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3′-Desthiobiotin-GTP or 3′-Biotin GTP are guanosine triphosphate (GTP) analogs which are modified at their 3’ position with desthiobiotin or biotin, respectively. When used with the Vaccinia Capping System, (NEB #M2080) these reagents enable affinity tagging of RNA triphosphate ends. Tagged RNAs are enriched by binding to Hydrophilic Streptavidin Magnetic Beads (NEB #S1421). Desthiobiotin-tagged RNAs can be eluted with free biotin. This approach is used in Cappable-seq, a method developed at NEB for directly enriching the 5’-ends of primary transcripts (1).