biochain K5011610产品说明书


BioChain成立于1994年,总部位于旧金山湾区。

BioChain是高质量加工生物样品产品和分析服务的先进者,用于推进转化医学,伴随诊断和临床产品研发。BioChain的产品可用于DNA和RNA测序,PCR和RT-PCR,基因表达分析,DNA和RNA纯化,蛋白质提取和纯化以及蛋白质表达分析。BioChain的下一代测序一站式服务,从临床样品采集,样品制备,测序到生物信息学分析,将*改变行业。

除了我们*的生物标本和样品制备业务,BioChain正在进入临床应用领域,为癌症和生殖健康提供临床基因组学诊断产品和临床试验服务。BioChain致力于通过持续流程改进计划以竞争力的价格实现高质量的产品和服务。BioChain建立了全面的质量保证计划,以满足客户的满意度,并为我们的内部生产设施保持ISO 9001:2015和ISO 13485:2003认证。

 

 

cfPure® CellFree DNA Extraction Kit  cfure无细胞DNA提取试剂盒是一种基于磁性珠的DNA提取试剂盒,旨在从人血浆和血清中分离循环无细胞DNA(Cfdna)。该工具包有一个快速,简单,和自动 省略友好协议,允许用户快速提取高质量的cfdna,准备用于下游应用,包括实时PCR、数字PCR和下一代测序。 该试剂盒也是一个强有力的工具液体活检研究,如早期发现肿瘤,无创产前检查等。

 

 

Biochain  K5011610

 

cfPure®无细胞DNA提取试剂盒(100 ml)

概观

BioChain的cfPure®无细胞DNA提取试剂盒专为快速有效纯化无循环细胞DNA(cfDNA)而设计。该系统利用二氧化硅涂层的顺磁颗粒净化无细胞[…]

描述

BioChain的cfPure®无细胞DNA提取试剂盒专为快速有效纯化无循环细胞DNA(cfDNA)而设计。该系统利用二氧化硅涂层的顺磁颗粒来纯化无细胞DNA,从小于1 mL到大于10 mL的血清或血浆。开发缓冲液以确保有效回收100bp-500bp的DNA片段,以大限度地回收无细胞DNA。回收的DNA适用于广泛的下游应用,例如亚硫酸氢盐测序,NGS和qPCR。  

应用:

从血浆,血清和尿液中提取高质量的无细胞DNA,用于新一代测序(NGS),亚硫酸氢盐测序,PCR,qPCR,ddPCR和其他要求苛刻的应用

优点和特点:

•用途广泛:  从单管或24至96孔板中提取<1mL至> 10 mL血清或血浆

•自动化友好:  在KingFisher™和液体处理仪器上,提取大约需要50分钟

•时间效率:  手动提取协议只需不到75分钟

•成本效益:  从1 mL血浆中每次提取的竞争价格<$ 3(250 mL试剂盒的定价)

•洗脱体积:  范围为15μL至50μL

 

基于磁珠的快速协议

cfPure®协议快速简便,允许用户在一小时或更短的时间内处理多个样品,使其成为生物标记物筛选的理想选择。BioChain开发了自己的二氧化硅涂层磁珠技术,旨在在分离过程中有效回收低分子量无细胞DNA。除了易于操作和易于自动化之外,如果您正在净化少量等离子体,您可以使用更少的磁珠溶液 – 节省您的资金。成功分析cfDNA的关键是确保有效纯化较小的DNA片段(100-500 bp)。如图1所示,cfPure®无细胞DNA提取试剂盒可有效回收这些重要的小片段。

 

cfPure – 可扩展的无细胞DNA提取试剂盒:

Cfpure旨在成为可扩展的套件(图2)。这意味着您可以仅使用cfDNA提取所需的珠子和试剂的数量。这也意味着每个试剂盒的提取次数取决于您希望从中纯化DNA的样品量。例如,如果您正在使用1mL样品,我们的100mL试剂盒(目录号K5011610)可处理多达100个样品,我们的250 mL试剂盒(目录号K5011610)可处理250个样品。如果您正在使用10mL样品,我们的100mL试剂盒(目录号K5011610)可处理多达10个样品,我们的250 mL无细胞DNA提取试剂盒(目录号K5011625)可处理25个样品。

cfPure®磁珠净化的优点

与用于分离cfDNA的其他技术相比,磁珠提供许多益处。珠子比玻璃纤维过滤器更有效地结合DNA,从而产生更高和更一致的产量。另外,由于不使用过滤器和真空歧管,因此在提取过程中不存在细胞颗粒堵塞这些物品的风险。这种堵塞问题特别涉及富含蛋白质的大体积样品,例如cfDNA研究中常用的血浆。

 

cfDNA由终用户通过NGS验证

cfPure®fCRDNA提取试剂盒提取的cfDNA非常适用于NGS等下游应用。在第三方研究中,使用BioChain的cfPure®(B),竞争对手T(T)和竞争对手Q(Q,基于柱)的cfDNA提取试剂盒从4个供体的10ml人血浆中提取cfDNA。然后为每个样品构建文库,并进行全基因组测序。从数据分析来看,三种提取方法并未显示出显着的性能差异:插入大小(图3),定位率,全基因组覆盖率,替代率和GC偏差(图4)。

cfDNA的可重复和有效回收

我们优化的基于磁珠的协议使用户能够扩展协议,适用于100μl至10 ml的任何样品体积,具有高度可重复的结果和有效的cfDNA回收率(表1和2)。

 

 

自动化友好

cfPure®CellFree DNA提取试剂盒适用于KingFisher™(图5)和液体处理仪器。使用KingFisher™Flex和BioChain的cfPure®无细胞DNA提取试剂盒可在50分钟内提取多达96个1 ml样品,或在1小时内提取24个5 ml样品。

 

biochain 2018年部分产品列表:

 

K5011610

cfPure® Cell Free DNA Extraction Kit (100 ml)

1 Kit (100 ml)

biochain

K5011625

cfPure® Cell Free DNA Extraction Kit (250 ml)

1 Kit (250 ml)

biochain

K5011625MA

cfPure® MAX Cell-Free DNA Extraction Kit

1 Kit (250 ml)

biochain

Z1510001

SeqPure PCR Purification Kit (5 ml)

5 ml

biochain

Z1510002

SeqPure PCR Purification Kit (50 ml)

50 ml

biochain

Z1510003

SeqPure PCR Purification Kit (500 ml)

500 ml

biochain

L5011001

PureSil-Silica Magnetic Beads

1 L

biochain

L5011010

PureSil-Silica Magnetic Beads

10 mL

biochain

Z1322006

AnaPrep Bacterial DNA Extraction Kit (48) For extracting genomic DNA from bacteria

1 kit (48 extractions)

biochain

Z1322002

AnaPrep Blood DNA Extraction Kit 1200 (48) For extracting genomic DNA from mammalian whole blood, peripheral mononuclear cell, or buffy coat. Sample volume range: up to 1 ml

1 kit (48 extractions)

biochain

Z1322001

AnaPrep Blood DNA Extraction Kit 200 (48) For extracting genomic DNA from mammalian whole blood, peripheral mononuclear cell, or buffy coat. Sample volume range: up to 400 µl

1 kit (48 extractions)

biochain

Z1322005

AnaPrep Cultured Cell DNA Extraction Kit (48) For extracting genomic DNA from up to 5×105 cultured cells

1 kit (48 extractions)

biochain

Z1322009

AnaPrep FFPE DNA Extraction Kit (48) For extracting genomic DNA from formalin-fixed paraffin-embedded tissue (FFPE) samples

1 kit (48 extractions)

biochain

Z1322010

AnaPrep Forensic DNA Extraction Kit (48) For extracting genomic DNA from a wide range of forensic and human identity samples

1 kit (48 extractions)

biochain

Z1322007

AnaPrep HPV DNA Extraction Kit for swab samples (48) For extracting HPV DNA from swab samples

1 kit (48 extractions)

biochain

Z1322008

AnaPrep TB DNA Extraction Kit (48) For extracting Mycobacterium tuberculosis DNA from sputum

1 kit (48 extractions)

biochain

Z1322004

AnaPrep Tissue DNA Extraction Kit (48) For extracting genomic DNA from a variety of animal tissues, swab, and blood stains

1 kit (48 extractions)

biochain

Z1322015

AnaPrep Total RNA Extraction Kit

1 kit

biochain

Z1322017

AnaPrep Total RNA Extraction Kit with DNase-Treatment

1 kit

biochain

Z1322003

AnaPrep Viral Nucleic Acid Extraction Kit (48) For extracting viral nucleic acids from plasma

1 kit (48 extractions)

biochain

Z1322011

AnaPrep Viral/Pathogen Nucleic Acids Extraction Kit A

1 kit

biochain

Z1322012

AnaPrep Viral/Pathogen Nucleic Acids Extraction Kit B

1 kit

biochain

T2235090-SB

CancerSeq™ Paraffin Tissue Tumor Block: Colon

1 Block

biochain

T2235152-SB

CancerSeq™ Paraffin Tissue Tumor Block: Lung

1 Block

biochain

T2235086-ST

CancerSeq™ Paraffin Tissue Tumor Slides: Breast

5 Slides

biochain

T2235152-SC

CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung

5 Curls

biochain

T2235152-SC

CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung

5 Curls

biochain

T2235152-SC

CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung

5 Curls

biochain

T2235152-SC

CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung

5 Curls

biochain

T2235152-SC

CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung

5 Curls

biochain

T2235152-SC

CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung

5 Curls

biochain

T2235152-SC

CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung

5 Curls

biochain

T2235152-SC

CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung

5 Curls

biochain

T2235152-SC

CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung

5 Curls

biochain

T2235152-SC

CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung

5 Curls

biochain

T2235152-SC

CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung

5 Curls

biochain

T2235152-SC

CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung

5 Curls

biochain

T2235152-SC

CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung

5 Curls

biochain

T2235152-SC

CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung

5 Curls

biochain

T2235152-SC

CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung

5 Curls

biochain

T2235152-SC

CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung

5 Curls

biochain

T2235152-SC

CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung

5 Curls

biochain

T2235152-SC

CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung

5 Curls

biochain

T2235152-SC

CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung

5 Curls

biochain

T2235152-SC

CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung

5 Curls

biochain

T2235152-SC

CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung

5 Curls

biochain

T2235086-SB

CancerSeq™ Paraffin Tissue Tumor Block: Breast

1 Block

biochain

T2235218A-SB

CancerSeq™ Paraffin Tissue Tumor Block: Skin Melanoma

1 Block

biochain

T2235090-ST

CancerSeq™ Paraffin Tissue Tumor Slides: Colon

5 Slides

biochain

T2235152-ST

CancerSeq™ Paraffin Tissue Tumor Slides: Lung

5 Slides

biochain

T2235218A-ST

CancerSeq™ Paraffin Tissue Tumor Slides: Skin Melanoma

5 Slides

biochain

T2235086-SC

CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast

5 Curls

biochain

T2235086-SC

CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast

5 Curls

biochain

T2235086-SC

CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast

5 Curls

biochain

T2235086-SC

CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast

5 Curls

biochain

T2235086-SC

CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast

5 Curls

biochain

T2235086-SC

CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast

5 Curls

biochain

T2235086-SC

CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast

5 Curls

biochain

T2235086-SC

CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast

5 Curls

biochain

T2235086-SC

CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast

5 Curls

biochain

T2235086-SC

CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast

5 Curls

biochain

T2235086-SC

CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast

5 Curls

biochain

T2235086-SC

CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast

5 Curls

biochain

T2235086-SC

CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast

5 Curls

biochain

T2235086-SC

CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast

5 Curls

biochain

T2235086-SC

CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast

5 Curls

biochain

T2235248-SC

CancerSeq™ Plus Paraffin Tissue Tumor Sections: Stomach

5 Curls

biochain

T2235248-SC

CancerSeq™ Plus Paraffin Tissue Tumor Sections: Stomach

5 Curls

biochain

T2235248-SC

CancerSeq™ Plus Paraffin Tissue Tumor Sections: Stomach

5 Curls

biochain

T2235248-SC

CancerSeq™ Plus Paraffin Tissue Tumor Sections: Stomach

5 Curls

biochain

T2235248-SC

CancerSeq™ Plus Paraffin Tissue Tumor Sections: Stomach

5 Curls

biochain

T2235248-SC

CancerSeq™ Plus Paraffin Tissue Tumor Sections: Stomach

5 Curls

biochain

Z1510001

SeqPure PCR Purification Kit (5 ml)

5 ml

biochain

Z1510002

SeqPure PCR Purification Kit (50 ml)

50 ml

biochain

Z1510003

SeqPure PCR Purification Kit (500 ml)

500 ml

biochain

L1271010

T4 RNA Ligase 1

1000 Units

biochain

L1271050

T4 RNA Ligase 1

5000 Units

biochain

L1271500

T4 RNA Ligase 1

50000 Units

biochain

L1270010

T4 RNA Ligase 2, truncated

1000 Units

biochain

L1270500

T4 RNA Ligase 2, truncated

50000 Units

biochain

KS072012-I

Aligner 1-12

12

biochain

KS072012-II

Aligner 13-24

1 Kit

biochain

KS072012-III

Aligner 25-36

1 Kit

biochain

KS072012-IV

Aligner 37-48

1 Kit

biochain

KS071012-12

Gel Breaker Tubes

12

biochain

KS071012-11

Gel Cutter

12

biochain

KS071012-13

Gel Filter

12

biochain

KS073012-I

RapidSeq High Yield Directional mRNA Sample Prep Kit – With Aligner 1-12

1 Kit

biochain

KS073012-II

RapidSeq High Yield Directional mRNA Sample Prep Kit – With Aligner 13-24

1 Kit

biochain

KS073012-III

RapidSeq High Yield Directional mRNA Sample Prep Kit – With Aligner 25-36

1 Kit

biochain

KS073012-IV

RapidSeq High Yield Directional mRNA Sample Prep Kit – With Aligner 37-48

1 Kit

biochain

KS073012

RapidSeq High Yield Directional mRNA Sample Prep Kit – Without Aligner

1 Kit

biochain

KS073012-T

RapidSeq High Yield Directional mRNA Sample Prep Kit, Trial version enough for 2 rxn. Customer select aligners

1 Kit

biochain

KS074012-I

RapidSeq High Yield Small RNA Sample Prep Kit – With Aligner 1-12

1 Kit

biochain

KS074012-II

RapidSeq High Yield Small RNA Sample Prep Kit – With Aligner 13-24

1 Kit

biochain

KS074012-III

RapidSeq High Yield Small RNA Sample Prep Kit – With Aligner 25-36

1 Kit

biochain

KS074012-IV

RapidSeq High Yield Small RNA Sample Prep Kit – With Aligner 37-48

1 Kit

biochain

KS074012

RapidSeq High Yield Small RNA Sample Prep Kit – Without Aligners

1 Kit

biochain

KS074012-T

RapidSeq High Yield Small RNA Sample Prep Kit, Trial version enough for 2 rxn. Customer select aligners

1 Kit

biochain

K5017100

Blood and Serum DNA Isolation Kit

1 kit

biochain

K5011610

cfPure® Cell Free DNA Extraction Kit (100 ml)

1 Kit (100 ml)

biochain

K5011625

cfPure® Cell Free DNA Extraction Kit (250 ml)

1 Kit (250 ml)

biochain

K5011625MA

cfPure® MAX Cell-Free DNA Extraction Kit

1 Kit (250 ml)

biochain

K5019100

FFPE Tissue DNA Extraction Kit

1 kit

biochain

K5011350

FFPE Tissue DNA Extraction Kit – Column

1 kit

biochain

K5011450

FFPE Tissue DNA Extraction Kit — Magnetic Beads

1 kit

biochain

K5016005

Genomic DNA Extraction Kit

1 kit

biochain

K5011050

Saliva DNA Isolation Kit

1 kit

biochain

K5018100

Serum DNA Isolation Kit

1 kit

biochain

K5011250

Stool DNA Isolation Kit

1 kit

biochain

K5011150

Urine DNA Isolation Kit

1 kit

biochain

Z5060009

xMag-Streptavidin Microparticles

1 ml

biochain

T6234701-1

FDA Standard Frozen Tissue Array – Human Adult Normal

2 slides

biochain

T6234701-2

FDA Standard Frozen Tissue Array – Human Adult Normal

4 slides

biochain

T6334701-1

FDA Standard Frozen Tissue Array – Mouse Normal

2 slides

biochain

T6334701-2

FDA Standard Frozen Tissue Array – Mouse Normal

4 slides

biochain

T6434701-1

FDA Standard Frozen Tissue Array – Rat Normal

2 slides

biochain

T6434701-2

FDA Standard Frozen Tissue Array – Rat Normal

4 slides

biochain

T6235201

Frozen Tissue Array – 24 Different Prostate Tumors and 4 Corresponding Normal Controls

1 slide

biochain

T6235086-1

Frozen Tissue Array – 37 Different Breast Tumors and 3 Corresponding Normal Controls

1 slide

biochain

T6235086-5

Frozen Tissue Array – 37 Different Breast Tumors and 3 Corresponding Normal Controls

5 slides

biochain

T6235090-1

Frozen Tissue Array – 37 Different Colon Tumors and 3 Corresponding Normal Controls

1 slide

biochain

T6235090-5

Frozen Tissue Array – 37 Different Colon Tumors and 3 Corresponding Normal Controls

5 slides

biochain

T6235142-1

Frozen Tissue Array – 37 Different Kidney Tumors and 3 Corresponding Normal Controls

1 slide

biochain

T6235142-5

Frozen Tissue Array – 37 Different Kidney Tumors and 3 Corresponding Normal Controls

5 slides

biochain

T6235183-1

Frozen Tissue Array – 37 Different Ovarian Tumors and 3 Corresponding Normal Controls

1 slide

biochain

T6235183-5

Frozen Tissue Array – 37 Different Ovarian Tumors and 3 Corresponding Normal Controls

5 slides

biochain

T6235152-1

Frozen Tissue Array – 40 Different Lung Tumors and 5 Corresponding Normal Controls

1 slide

biochain

T6235152-5

Frozen Tissue Array – 40 Different Lung Tumors and 5 Corresponding Normal Controls

5 slides

biochain

T6734700-G02-1

Frozen Tissue Array – Dog Female Adult Normal Tissue, Multi-tissue I

1 slide

biochain

T6734700-G02-5

Frozen Tissue Array – Dog Female Adult Normal Tissue, Multi-tissue I

5 slides

biochain

T6734700-G01-1

Frozen Tissue Array – Dog Male Adult Normal Tissue, Multi-tissue I

1 slide

biochain

T6734700-G01-5

Frozen Tissue Array – Dog Male Adult Normal Tissue, Multi-tissue I

5 slides

biochain

T6234700-1

Frozen Tissue Array – Human Adult Normal Tissue, Multi-tissue I

1 slide

biochain

T6234700-5

Frozen Tissue Array – Human Adult Normal Tissue, Multi-tissue I

5 slides

biochain

T6235700-1

Frozen Tissue Array – Human Tumor and Normal Tissue, Multi-tissue I

1 slide

biochain

T6235700-5

Frozen Tissue Array – Human Tumor and Normal Tissue, Multi-tissue I

5 slides

biochain

T6834700-G02-1

Frozen Tissue Array – Rabbit Female Adult Normal Tissue, Multi-tissue I

1 slide

biochain

T6834700-G02-5

Frozen Tissue Array – Rabbit Female Adult Normal Tissue, Multi-tissue I

5 slides

biochain

T6834700-G01-1

Frozen Tissue Array – Rabbit Male Adult Normal Tissue, Multi-tissue I

1 slide

biochain

T6834700-G01-5

Frozen Tissue Array – Rabbit Male Adult Normal Tissue, Multi-tissue I

5 slides

biochain

T6134035-1

Multiple Species Frozen Tissue Array – Brain

1 slide

biochain

T6134035-5

Multiple Species Frozen Tissue Array – Brain

5 slides

biochain

T6134149-1

Multiple Species Frozen Tissue Array – Liver

1 slide

biochain

T6134149-5

Multiple Species Frozen Tissue Array – Liver

5 slides

biochain

 

 

上海金畔生物科技有限公司是实验试剂一站式采购服务商

1:强大的进口辐射能力,血清、抗体、耗材、大部分限制进口品等。

2:产品种类齐全,经营超过700多个品牌,基本涵盖所有生物实验试剂耗材。

3:提供加急服务,货品一般1-2周到货。

4:富有竞争力的价格优势,绝大部分价格有优势。

5:多年积累良好的信誉,大部分客户提供货到付款服务。客户包括清华、北大、交大、复旦、中山等100多所高校,ROCHE,阿斯利康、国药、fisher等药企。

6:我们还是Santa,Advanced Biotechnologies Inc,Athens Research & Technology,bangs,BBInternational,crystalchem,dianova,FD Neurotechnologies,Inc. FormuMax Scientific,Inc, Genebridege, Glycotope Biotechnology GmbH; iduron,Innovative Research of America, Ludger, neuroprobe,omicronbio, Polysciences,prospecbi, QA-BIO,quickzyme,RESEARCH DIETS,INC,sterlitech;sysy,TriLink BioTechnologies,Inc;worthington-biochem,zyagen等几十家国外公司代理。

7:我们还是invitrogen,qiagen,MiraiBioam,sigma;neb,roche,merck, rnd,BD, GE,pierce,BioLegend等*批发,欢迎合作。