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NEBExpress® MBP Fusion and Purification System
The NEBExpress® MBP Fusion and Purification System takes advantage of the strong Ptac promoter and the translation initiation signals of maltose binding protein (MBP) to enhance solubility and expression levels of a desired protein in E. coli. The resulting product is an MBP fusion protein, which is then purified by affinity chromatography.
- Reliable E. coli expression: substantial yields (up to 100 mg/L)
- Fusion to MBP has been shown to enhance the solubility of proteins expressed in E. coli (1)
- Two-step purification: amylose elution followed by TEV Protease cleavage and Ni resin isolation results in a highly pure tag-free target protein
- Gentle elution with maltose; no detergents or harsh denaturants required
