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Blue Protein Loading Dye
The blue protein loading dye contains one vial of blue loading buffer and one vial of 30X reducing agent. The combined solution is ideal for protein gel applications.
- 1X Blue Loading Buffer Composition: 62.5 mM Tris-HCl (pH 6.8), 2% (w/v) SDS, 10% glycerol, 0.01% (w/v) bromophenol blue
- 30X Reducing Agent: 1.25 M DTT
- SDS interacts with positively charged amino acids of proteins, thereby disrupting interactions that form protein structures to separate proteins based on size rather than charge or shape
- DTT is present to help reduce structural disulfide bonds
