Bovine IgG ELISA说明书

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Catalog #: 0801198

Bovine IgG ELISA

 

Preparation of Bovine IgG Standards

Tube

Number

Concentration of

Bovine IgG

Volume of Bovine IgG

Standard

Volume of

Assay Diluent

1

125 ng/mL

1000 ml

ml

2

62.5 ng/mL

500 ml of #1

500 ml

3

31.25 ng/mL

500 ml of #2

500 ml

4

15.6 ng/mL

500 ml of #3

500 ml

5

7.8 ng/mL

500 ml of #4

500 ml

6

0 ng/mL

ml

500 ml

 

 

 

试验过程

使用前允许所有试剂达到室温。用于制备标准和样品的标号试管。如果不使用整个96井板,则取下剩余的井板。 从板框和放置到重新密封塑料袋与干燥剂。密封袋并存放在2-8°C。

 

步骤1:将每条标签贴在其末端标签上,以确保在检测过程中这些条带与板框分离时的标识。

 

步骤2:将标准1-6的200ml移入重复井.

 

步骤3:每个样品的移液管200 L成重复的威尔斯。

 

第四步:用盖板盖上盖板,在37°C下孵育30分钟。

 

第五步:抽取每口井的内容物,用1x板冲洗缓冲液冲洗4次。清洗时,用1倍平板冲洗缓冲液的300ml注满井,然后抽吸。执行4次填充/吸气循环 s.在后的清洗周期之后,仔细地在吸水性纸巾的垫子上仔细地敲打盘子,*地擦拭盘子。继续,直到没有观察到可见的液滴板洗缓冲液。

 

步骤6:将检测抗体的100m1移入各标准标本中。

 

不要将检测器抗体添加到底物空白井中。

 

步骤7:用盖板盖上盖板,在37°C下孵育30分钟。

 

步骤8:如步骤6所述,用板洗缓冲器冲洗4次。

 

步骤9:将基材的100m1移入每口井,包括基板空白井。

 

步骤10:室温下将盘子孵育30分钟。含有牛IgG的井中会出现蓝色。

 

步骤11:将100m1的止住溶液注入每口井。将发生从蓝色到黄色的颜色变化。

 

步骤12:在15分钟内,用微滴度平板阅读器读取450 nm处每口井的光密度。

 

试样稀释

 

牛初乳通常含有50~80 mg/mL的IgG抗体。1:1,000,000牛初乳稀释在试验稀释剂中被推荐用于初步测试。

 

牛乳通常含有0.5mg/mL的IgG抗体。建议在试验稀释液中稀释1:10,000的牛乳进行初步测试。

 

牛血清通常含有25-30mg/mL的IgG抗体。建议在试验稀释剂中稀释1:500,000牛血清进行初步测试。

 

程序流程图

 

制备试剂稀释剂

吸管标本和标准

37℃孵育30分钟

 

制水板

移液管检测器抗体

37℃孵育30分钟

 

 

制水板

 

移液管底液

室温孵育30分钟

 

 

添加停止溶液并在450 nm处读取

 

 

 

贮存

 

在2-8℃下储存所有试剂盒试剂。

不要冻结。

未使用的微量培养板应保存在带有干燥剂的密封袋中,以使暴露于水分降至低。

储存和处理的所有试剂均为ST 至少可以在包盒标签上打印的过期日期之前。

 

货号

品名

目录价

规格

品牌

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2730

10 ug

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10 ug

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1 ug

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1 ug

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4524

1 ug

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4524

1 ug

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4524

1 ug

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10 ug

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0801482DNA-10UG

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10 ug

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10 ug

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801223

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3842

1 L

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1697

120 ml

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ZeptoBind

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rhIL-2 Recominant Human Interleukin-2

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100 ug

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10x Plate Wash Buffer

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0801114

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Rabbit IgG ELISA

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0801201

SIV p27 Antigen ELISA Kit

49764

480 det.

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480 det.

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0801197

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6689

96 det.

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0801183

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6689

96 det.

ZeptoMetrix

0801182

Human IgG ELISA

6689

96 det.

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0801169

SIV p27 Antigen ELISA Kit

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96 det.

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0801137

HIV-1 p24 Extended Range Kit

1346

kit

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0801116

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96 det.

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0801111

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9341

96 det.

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0801096

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1853

100 det.

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0801180

Mouse IgG ELISA

6689

96 det.

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7001

10 ug

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0801084

Anti-HTLV-I gp46 Clone 67 – 5.5.13.1

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100 ug

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0801119

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4388

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0801014

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4388

100 ug

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0801095

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1 mg

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0801097

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1 mg

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0801025

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100 ug

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0801032CFHI

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6045

1.0 ml

ZeptoMetrix

0801033CFHI

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6045

1.0 ml

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0801113

Anti-HTLV-II p19 Clone 78 – 6.18.07

30732

1 mg

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0801093

Anti-HTLV-II p19 Clone 78 – 6.18.07

4388

100 ug

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0801094

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30732

1 mg

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0801107

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1 mg

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0801108

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1 mg

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0801110

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1 mg

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0801127

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1 mg

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30732

1 mg

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100 ug

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0801086

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100 ug

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0801087

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100 ug

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0801032

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27593

1 mg

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0801033

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31181

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0801216

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1287

50 mg at 1.0 OD

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0801217

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1287

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0801218

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1287

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0801219

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1365

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0801384

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5265

600 ul

ZeptoMetrix

0801500

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9360

10 det.

ZeptoMetrix

0801501

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22854

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0801502

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5343

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0801021

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5343

10 strips

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0801215

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1287

50 mg at 1.0 OD

ZeptoMetrix

0801214

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1287

50 mg at 1.0 OD

ZeptoMetrix

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8775

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7995

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ZeptoMetrix

0801199

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7995

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0805002

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7625

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0805004

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10257

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0801210

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1287

50 mg at 1.0 OD

ZeptoMetrix

0801211

Dinucleotide Standards Formamido Cytidine Dimer

1287

50 mg at 1.0 OD

ZeptoMetrix

0801212

Dinucleotide Standards Formamido Thymine Dimer

1287

50 mg at 1.0 OD

ZeptoMetrix

0801213

Dinucleotide Standards Formamido Adenine Dimer

1287

50 mg at 1.0 OD

ZeptoMetrix

0801022

Zika (MR-766) Strips (10/tube)

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10 strips

ZeptoMetrix

0801085

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100 ug

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1 ug

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7001

10 ug

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10 ug

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4524

1 ug

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30732

1 mg

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0801125

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30732

1 mg

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0801126

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30732

1 mg

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30732

1 mg

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30732

1 mg

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4388

100 ug

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0802014

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30732

1 mg

ZeptoMetrix

0801018

Anti-HTLV-I p24 Clone 46 – 3.24.4

4388

100 ug

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0801082

Anti-HTLV-I p19 Clone 45 – 6.11.1.3

4388

100 ug

ZeptoMetrix

0801123

Anti-HIV-l p17 Clone 32 – 5.8.42

30732

1 mg

ZeptoMetrix

0801122

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30732

1 mg

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0801121

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30732

1 mg

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100 ug

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Anti-HIV-l RT Clone 39 – 4.12.2

4388

100 ug

ZeptoMetrix

0801076

Anti-HIV-l p17 Clone 2D11

4388

100 ug

ZeptoMetrix

0801077

Anti-HIV-l p17 Clone 32 – 1.24.89

4388

100 ug

ZeptoMetrix

0801078

Anti-HIV-l p24 Clone 32 – 5.17.76

4388

100 ug

ZeptoMetrix

0801079

Anti-HIV-l p24 Clone 39 – 5.1.23

4388

100 ug

ZeptoMetrix

0801080

Anti-HIV-l p24 Clone 39 – 5.4A

4388

100 ug

ZeptoMetrix

0801081

Anti-HIV-l p24 Clone 39 – 6.14

4388

100 ug

ZeptoMetrix

0801115

Anti-HIV-l p17 Clone 32 – 1.24.89

30732

1 mg

ZeptoMetrix

 

 

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