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pTYB21 is an E. coli expression N-terminal fusion vector designed for in-frame insertion of a target gene into the polylinker downstream of the intein tag. This allows the N-terminus of the target protein to be fused to the intein tag. The self-cleavage activity of the intein allows the release of the target protein from the chitin-bound intein tag, resulting in a single column purification of the target protein.
- View sequence details
- Used in the IMPACT™ Protein Purification System
- Used in conjunction with a C-terminal fusion vector to test which fusion construction (N-terminal or C-terminal) maximizes the expression and yield of a target protein
- 7,514 base pairs in length
- Ampicillin resistant