Luna^® SARS-CoV-2 RT-qPCR Multiplex Assay Kit

The Luna^® SARS-CoV-2 RT-qPCR Multiplex Assay Kit is a real-time RT-PCR assay for the qualitative detection of SARS-CoV-2 nucleic acid. 

• What is the impact of different SARS-CoV-2 variants on this kit, including Omicron? See the updated FAQ and view the Primer Monitor (more info here) for up-to-date information on variants mapped against common primer sets.
• Multiplex detection of 2019-nCoV_N1 and 2019-nCoV_N2 targets and human RNase P gene enables high throughput workflows
• Reduce background amplification from genomic DNA by use of a modified RNase P Internal Control reverse primer to target an exon-exon boundary
• Increase sensitivity with 4X RT-qPCR mix allowing for more sample input
• Luna WarmStart^® RT paired with Hot Start Taq increases reaction specificity and robustness, enabling room temperature setup
• Reduce risk of carryover contamination with thermolabile UDG and dUTP included in the master mix
• Supports sample pooling with minimal loss in sensitivity
• Learn how NEB is supporting COVID-19 research with a variety of RT-qPCR virus detection options

Using the Luna SARS-CoV-2 RT-qPCR Multiplex Assay Kit, up to 94 different samples can be assessed in a single 96-well plate. Anticipated results for each sample type are shown (in each fluorophore channel).