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T4 RNA Ligase 1 (ssRNA Ligase), High Concentration
There is also a regular concentration (10,000 units/ml) version of this enzyme available, T4 RNA Ligase 1 (ssRNA Ligase) (NEB #M0204).
T4 RNA Ligase 1 catalyzes the ligation of a 5´ phosphoryl-terminated nucleic acid donor to a 3´ hydroxyl-terminated nucleic acid acceptor through the formation of a 3´ → 5´ phosphodiester bond, with hydrolysis of ATP to AMP and PPi. Substrates include single-stranded RNA and DNA as well as dinucleoside pyrophosphates.
