptional value in terms of cost per unit
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Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity (1,2,3) and a 5´ flap endonuclease activity (4,5).
It is supplied with 10X ThermoPol Reaction Buffer, which contains a nonionic detergent to increase enzyme stability during longer incubations.
重点
- Isolated from a recombinant source
- Supplied with 10X Reaction Buffer
- Robust and reliable reactions
- Tolerates a wide range of templates
- Incorporates dUTP, dITP and fluorescently-labeled nucleotides
- Exceptional value in terms of cost per unit
产品来源
An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1
- 产品类别:
- Taq DNA Polymerase Products
- 应用:
- Colony PCR,
- A-tailing,
- Polymerases for DNA Manipulation,
- Multiplex PCR,
- Specialty PCR,
- Routine PCR,
PCR
- 产品组分信息
产品组分信息
本产品提供以下试剂或组分:
NEB # |
名称 |
组分货号 |
储存温度 |
数量 |
浓度 |
-
|
Taq DNA Polymerase with ThermoPol® Buffer |
M0267VVIAL |
-20 |
1 x 0.04 ml |
5,000 units/ml |
|
ThermoPol® Reaction Buffer Pack |
B9004SVIAL |
-20 |
1 x 1.5 ml |
10 X |
|
-
|
Taq DNA Polymerase with ThermoPol® Buffer |
M0267SVIAL |
-20 |
1 x 0.08 ml |
5,000 units/ml |
|
ThermoPol® Reaction Buffer Pack |
B9004SVIAL |
-20 |
2 x 1.5 ml |
10 X |
|
-
|
Taq DNA Polymerase with ThermoPol® Buffer |
M0267LVIAL |
-20 |
1 x 0.4 ml |
5,000 units/ml |
|
ThermoPol® Reaction Buffer Pack |
B9004SVIAL |
-20 |
4 x 1.5 ml |
10 X |
|
-
|
Taq DNA Polymerase with ThermoPol® Buffer |
M0267XVIAL |
-20 |
1 x 0.8 ml |
5,000 units/ml |
|
ThermoPol® Reaction Buffer Pack |
B9004SVIAL |
-20 |
8 x 1.5 ml |
10 X |
|
-
|
Taq DNA Polymerase with ThermoPol® Buffer |
M0267XVIAL |
-20 |
5 x 0.8 ml |
5,000 units/ml |
|
ThermoPol® Reaction Buffer Pack |
B9004SVIAL |
-20 |
40 x 1.5 ml |
10 X |
|
- 特性和用法
单位定义
One unit is defined as the amount of enzyme that will incorporate 15 nmol of dNTP into acid-insoluble material in 30 minutes at 75°C.
反应条件
1X ThermoPol® Reaction Buffer Pack
1X ThermoPol® Reaction Buffer Pack
20 mM Tris-HCl
10 mM (NH4)2SO4
10 mM KCl
2 mM MgSO4
0.1% Triton® X-100
(pH 8.8 @ 25°C)
贮存溶液
10 mM Tris-HCl
100 mM KCl
1 mM DTT
0.1 mM EDTA
0.5% Tween® 20
0.5% IGEPAL® CA-630
50% Glycerol
pH 7.4 @ 25°C
热失活
否
分子量
理论上的: 94000 daltons
5′ – 3′ 核酸外切酶
Yes
3′ – 5′ 核酸外切酶
No
链置换
+
产物末端
单位活性检测条件
1X ThermoPol Reaction Buffer, 200 µM dNTPs including [3H]-dTTP and 15 nM primed M13 DNA.
错配率
~ 285×10-6bases
- 优势和特性
应用特性
- PCR
- Primer extension
- Colony PCR
- 相关产品
相关产品
- Taq 5X Master Mix
- Taq 2X Master Mix
- Taq PCR Kit
- Deoxynucleotide (dNTP) Solution Mix
- Deoxynucleotide (dNTP) Solution Set
- Quick-Load® Taq 2X Master Mix
- Magnesium Sulfate (MgSO4) Solution
单独销售的组分
- ThermoPol® Reaction Buffer Pack
- 注意事项
- 5’→3′ flap endonuclease activity destroys displaced strand.
- 参考文献
- Chien, A., Edgar, D.B. and Trela, J.M. (1976). J. Bact.. 127, 1550-1557.
- Kaledin, A.S., Sliusarenko, A.G. and Gorodetskii, S.I. (1980). Biokhimiya. 45, 644-651.
- Lawyer, F.C. et al. (1993). PCRMethods and Appl.. 2, 275-287.
- Longley, M.J., Bennett, S.E. and Mosbaugh D.W. (1990). NucleicAcids Res.. 18, 7317-7322.
- Lyamichev, V., Brow, M.A. and Dahlberg, J.E. (1993). Science. 260, 778-783.
- Saiki R.K. et al. (1985). Science. 230, 1350-1354.
- Powell, L.M. et al. (1987). Cell. 50, 831-840.
- Sun, Y., Hegamyer, G. and Colburn, N. (1993). Biotechniques. 15, 372-374.
- Sarkar, G., Kapelner, S. and Sommer, S.S. (1990). Nucleic Acids Res.. 18, 7465.
引用 & 技术文献