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Exonuclease T
- Also known as RNase T
- Single-stranded (ssDNA or RNA) specific exonuclease
- Catalyzes the removal of nucleotides from linear single-stranded DNA or RNA in the 3′ to 5′ direction
Exonuclease T can be used for:
- Removal of 3′ overhangs of double-stranded DNA to generate blunt-ends*
- Removal of single-stranded primers in PCR reactions prior to DNA sequencing or SNP analysis
- Removal of single-stranded primers for nested PCR reactions
- Removal of single-stranded DNA, leaving behind double-stranded DNA in the sample
*sequence-dependent
