Lambda DNA (dam-) | NEB酶试剂 New England Biolabs

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Lambda DNA (dam-)

  • Lambda DNA is a commonly used DNA substrate
  • Isolated from a dam- E.coli strain, this DNA can be digested with restriction endonucleases that are sensitive to dam methylation
  • View sequence details

Lambda DNA (dam-) | NEB酶试剂 New England Biolabs

货号 浓度 规格 目录价 北京 上海 广州 成都 苏州
N3013S 500 µg/ml 250 µg ¥869.00
N3013L 500 µg/ml 1,250 µg ¥3,349.00
Please enter a quantity for at least one size


Duplex DNA is isolated from bacteriophage lambda (cI857ind 1 Sam 7). The bacteriophage is grown in an E. coli host which is deficient in adenine methylase (dam). Therefore, those deoxyadenosine positions which are methylated in a wild type E. coli host are not methylated in the dam host. This DNA gives complete digests with restriction endonucleases that are sensitive to dam methylation. Lambda DNA (dam-) is 48,502 base pairs in length.


The phage is isolated from a heat-inducible E. coli(dam) lambda lysogen. The DNA is isolated from purified phage by phenol extraction and dialyzed against 10 mM Tris-HCl (pH 8.0)and 1 mM EDTA.

DNA Plasmids & Substrates Products

  • 产品组分信息



    NEB # 名称 组分货号 储存温度 数量 浓度
    • N3013S     -20    
        Lambda DNA (dam-) N3013SVIAL -20 1 x 0.5 ml 500 µg/ml
    • N3013L     -20    
        Lambda DNA (dam-) N3013LVIAL -20 2 x 1.25 ml 500 µg/ml

  • 特性和用法


    10 mM Tris-HCl
    1 mM EDTA
    pH 8 @ 25°C

  • 相关产品


    • Lambda DNA
    • Monarch® Plasmid Miniprep Kit 
    • Monarch® DNA Gel Extraction Kit
    • Monarch® PCR & DNA Cleanup Kit (5 μg)

  • 注意事项
    1. The advantage of this DNA is that it gives complete digests with those restriction endonucleases (BclI, MboI, DpnII, etc.) whose recognition sites are partially modified by most E. coli strains.

  • 参考文献
    1. Daniels, D.L. et al. (1983). Appendix II: Complete Annotated Lambda Sequence. R.W. Hendrix, J.W. Roberts, F.W. Stahl and R.A. Weisberg(Ed.), Lambda-II. 519-676. New York: Cold Spring Harbor Laboratory Press.

FAQs & 问题解决指南

  • FAQs
    1. Is the Lambda DNA (N3011) methylated?
    2. What is the DNA sequence of this product?

引用 & 技术文献

  • 引用文献


    Lambda DNA (dam-) | NEB酶试剂 New England Biolabs Powered by Bioz See more details on Bioz

质控、安全 & 法规

  • 质控分析
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  • 产品说明与变更通知



    • N3013S_L_v1

  • CoA
    CoA 文件包含单个批次产品的储存温度、有效期和质控,这些文件的命名规则如下: [货号]_[规格]_[版本]_[Lot#]

    • N3013S_L_v1_0481401
    • N3013S_L_v1_0481407
    • N3013S_L_v1_0481412
    • N3013S_L_v1_0491412
    • N3013S_L_v1_0491505
    • N3013S_L_v1_0491511
    • N3013S_L_v1_0491603
    • N3013S_L_v1_0491608
    • N3013S_L_v1_0491612
    • N3013S_L_v1_0491704
    • N3013S_L_v1_0491705
    • N3013S_L_v1_0491707
    • N3013S_L_v1_0501802
    • N3013S_L_v1_0501804
    • N3013L_v1_10007983
    • N3013L_v1_10019245
    • N3013S_v1_10019247
    • N3013L_v1_10032407
    • N3013L_v1_10035202
    • N3013S_v1_10032410
    • N3013L_v1_10055063
    • N3013S_v1_10058088
    • N3013L_v1_10067002
    • N3013S_v1_10067003
    • N3013L_v2_10071277
    • N3013S_v2_10089320
    • N3013L_v2_10114564
    • N3013L_v2_10115054
    • N3013S_v2_10119138
    • N3013L_v2_10139395
    • N3013S_v2_10140439
    • N3013L_v2_10140441
    • N3013S_v2_10157758

  • SDS
    以下 SDS 文件可以帮助您安全地使用该产品

    • Lambda DNA (dam-)