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Taq 5X Master Mix |NEB酶试剂 New England Biolabs
产品信息
Taq 5X Master Mix is an optimized ready-to-use solution containing Taq DNA Polymerase, dNTPs, MgCl2, KCl and stabilizers. It is best for routine PCR applications from templates including pure DNA solutions, bacterial colonies, and cDNA. It can amplify up to 4 kb from complex genomic DNA or up to 5 kb from lambda DNA.
产品来源
An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1.
- 产品类别:
- Taq DNA Polymerase Products,
- Master Mixes Products
- 应用:
- Multiplex PCR,
- Specialty PCR,
- Routine PCR,
PCR
- 产品组分信息
产品组分信息
本产品提供以下试剂或组分：
NEB # 名称 组分货号 储存温度 数量 浓度 -
M0285L -20 Taq 5X Master Mix M0285SVIAL -20 5 x 1 ml 5 X Magnesium Chloride (MgCl2) Solution B9021SVIAL -20 3 x 1.5 ml 25 mM
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- 特性和用法
1X 预混液组分
10 mM Tris-HCl
50 mM KCl
1.5 mM MgCl2
5% Glycerol
0.08% IGEPAL® CA-630
0.05% Tween® 20
0.2 mM dNTPs
25 units/ml Taq DNA Polymerase
pH 8.6 @ 25°C热失活
否
分子量
理论上的: 94000 daltons
5′ – 3′ 核酸外切酶
Yes
3′ – 5′ 核酸外切酶
No
链置换
No
产物末端
- Single-base 3´ Overhangs
单位活性检测条件
1X ThermoPol® Reaction Buffer, 200 µM dNTPs including [3H]-dTTP and 15 nM primed M13 DNA.
Unit Definition:
One unit of Taq DNA Polymerase is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 75°C. - 优势和特性
应用特性
- PCR
- Primer Extension
- Colony PCR
- 相关产品
相关产品
- Taq DNA Polymerase with Standard Taq Buffer
- Deoxynucleotide (dNTP) Solution Set
- Deoxynucleotide (dNTP) Solution Mix
- Standard Taq (Mg-free) Reaction Buffer Pack
- Standard Taq Reaction Buffer Pack
- EpiMark® Methylated DNA Enrichment Kit
单独销售的组分
- Magnesium Chloride (MgCl2) Solution
- 注意事项
- Taq 5X Master Mix is stable for fifteen freeze-thaw cycles when stored at -20°C
- Taq 5X Master Mix is also stable for six months at 4°C, so for frequent use, an aliquot may be kept at 4°C.
- Taq 5X Master Mix should be used at a 1X concentration with DNA template and primers in a total reaction volume of 25 or 50 μl.
- 参考文献
- Chien, A., Edgar, D.B. and Trela, J.M. (1976). J. Bact.. 127, 1550-1557.
- Kaledin, A.S., Sliusarenko, A.G. and Gorodetskii, S.I. (1980). Biokhimiya. 45, 644-651.
- Lawyer, F.C. et al. (1993). PCR Methods and Appl.. 2, 275-287.
- Longley, M.J., Bennett, S.E. and Mosbaugh D.W. (1990). Nucleic Acids Res.. 18, 7317-7322.
- Lyamichev, V., Brow, M.A. and Dahlberg, J.E. (1993). Science. 260, 778-783.
- Saiki R.K. et al. (1985). Science. 230, 1350-1354.
- Powell, L.M. et al. (1987). Cell. 50, 831-840.
- Sun, Y., Hegamyer, G. and Colburn, N. (1993). Biotechniques. 15, 372-374.
- Sarkar, G., Kapelner, S. and Sommer, S.S. (1990). Nucleic Acids Res.. 18, 7465.
操作说明、说明书 & 用法
- 操作说明
- Protocol for Taq 5X Master Mix (M0285)
- 使用指南
- Activity of Restriction Enzymes in PCR Buffers
- Guidelines for PCR Optimization with Taq DNA Polymerase
- Guidelines for PCR Optimization with Thermophilic DNA Polymerases
工具 & 资源
- 选择指南
- DNA Polymerase Selection Chart
- Thermophilic DNA Polymerases
- Web 工具
- Tm Calculator
FAQs & 问题解决指南
- FAQs
- When should Taq DNA Polymerase be used in a primer extension reaction or for PCR?
- Why is the product a smear when visualized on an agarose gel?
- Why is there no product when visualized on an agarose gel?
- The product sequence doesn’t completely match the expected sequence. How can this result be improved?
- Does the presence of Ca2+ inhibit PCR?
- What is the stability of Taq 5X Master Mix?
- 问题解决指南
- PCR Troubleshooting Guide
- Taq PCR Kit Troubleshooting Guide
- 实验技巧
- Did you know most Taq reactions amplify more efficiently and robustly when you use a 68°C extension temperature?
引用 & 技术文献
- 引用文献
产品引用文献查找工具
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质控、安全 & 法规
- 质控分析
每一新批次的 NEB 产品都要经过质控，以满足指定的质量标准，对特定产品的质量标准和每一批次的检测数据可以通过产品说明表格、COA、产品信息卡或者产品手册进行查阅或下载。关于 NEB 产品质控的详细信息，可从此处查阅。
- 产品说明与变更通知
产品说明与变更通知
产品说明表包含产品的储存温度、有效期和规格，这些文件的命名规则如下：[货号]_[规格]_[版本]
- M0285S_L_v1
- CoA
CoA 文件包含单个批次产品的储存温度、有效期和质控，这些文件的命名规则如下： [货号]_[规格]_[版本]_[Lot#]
- M0285S_L_v1_0201702
- M0285S_L_v1_0211708
- M0285S_L_v1_0221803
- M0285L_v1_10014390
- M0285L_v1_10022736
- M0285L_v1_10026025
- M0285L_v1_10029425
- M0285L_v1_10031125
- M0285L_v1_10032689
- M0285L_v1_10033856
- M0285L_v1_10037190
- M0285L_v1_10042502
- M0285L_v1_10047018
- M0285L_v1_10049769
- M0285L_v1_10056700
- M0285L_v1_10060134
- M0285L_v2_10069613
- M0285L_v2_10073569
- M0285L_v2_10079651
- M0285L_v2_10082436
- M0285L_v2_10086684
- M0285L_v2_10088165
- M0285L_v2_10098095
- M0285L_v2_10104382
- M0285L_v2_10107647
- M0285L_v2_10119086
- M0285L_v2_10127912
- M0285L_v2_10135763
- M0285L_v2_10140995
- M0285L_v2_10146685
- M0285L_v2_10157009
- M0285L_v2_10160667
- SDS
以下 SDS 文件可以帮助您安全地使用该产品
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Taq 5X Master Mix
-
Magnesium Chloride (MgCl2) Solution
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