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Vaccinia Capping System
GMP-grade reagent also available. Learn more.
A full system for enzymatic capping based on the Vaccinia virus Capping Enzyme (VCE)
- Adds 7-methylguanylate cap structures (Cap-0) to the 5′ end of RNA generated by in vitro transcription
- Cap-0 is required for efficient translation of the RNA in eukaryotic systems
- mRNA Cap 2´-O-Methyltransferase (NEB #M0366) is required to generate Cap-1 structure that reduces cellular innate immune response when the RNA is used in vivo.
- Isolated from a recombinant source
- Tested for the absence of endonucleases, exonucleases, RNases
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