Hemo KlenTaq® | NEB酶试剂 New England Biolabs

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Hemo KlenTaq®

Eliminate DNA extraction and purification

  • Perform PCR directly from whole blood samples
  • Bypass costly DNA extraction and purification steps
  • Amplify from limited quantities of DNA

Hemo KlenTaq® | NEB酶试剂 New England Biolabs

库存
货号 浓度 规格 目录价 北京 上海 广州 成都 苏州
M0332S 不适用 200 reactions ¥1,229.00 有 有 有 无 无
M0332L 不适用 1,000 reactions ¥4,879.00 无 无 无 无 无
如何订购
Please enter a quantity for at least one size

Hemo KlenTaq® | NEB酶试剂 New England Biolabs Interested in glycerol-free or custom formulations? – contact our Custom Solutions Group

Bulk packaging may also be available and requested for large recurring orders.
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产品信息

Hemo KlenTaq is a truncated version of Taq DNA Polymerase, lacking the first 280 amino acids (1). Hemo KlenTaq also contains mutations that make it resistant to inhibitors present in whole blood. It can amplify directly from whole blood from humans and mice. Hemo KlenTaq tolerates up to 20% whole blood in a 25 µl reaction (30% in 50 µl reaction).

Hemo KlenTaq® | NEB酶试剂 New England Biolabs
Amplification of human whole blood with Hemo KlenTaq. Percent blood present in sample and anticoagulant used are indicated in the legend. Ladder M is the 2-Log DNA Ladder (NEB #N3200).

产品来源

An E. coli strain that carries a mutant Taq DNA polymerase gene. The protein lacks the N-terminal 5´→ 3´ exonuclease domain and the gene has three internal point mutations.

产品类别:
Other PCR Polymerases

应用:
Extraction-Free PCR,
Specialty PCR,
PCR

  • 产品组分信息

    产品组分信息

    本产品提供以下试剂或组分：

    NEB # 名称 组分货号 储存温度 数量 浓度
    • M0332S     -20    
        Hemo KlenTaq® M0332SVIAL -20 1 x 0.4 ml 200 reactions
        Hemo KlenTaq® Reaction Buffer Pack B0332SVIAL -20 1 x 1.5 ml 5 X
    • M0332L     -20    
        Hemo KlenTaq® M0332LVIAL -20 2 x 1 ml 500 reactions
        Hemo KlenTaq® Reaction Buffer Pack B0332SVIAL -20 4 x 1.5 ml 5 X

  • 特性和用法

    贮存溶液

    10 mM Tris-HCl
    100 mM KCl
    1 mM DTT
    0.1 mM EDTA
    0.5% Tween® 20
    0.5% IGEPAL® CA-630
    50% Glycerol
    pH 7.4 @ 25°C

    热失活

    否

    5′ – 3′ 核酸外切酶

    No

    3′ – 5′ 核酸外切酶

    No

    产物末端

    • Single-base 3´ Overhangs

    单位活性检测条件

    1X ThermoPol® Reaction Buffer, 200 µM dNTPs including [3H]-dTTP and 15 nM primed M13 DNA.

  • 优势和特性

    Features

    • Versatility and robustness of Taq DNA Polymerase to amplify DNA from whole blood.

    应用特性

    • Whole Blood PCR
    • Dried Blood Spot PCR
    • Primer extension

  • 相关产品

    相关产品

    • Deoxynucleotide (dNTP) Solution Set
    • Deoxynucleotide (dNTP) Solution Mix

    单独销售的组分

    • Hemo KlenTaq® Reaction Buffer Pack

  • 参考文献
    1. Kermekchiev, M.B., et al. (2009). Nucleic Acids Res. 37, e40.
    2. Sun, Y., Hegamyer, G. and Colburn, N. (1993). Biotechniques . 15, 372-374.

操作说明、说明书 & 用法

  • 操作说明
    1. PCR Protocol for Hemo KlenTaq®

FAQs & 问题解决指南

  • 实验技巧
    For best results, slowly pipette your blood sample into the reaction last and let it settle to the bottom of the reaction. The reaction should not be mixed once the blood is added.

引用 & 技术文献

  • 引用文献

    产品引用文献查找工具

    Hemo KlenTaq® | NEB酶试剂 New England Biolabs Powered by Bioz See more details on Bioz

质控、安全 & 法规

  • 质控分析
    每一新批次的 NEB 产品都要经过质控，以满足指定的质量标准，对特定产品的质量标准和每一批次的检测数据可以通过产品说明表格、COA、产品信息卡或者产品手册进行查阅或下载。关于 NEB 产品质控的详细信息，可从此处查阅。

  • 产品说明与变更通知

    产品说明与变更通知

    产品说明表包含产品的储存温度、有效期和规格，这些文件的命名规则如下：[货号]_[规格]_[版本]

    • M0332S_L_v1
    • M0332S_L_v2

  • CoA
    CoA 文件包含单个批次产品的储存温度、有效期和质控，这些文件的命名规则如下： [货号]_[规格]_[版本]_[Lot#]

    • M0332S_L_v1_0151511
    • M0332S_L_v1_0151606
    • M0332S_L_v1_0151612
    • M0332S_L_v1_0151706
    • M0332S_L_v1_0151712
    • M0332S_v1_10014223
    • M0332L_v1_10016580
    • M0332L_v1_10028112
    • M0332L_v1_10032560
    • M0332S_v1_10039476
    • M0332L_v1_10045841
    • M0332S_v1_10048277
    • M0332L_v1_10057553
    • M0332S_v1_10056929
    • M0332S_v2_10069957
    • M0332L_v2_10073431
    • M0332S_v2_10080723
    • M0332L_v2_10080288
    • M0332L_v2_10088805
    • M0332L_v2_10088936
    • M0332L_v2_10098599
    • M0332L_v2_10108251
    • M0332L_v2_10111046
    • M0332L_v2_10135696
    • M0332S_v2_10105148
    • M0332S_v2_10134795
    • M0332L_v2_10140676
    • M0332S_v2_10159481

  • SDS
    以下 SDS 文件可以帮助您安全地使用该产品

    • Hemo KlenTaq®