T4 RNA Ligase 2, truncated |NEB酶试剂 New England Biolabs

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T4 RNA Ligase 2, truncated

  • T4 RNA Ligase 2, truncated (T4 Rnl2tr) specifically ligates the pre-adenylated 5´ end of DNA or RNA to the 3´ end of RNA.
  • The enzyme does not require ATP for ligation but does need the pre-adenylated substrate.
  • T4 Rnl2tr is expressed from a plasmid in E. coli, which encodes the first 249 amino acids of the full length T4 RNA Ligase 2.
  • Unlike the full length ligase, T4 Rnl2tr cannot ligate the phosphorylated 5´ end of RNA or DNA to the 3´ end of RNA.
  • This enzyme, also known as Rnl2 (1–249) has been used for optimized linker ligation for the cloning of microRNAs.
  • This enzyme reduces background ligation because it can only use pre-adenylated linkers.

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T4 RNA Ligase 2, truncated  |

Avoiding RNase Contamination

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M0242S 200,000 units/ml 2,000 units ¥799.00
M0242L 200,000 units/ml 10,000 units ¥3,219.00
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库存 货号 浓度 规格 目录价 北京 上海 广州 成都 苏州 M0242S 200,000 units/ml 2,000 units ¥799.00 有 无 无 无 无 M0242L 200,000 units/ml 10,000 units ¥3,219.00 有 有 无 无 无