Bsu DNA Polymerase, Large Fragment | NEB酶试剂 New England Biolabs

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Bsu DNA Polymerase, Large Fragment

Optimal performance in a number of applications

  • Strand displacement DNA synthesis
  • Random primer labeling
  • Second strand cDNA synthesis

Bsu DNA Polymerase, Large Fragment | NEB酶试剂 New England Biolabs

库存
货号 浓度 规格 目录价 北京 上海 广州 成都 苏州
M0330S 5,000 units/ml 200 units ¥729.00 无 有 有 有 无
M0330L 5,000 units/ml 1,000 units ¥2,899.00 无 无 有 无 无
如何订购
Please enter a quantity for at least one size

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产品信息

Bsu DNA Polymerase I, Large Fragment retains the 5´→ 3´ polymerase activity of the Bacillus subtilis DNA polymerase I (1), but lacks the 5´→ 3´ exonuclease domain. This large fragment naturally lacks 3´→ 5´ exonuclease activity.

产品来源

An E. coli strain that contains a genetic fusion of the Bacillus subtilis DNA polymerase I gene (starting from codon 297 thus lacking the 5´→ 3´ exonuclease domain), and the gene coding for maltose binding protein (MBP). The fusion protein is purified to near homogeneity and the MBP portion is cleaved off in vitro. The remaining DNA polymerase is purified free of MBP.

产品类别:
DNA Manipulation Products

应用:
Recombinase Polymerase Amplification and SIBA,
Polymerases for DNA Manipulation,
RT-PCR & cDNA Synthesis,

RT-qPCR, RT-PCR and cDNA Synthesis,
Isothermal Amplification,

PCR

  • 产品组分信息

    产品组分信息

    本产品提供以下试剂或组分：

    NEB # 名称 组分货号 储存温度 数量 浓度
    • M0330S     -20    
        Bsu DNA Polymerase, Large Fragment M0330SVIAL -20 1 x 0.04 ml 5,000 units/ml
        NEBuffer™ 2 B7002SVIAL -20 1 x 1.25 ml 10 X
    • M0330L     -20    
        Bsu DNA Polymerase, Large Fragment M0330LVIAL -20 1 x 0.2 ml 5,000 units/ml
        NEBuffer™ 2 B7002SVIAL -20 1 x 1.25 ml 10 X

  • 特性和用法

    单位定义

    One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C.

    反应条件

    1X NEBuffer™ 2
    Incubate at 37°C

    1X NEBuffer™ 2
    50 mM NaCl
    10 mM Tris-HCl
    10 mM MgCl2
    1 mM DTT
    (pH 7.9 @ 25°C)

    热失活

    75°C for 20 min

    5′ – 3′ 核酸外切酶

    No

    3′ – 5′ 核酸外切酶

    No

    链置换

    +

    单位活性检测条件

    1X NEBuffer 2, 33 µM dNTPs including [3H]-dTTP and 70 µg/ml denatured herring sperm DNA.

  • 优势和特性

    应用特性

    • Random primer labeling
    • Second strand cDNA synthesis
    • Single dA tailing
    • Strand displacement DNA synthesis (2)

  • 相关产品

    相关产品

    • Deoxynucleotide (dNTP) Solution Set
    • Deoxynucleotide (dNTP) Solution Mix

    单独销售的组分

    • NEBuffer 2

  • 注意事项
    1. Bsu DNA Polymerase, Large Fragment is not suitable for generating blunt ends because it lacks the 3´→ 5´ exonuclease necessary to remove non-templated 3´ additions.
    2. Bsu DNA Polymerase, Large Fragment retains 50% activity at 25°C and is twice as active as Klenow Fragment (3´→ 5´ exo–) at this temperature.

  • 参考文献
    1. Okazaki, T. et al. (1964). J. Biol.Chem.. 239, 259-268.
    2. Piepenburg, O. et al. (2006). PLOSBiology. 4, 1115-1121.

工具 & 资源

  • 选择指南
    • DNA Polymerase Selection Chart

引用 & 技术文献

  • 引用文献

    产品引用文献查找工具

    Bsu DNA Polymerase, Large Fragment | NEB酶试剂 New England Biolabs Powered by Bioz See more details on Bioz

    更多引用文献

    • Hoser MJ, Mansukoski HK, Morrical SW, Eboigbodin KE (2014) Strand Invasion Based Amplification (SIBA®): A Novel Isothermal DNA Amplification Technology Demonstrating High Specificity and Sensitivity for a Single Molecule of Target Analyte PLoS One; 9(11), e112656. PubMedID: 25419812, DOI: 10.1371/journal.pone.0112656

质控、安全 & 法规

  • 质控分析
    每一新批次的 NEB 产品都要经过质控，以满足指定的质量标准，对特定产品的质量标准和每一批次的检测数据可以通过产品说明表格、COA、产品信息卡或者产品手册进行查阅或下载。关于 NEB 产品质控的详细信息，可从此处查阅。

  • 产品说明与变更通知

    产品说明与变更通知

    产品说明表包含产品的储存温度、有效期和规格，这些文件的命名规则如下：[货号]_[规格]_[版本]

    • M0330S_L_v1
    • M0330S_L_v2

  • CoA
    CoA 文件包含单个批次产品的储存温度、有效期和质控，这些文件的命名规则如下： [货号]_[规格]_[版本]_[Lot#]

    • M0330S_L_v1_0041510
    • M0330S_L_v1_0041512
    • M0330S_L_v1_0041603
    • M0330S_L_v1_0041608
    • M0330S_L_v1_0041703
    • M0330S_L_v1_0041708
    • M0330S_L_v1_0041709
    • M0330S_L_v1_0041803
    • M0330L_v1_10024009
    • M0330S_v1_10032243
    • M0330L_v1_10038937
    • M0330S_v1_10047110
    • M0330L_v1_10056744
    • M0330S_v1_10060275
    • M0330L_v2_10073425
    • M0330S_v2_10074882
    • M0330S_v2_10079279
    • M0330S_v2_10087685
    • M0330L_v2_10098099
    • M0330S_v2_10102440
    • M0330L_v2_10111032
    • M0330L_v2_10130587
    • M0330S_v2_10111033
    • M0330S_v2_10145822
    • M0330L_v2_10145823
    • M0330S_v2_10161572
    • M0330L_v2_10163191

  • SDS
    以下 SDS 文件可以帮助您安全地使用该产品

    • Bsu DNA Polymerase, Large Fragment
    • NEBuffer™ 2