WarmStart® LAMP Kit (DNA & RNA) | NEB酶试剂 New England Biolabs

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WarmStart® LAMP Kit (DNA & RNA)

  • Simple, one-step solution for Loop-Mediated Isothermal Amplification (LAMP) of DNA or RNA (RT-LAMP) targets
  • Compatible with multiple detection methods
  • WarmStart® technology inhibits activity at room temperature
  • This reagent has been incorporated into Oxford Nanopore’s LamPORE technology, a combination of LAMP with nanopore sequencing, initially implemented to provide a rapid, low-cost and scalable assay for the detection of SARS-CoV-2.
  • Learn more about how RT-LAMP is being used for rapid COVID-19 screening
  • LAMP Fluorescent Dye (NEB #B1700) component available separately


WarmStart® LAMP Kit (DNA & RNA) | NEB酶试剂 New England Biolabs

NEB® LAMP Primer Design Tool Tutorial


货号 浓度 规格 目录价 北京 上海 广州 成都 苏州
E1700S 不适用 100 reactions ¥2,609.00
E1700L 不适用 500 reactions ¥10,419.00
Please enter a quantity for at least one size


WarmStart® LAMP Kit (DNA & RNA) | NEB酶试剂 New England Biolabs

The WarmStart LAMP Kit (DNA & RNA) is designed to provide a simple, one-step solution for Loop-Mediated Isothermal Amplification (LAMP) of DNA or RNA (RT-LAMP) targets. LAMP and RT-LAMP are commonly used isothermal amplification techniques that provides rapid detection of a target nucleic acid using LAMP-specific primers (supplied by the user) and a strand-displacing DNA polymerase. This kit is supplied with the WarmStart LAMP 2X Master Mix, which contains a blend of Bst 2.0 WarmStart DNA Polymerase and WarmStart RTx Reverse Transcriptase in an optimized LAMP buffer solution. Both Bst 2.0 WarmStart DNA Polymerase and WarmStart RTx Reverse Transcriptase have been engineered for improved performance in LAMP and RT-LAMP reactions. A fluorescent dye is also supplied to enable real-time fluorescence measurement of LAMP. The WarmStart LAMP Kit is compatible with multiple detection methods, including turbidity detection, real-time fluorescence detection (when used with LAMP fluorescent dye) and end-point visualization.

The WarmStart LAMP Kit (DNA & RNA) is compatible with multiple detection methods

WarmStart® LAMP Kit (DNA & RNA) | NEB酶试剂 New England Biolabs
The WarmStart LAMP Kit (DNA & RNA) includes fluorescent dye for real-time fluorescence measurement. Alternately, detection can be accomplished by turbidity detection or a variety of endpoint visualization methods.

For carryover contamination prevention, a version containing dUTP and thermolabile UDG is available: WarmStart Fluorescent LAMP/RT-LAMP Kit (with UDG) (NEB #E1708).

NEB’s WarmStart LAMP Kit (DNA & RNA) offers speed and robust sensitivity

WarmStart® LAMP Kit (DNA & RNA) | NEB酶试剂 New England Biolabs
A RNA target (HMBS2) was amplified from Jurkat total RNA using the WarmStart LAMP Kit and OptiGene Master Mix (ISO-001). Reactions were performed at 65°C for 74 minutes on a real-time thermocycler (Bio-Rad CFX96) in triplicate. Time to result is set as the time at which the fluorescence crossed a threshold of 10% of maximal fluorescence. NEB’s WarmStart LAMP Kit resulted in faster and more robust detection as compared to the OptiGene Master Mix.

NEB’s WarmStart LAMP Kit (DNA & RNA) provides fast LAMP/RT-LAMP results

WarmStart® LAMP Kit (DNA & RNA) | NEB酶试剂 New England Biolabs
Experiments were designed to amplify a DNA target (BRCAb) and a RNA target (HMBS2) by LAMP or RT-LAMP, respectively. A 25 μl reaction containing 10 ng HeLa DNA or 5 ng Jurkat total RNA, 1X Master Mix, 1X LAMP primers and fluorescent dye was incubated on a Bio-Rad CFX98 instrument at 65°C for 75 minutes. Lucigen® OmniAmp® RNA & DNA Kit and OptiGene Isothermal Master Mix (ISO-001 ) were run according to manufacturers’ recommendations.

NEB WarmStart LAMP Kit (DNA & RNA) enables specific amplification across multiple species/targets





B. malayi 

E. coli

C. elegans 

BRCAb (human)






CFTR (human)






LymO (Lyme)






BmHhal (B. malayi)






dnaE (E. coli)






Lec10b (C. elegans)






Lec6 (C. elegans)






In each individual reaction, multiple gDNA templates were added along with primers specific to a target in only one species. Reactions were incubated at 65°C for 74 minutes on a thermocycler (Bio-Rad CFX96) in triplicate. Positive results (+) were observed only in the expected species/target combinations, demonstrating high specificity of the WarmStart LAMP reactions.

Isothermal Amplification & Strand Displacement Products

Loop-Mediated Isothermal Amplification,
Isothermal Amplification

  • 试剂盒组成



    NEB # 名称 组分货号 储存温度 数量 浓度
    • E1700S     -20    
        WarmStart LAMP 2X Master Mix M1700SVIAL -20 1 x 1.25 ml 2 X
        LAMP Fluorescent Dye B1700AVIAL -20 1 x 0.05 ml 50 X
    • E1700L     -20    
        WarmStart LAMP 2X Master Mix M1700SVIAL -20 5 x 1.25 ml 2 X
        LAMP Fluorescent Dye B1700SVIAL -20 1 x 0.25 ml 50 X

  • 特性和用法


    • LAMP Primers (we recommend using NEB LAMP Primer Design Tool)
    • Target Nucleic Acid Samples
    • Molecular Biology Grade H2O
    • Heat block, water bath, real-time turbidimeter or thermocycler (with real-time fluorescence measurement if desired) and instrument-appropriate reaction vessels

  • 相关产品


    • LAMP Fluorescent Dye
    • Bst 2.0 WarmStart DNA Polymerase
    • WarmStart® RTx Reverse Transcriptase
    • Isothermal Amplification Buffer Pack
    • Deoxynucleotide (dNTP) Solution Mix
    • Bst 3.0 DNA Polymerase

  • 参考文献
    1. Notomi, T., et al. (2000). Nucleic Acids Res. 28, PubMedID: E63
    2. Nagamine, K., et al. (2001). Clin. Chem. 47, 1742-1743.
    3. Nagamine, K., Hase, T., Notomi, T. (2002). Mol. Cell Probes. 16, 223-229.
    4. Mori, Y., Nagamine, K., Tomita, N., Notomi, T. (2001). Biochem. Biophys. Res. Commun. 289, 150-154.
    5. Mori, Y., Kitao, M., Tomita, N., Notomi, T. (2004). J. Biochem. Biophys. Methods. 59, 145-157.
    6. Tomita, N., Mori, Y., Kanda, H., Notomi, T. (2008). Nat. Protoc. 3, 877-882.
    7. Tanner, N.A. and Evans, T. C. Jr (2014). Curr. Protoc. Mol. Biol. 105, Unit 15.14.
    8. Hsieh et al. (2014). Chem. Comm. 50, 3747-3749.
    9. Tanner, N.A., Zhang, Y. and Evans, T.C. Jr (2015). Biotechniques. 58, 59-68.

操作说明、说明书 & 用法

  • 操作说明
    1. WarmStart LAMP Kit (DNA & RNA) Protocol (E1700)

  • 说明书

    • manualE1700

  • 应用实例
    • Optimized Integration of New England Biolabs® Loop-mediated Isothermal Amplification (LAMP) Reagents with Axxin ISO Instruments

FAQs & 问题解决指南

  • FAQs
    1. What components are included in the WarmStart LAMP Kit (DNA & RNA)?
    2. What is the optimal LAMP/RT-LAMP amplification temperature?
    3. What is the Mg++ concentration in the WarmStart LAMP/RT-LAMP Master Mix?
    4. How do I use the fluorescent dye?
    5. What types of input samples/materials are compatible with the LAMP/RT-LAMP mixes?
    6. How fast should I expect a result?
    7. How do I confirm a positive result?
    8. Can I set up my LAMP/RT-LAMP reactions at room temperature?
    9. Can I use dUTP and UDG for carryover contamination prevention with the WarmStart LAMP Master Mix?
    10. The WarmStart LAMP Master Mix has some precipitation in the tube after thawing, is this normal?
    11. How do I design LAMP Primers?
    12. What is the difference between NEB #E1708: WarmStart Fluorescent LAMP/RT-LAMP Kit (with UDG) and NEB #E1700: WarmStart LAMP Kit (DNA & RNA)?
    13. Amplification occurred in my NTC sample(s) following isothermal incubation. What happened?
    14. What detection methods can be used for LAMP/RT-LAMP experiments?
    15. What are the advantages of using standard LAMP (NEB #E1700, M1708, E1708) over pH-based colorimetric LAMP (NEB #M1800, M1804)?
    16. Is a separate incubation step required for RT-LAMP?
    17. Does NEB have a master mix for LAMP or RT-LAMP reactions?
    18. What is LAMP and RT-LAMP?

引用 & 技术文献

  • 引用文献


    WarmStart® LAMP Kit (DNA & RNA) | NEB酶试剂 New England Biolabs Powered by Bioz See more details on Bioz

  • 专题文章
    • Using aptamers to control enzyme activities: Hot Start Taq and beyond
    • Many Initiatives Turning to RT-LAMP as Alternative to PCR for Rapid COVID-19 Screening Assays

质控、安全 & 法规

  • 质控分析
    每一新批次的 NEB 产品都要经过质控,以满足指定的质量标准,对特定产品的质量标准和每一批次的检测数据可以通过产品说明表格、COA、产品信息卡或者产品手册进行查阅或下载。关于 NEB 产品质控的详细信息,可从此处查阅。

  • 产品说明与变更通知

    Change Notifications

    The M1700 component is now supplied in Sarstedt vials (February 28, 2022).



    • E1700S_L_v3

  • CoA
    CoA 文件包含单个批次产品的储存温度、有效期和质控,这些文件的命名规则如下: [货号]_[规格]_[版本]_[Lot#]

    • E1700S_L_v1_0051803
    • E1700L_v1_10015905
    • E1700S_v1_10015263
    • E1700L_v1_10036139
    • E1700S_v1_10035550
    • E1700S_v1_10037249
    • E1700L_v1_10041509
    • E1700S_v1_10041510
    • E1700L_v2_10045295
    • E1700S_v2_10045298
    • E1700S_v2_10045662
    • E1700L_v2_10048145
    • E1700S_v2_10048144
    • E1700L_v2_10052320
    • E1700L_v2_10056358
    • E1700S_v2_10056359
    • E1700L_v2_10060284
    • E1700S_v2_10061720
    • E1700L_v2_10065742
    • E1700S_v2_10068971
    • E1700L_v2_10068970
    • E1700L_v2_10070696
    • E1700L_v2_10071632
    • E1700L_v2_10073590
    • E1700S_v2_10071582
    • E1700S_v2_10073816
    • E1700L_v2_10074375
    • E1700S_v2_10074377
    • E1700L_v2_10074440
    • E1700L_v2_10074871
    • E1700L_v2_10075479
    • E1700S_v2_10075340
    • E1700S_v2_10076849
    • E1700L_v2_10076848
    • E1700L_v2_10083971
    • E1700S_v2_10082682
    • E1700L_v2_10088160
    • E1700L_v2_10089589
    • E1700L_v2_10091295
    • E1700S_v2_10088965
    • E1700L_v2_10092679
    • E1700L_v2_10094246
    • E1700S_v3_10094015
    • E1700L_v3_10095915
    • E1700L_v3_10099435
    • E1700L_v3_10102697
    • E1700L_v3_10104085
    • E1700S_v3_10103248
    • E1700S_v3_10109758
    • E1700L_v3_10107128
    • E1700S_v3_10110155
    • E1700S_v3_10117355
    • E1700L_v3_10117586
    • E1700S_v3_10127748
    • E1700L_v3_10133020
    • E1700L_v3_10136877
    • E1700S_v3_10145376
    • E1700L_v3_10141226
    • E1700S_v3_10149829
    • E1700S_v3_10137969
    • E1700S_v3_10154965
    • E1700L_v3_10155252
    • E1700S_v3_10160550

  • SDS
    以下 SDS 文件可以帮助您安全地使用该产品

    • WarmStart LAMP 2X Master Mix
    • LAMP Fluorescent Dye