溶菌酶 (≥40KU/mg, 进口)

上海金畔生物科技有限公司提供溶菌酶 (≥40KU/mg, 进口) ,欢迎访问官网了解更多产品信息。

产品编号 D10376
英文名称 Lysozyme from chicken egg white
中文名称 溶菌酶 (≥40KU/mg, 进口)
别    名 Mucopeptide N-acetylmuramoylhydrolase, Muramidase; lyophilized powder, protein ≥90 %, ≥40,000 units/mg protein;   溶解酵素; 溶菌酶 来源于鸡蛋白; 胞壁质酶; 粘肽 N-乙酰胞壁质水解酶;
保存条件 Store at -20℃.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍 CAS号:12650-88-3
分子量:single-chain 14.3kDa
MDL:MFCD00131557
级别/活性:BR,≥40,000 units/mg protein
纯度:≥90% (HPLC)
外观/性状:lyophilized powder
酶活性定义:One unit will produce a ΔA450 of 0.001 per min at pH 6.24 at 25 °C, using a suspension of Micrococcus lysodeikticus as substrate, in a 2.6 mL reaction mixture (1 cm light path).
Biochem/physiol Actions:
Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed in the presence of EDTA that chelates metal ions in the outer bacterial membrane.

The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M).

L6876 (Sigma-Aldrich)