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WarmStart® Colorimetric LAMP 2X Master Mix with UDG
Simple, one-step solution for Loop-Mediated Isothermal Amplification (LAMP) of DNA or RNA (RT-LAMP) targets
Fast, clear, pink-to-yellow visible detection of amplification
Reduce risk of carryover contamination with thermolabile UDG and dUTP included in the master mix
WarmStart® feature inhibits enzyme activity at room temperature allowing for flexible reaction setup
Learn more about LAMP and other isothermal amplification methods
Learn about a colorimetric RT-LAMP assay used for the detection of Eastern equine encephalitis virus in mosquitoes: AMCA publication
Read how NEB developed and implemented a SARS-CoV-2 RT-LAMP workflow for workplace surveillance: 1) NEB Expressions article 2) PLOS ONE publication
A publication by members of the global LAMP (gLAMP) Consortium provides a comprehensive review of LAMP and its role in the COVID-19 pandemic
Negative reactions are indicated in pink and positive reactions are indicated by a change to yellow.
NEB® LAMP Primer Design Tool Tutorial
WarmStart Colorimetric LAMP 2X Master Mix with UDG is an optimized formulation of Bst 2.0 WarmStart DNA Polymerase and WarmStart RTx in a special low-buffer reaction solution containing a visible pH indicator for rapid and easy detection of LAMP and RT-LAMP reactions. The inclusion of dUTP and UDG in the master mix reduces the possibility of carryover contamination between reactions.
This system is designed to provide a fast, clear visual detection of amplification based on the production of protons and subsequent drop in pH that occurs from the extensive DNA polymerase activity in a LAMP reaction, producing a change in solution color from pink to yellow (an overview of LAMP and primer design can be found in the Featured Videos section). This mix can be used for any LAMP or RT-LAMP reaction and requires only a heated chamber and samples, with readout of positive amplification judged by eye in 15–40 minutes.