Taq DNA Polymerase with ThermoPol® Buffer | NEB酶试剂 New England Biolabs

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Taq DNA Polymerase with ThermoPol® Buffer

Taq DNA Polymerase is the industry standard for PCR

  • Ideal for routine PCR applications
  • ThermoPol Buffer delivers high product yield under demanding conditions
  • Exceptional value in terms of cost per unit

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Taq DNA Polymerase with ThermoPol® Buffer | NEB酶试剂 New England Biolabs

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库存
货号 浓度 规格 目录价 北京 上海 广州 成都 苏州
M0267V 5,000 units/ml 200 units ¥349.00
M0267S 5,000 units/ml 400 units ¥659.00
M0267L 5,000 units/ml 2,000 units ¥2,599.00
M0267X 5,000 units/ml 4,000 units ¥4,739.00
M0267E 5,000 units/ml 20,000 units ¥12,799.00
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产品信息

Taq DNA Polymerase with ThermoPol® Buffer | NEB酶试剂 New England Biolabs

Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity (1,2,3) and a 5´ flap endonuclease activity (4,5).

It is supplied with 10X ThermoPol Reaction Buffer, which contains a nonionic detergent to increase enzyme stability during longer incubations.

重点

  • Isolated from a recombinant source
  • Supplied with 10X Reaction Buffer
  • Robust and reliable reactions
  • Tolerates a wide range of templates
  • Incorporates dUTP, dITP and fluorescently-labeled nucleotides
  • Exceptional value in terms of cost per unit

产品来源

An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1

产品类别:
Taq DNA Polymerase Products

应用:
Colony PCR,
A-tailing,
Polymerases for DNA Manipulation,

Multiplex PCR,
Specialty PCR,
Routine PCR,

PCR

  • 产品组分信息

    产品组分信息

    本产品提供以下试剂或组分:

    NEB # 名称 组分货号 储存温度 数量 浓度
    • M0267V     -20    
        Taq DNA Polymerase with ThermoPol® Buffer M0267VVIAL -20 1 x 0.04 ml 5,000 units/ml
        ThermoPol® Reaction Buffer Pack B9004SVIAL -20 1 x 1.5 ml 10 X
    • M0267S     -20    
        Taq DNA Polymerase with ThermoPol® Buffer M0267SVIAL -20 1 x 0.08 ml 5,000 units/ml
        ThermoPol® Reaction Buffer Pack B9004SVIAL -20 2 x 1.5 ml 10 X
    • M0267L     -20    
        Taq DNA Polymerase with ThermoPol® Buffer M0267LVIAL -20 1 x 0.4 ml 5,000 units/ml
        ThermoPol® Reaction Buffer Pack B9004SVIAL -20 4 x 1.5 ml 10 X
    • M0267X     -20    
        Taq DNA Polymerase with ThermoPol® Buffer M0267XVIAL -20 1 x 0.8 ml 5,000 units/ml
        ThermoPol® Reaction Buffer Pack B9004SVIAL -20 8 x 1.5 ml 10 X
    • M0267E     -20    
        Taq DNA Polymerase with ThermoPol® Buffer M0267XVIAL -20 5 x 0.8 ml 5,000 units/ml
        ThermoPol® Reaction Buffer Pack B9004SVIAL -20 40 x 1.5 ml 10 X

  • 特性和用法

    单位定义

    One unit is defined as the amount of enzyme that will incorporate 15 nmol of dNTP into acid-insoluble material in 30 minutes at 75°C.

    反应条件

    1X ThermoPol® Reaction Buffer Pack

    1X ThermoPol® Reaction Buffer Pack
    20 mM Tris-HCl
    10 mM (NH4)2SO4
    10 mM KCl
    2 mM MgSO4
    0.1% Triton® X-100
    (pH 8.8 @ 25°C)

    贮存溶液

    10 mM Tris-HCl
    100 mM KCl
    1 mM DTT
    0.1 mM EDTA
    0.5% Tween® 20
    0.5% IGEPAL® CA-630
    50% Glycerol
    pH 7.4 @ 25°C

    热失活

    分子量

    理论上的: 94000 daltons

    5′ – 3′ 核酸外切酶

    Yes

    3′ – 5′ 核酸外切酶

    No

    链置换

    +

    产物末端

    • Single-base 3´ Overhangs

    单位活性检测条件

    1X ThermoPol Reaction Buffer, 200 µM dNTPs including [3H]-dTTP and 15 nM primed M13 DNA. 

    错配率

    ~ 285×10-6bases

  • 优势和特性

    应用特性

    • PCR
    • Primer extension
    • Colony PCR

  • 相关产品

    相关产品

    • Taq 5X Master Mix
    • Taq 2X Master Mix
    • Taq PCR Kit
    • Deoxynucleotide (dNTP) Solution Mix
    • Deoxynucleotide (dNTP) Solution Set
    • Quick-Load® Taq 2X Master Mix
    • Magnesium Sulfate (MgSO4) Solution

    单独销售的组分

    • ThermoPol® Reaction Buffer Pack

  • 注意事项
    1. 5’→3′ flap endonuclease activity destroys displaced strand.

  • 参考文献
    1. Chien, A., Edgar, D.B. and Trela, J.M. (1976). J. Bact.. 127, 1550-1557.
    2. Kaledin, A.S., Sliusarenko, A.G. and Gorodetskii, S.I. (1980). Biokhimiya. 45, 644-651.
    3. Lawyer, F.C. et al. (1993). PCRMethods and Appl.. 2, 275-287.
    4. Longley, M.J., Bennett, S.E. and Mosbaugh D.W. (1990). NucleicAcids Res.. 18, 7317-7322.
    5. Lyamichev, V., Brow, M.A. and Dahlberg, J.E. (1993). Science. 260, 778-783.
    6. Saiki R.K. et al. (1985). Science. 230, 1350-1354.
    7. Powell, L.M. et al. (1987). Cell. 50, 831-840.
    8. Sun, Y., Hegamyer, G. and Colburn, N. (1993). Biotechniques. 15, 372-374.
    9. Sarkar, G., Kapelner, S. and Sommer, S.S. (1990). Nucleic Acids Res.. 18, 7465.

操作说明、说明书 & 用法

  • 操作说明
    1. PCR Protocol for Taq DNA Polymerase with ThermoPol Buffer (M0267)
    2. A-Tailing with Taq Polymerase

  • 使用指南
    • Activity of Restriction Enzymes in PCR Buffers
    • Guidelines for PCR Optimization with Taq DNA Polymerase
    • Guidelines for PCR Optimization with Thermophilic DNA Polymerases

工具 & 资源

  • 选择指南
    • DNA Polymerase Selection Chart
    • NEB Diluent and Buffer Table
    • Taq Buffer Selection Chart
    • Thermophilic DNA Polymerases

  • Web 工具
    • Tm Calculator

FAQs & 问题解决指南

  • FAQs
    1. Can Taq DNA Polymerase be used in other buffers?
    2. Why is the product a smear when visualized on an agarose gel?
    3. Why is there no product when visualized on an agarose gel?
    4. How should I set up an amplification reaction using Taq DNA Polymerase?
    5. What type of DNA ends result from a primer extension reaction or a PCR using Taq DNA Polymerase?
    6. The product sequence doesn’t completely match the expected sequence. How can this result be improved?
    7. When should Taq DNA Polymerase be used in a primer extension reaction or for PCR?
    8. Will the 5’→3′ flap endonuclease activity of Taq DNA Polymerase degrade primers?
    9. Can Taq DNA Polymerase be used for nick translation?
    10. Does the presence of Ca2+ inhibit PCR?

  • 问题解决指南
    • PCR Troubleshooting Guide
    • Taq PCR Kit Troubleshooting Guide

  • 实验技巧

    Did you know most Taq reactions amplify more efficiently and robustly when you use a 68°C extension temperature?

引用 & 技术文献

  • 引用文献

    产品引用文献查找工具

    Taq DNA Polymerase with ThermoPol® Buffer | NEB酶试剂 New England Biolabs Powered by Bioz See more details on Bioz

质控、安全 & 法规

  • 质控分析
    每一新批次的 NEB 产品都要经过质控,以满足指定的质量标准,对特定产品的质量标准和每一批次的检测数据可以通过产品说明表格、COA、产品信息卡或者产品手册进行查阅或下载。关于 NEB 产品质控的详细信息,可从此处查阅。

  • 产品说明与变更通知

    产品说明与变更通知

    产品说明表包含产品的储存温度、有效期和规格,这些文件的命名规则如下:[货号]_[规格]_[版本]

    • M0267S_L_X_E_v1
    • M0267S_L_X_E_v2

  • CoA
    CoA 文件包含单个批次产品的储存温度、有效期和质控,这些文件的命名规则如下: [货号]_[规格]_[版本]_[Lot#]

    • M0267S_L_X_E_v1_0141506
    • M0267S_L_X_E_v1_0141606
    • M0267S_L_X_E_v1_0141512
    • M0267S_L_X_E_v1_0141612
    • M0267S_L_X_E_v1_0141705
    • M0267S_L_X_E_v1_0141706
    • M0267S_L_X_E_v1_0141712
    • M0267L_v1_10007346
    • M0267E_v1_10015579
    • M0267X_v1_10015580
    • M0267S_v1_10015721
    • M0267X_v1_10019645
    • M0267X_v1_10019973
    • M0267E_v1_10021952
    • M0267L_v1_10015837
    • M0267S_v1_10017230
    • M0267E_v1_10030654
    • M0267E_v1_10032700
    • M0267S_v1_10029614
    • M0267X_v1_10021953
    • M0267S_v1_10033751
    • M0267E_v1_10038750
    • M0267L_v1_10033874
    • M0267X_v1_10033859
    • M0267S_v1_10041711
    • M0267E_v1_10042534
    • M0267X_v1_10042535
    • M0267E_v1_10046984
    • M0267L_v1_10042542
    • M0267X_v1_10046983
    • M0267S_v1_10046021
    • M0267E_v1_10049322
    • M0267L_v1_10049326
    • M0267X_v1_10049713
    • M0267E_v1_10055389
    • M0267L_v1_10055388
    • M0267S_v1_10055386
    • M0267X_v1_10055387
    • M0267X_v1_10055390
    • M0267E_v1_10055895
    • M0267X_v1_10055858
    • M0267E_v1_10060585
    • M0267L_v1_10060582
    • M0267X_v1_10060583
    • M0267E_v1_10062510
    • M0267S_v1_10060586
    • M0267X_v1_10062512
    • M0267E_v1_10063686
    • M0267X_v1_10065824
    • M0267E_v2_10065822
    • M0267S_v2_10065804
    • M0267E_v2_10069930
    • M0267L_v2_10065812
    • M0267L_v2_10069931
    • M0267X_v2_10068685
    • M0267E_v2_10071643
    • M0267S_v2_10069391
    • M0267X_v2_10071642
    • M0267E_v2_10073007
    • M0267X_v2_10073008
    • M0267S_v2_10076985
    • M0267L_v2_10076984
    • M0267X_v2_10079485
    • M0267E_v2_10088155
    • M0267X_v2_10089131
    • M0267E_v2_10091669
    • M0267S_v2_10087105
    • M0267L_v2_10089003
    • M0267E_v2_10093275
    • M0267X_v2_10093274
    • M0267S_v2_10093272
    • M0267X_v2_10096262
    • M0267E_v2_10096267
    • M0267L_v2_10096264
    • M0267X_v2_10100670
    • M0267E_v2_10103380
    • M0267S_v2_10103379
    • M0267E_v2_10106869
    • M0267L_v2_10108515
    • M0267L_v2_10115515
    • M0267S_v2_10108242
    • M0267S_v2_10118262
    • M0267X_v2_10106868
    • M0267X_v2_10112841
    • M0267E_v2_10119759
    • M0267S_v2_10121781
    • M0267X_v2_10114837
    • M0267E_v2_10123931
    • M0267L_v2_10123952
    • M0267X_v2_10123930
    • M0267E_v2_10127972
    • M0267E_v2_10129160
    • M0267L_v2_10129018
    • M0267X_v2_10129159
    • M0267E_v2_10132782
    • M0267S_v2_10127753
    • M0267X_v2_10131905
    • M0267E_v2_10137195
    • M0267X_v2_10137194
    • M0267S_v2_10146110
    • M0267S_v2_10152169
    • M0267S_v2_10160885
    • M0267E_v2_10142678
    • M0267E_v2_10145566
    • M0267E_v2_10148893
    • M0267E_v2_10159334
    • M0267E_v2_10161505
    • M0267L_v2_10147869
    • M0267L_v2_10162099
    • M0267X_v0_10163739
    • M0267X_v2_10142680
    • M0267X_v2_10145437
    • M0267X_v2_10148892
    • M0267X_v2_10154355
    • M0267X_v2_10159335

  • SDS
    以下 SDS 文件可以帮助您安全地使用该产品

    • Taq DNA Polymerase with ThermoPol® Buffer
    • ThermoPol® Reaction Buffer Pack