Quick-Load® Taq 2X Master Mix | NEB酶试剂 New England Biolabs

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Quick-Load® Taq 2X Master Mix

The industry standard

  • Exceptional value in terms of cost per unit
  • 2X master mix format for easy reaction setup – just add template DNA and primers
  • Offered with inert tracking dyes for easy and direct loading of PCR products onto gels

Quick-Load® Taq 2X Master Mix | NEB酶试剂 New England Biolabs

库存
货号 浓度 规格 目录价 北京 上海 广州 成都 苏州
M0271L 2 X 500 reactions (50 µl) ¥2,679.00 无 无 无 无 无
如何订购
Please enter a quantity for at least one size

产品信息

Quick-Load® Taq 2X Master Mix | NEB酶试剂 New England Biolabs  

Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→ 3´ polymerase activity (1,2,3) and a 5´ flap endonuclease activity (4,5). 

Quick-Load® Taq 2X Master Mix is an optimized ready-to-use solution containing Taq DNA Polymerase, dNTPs, MgCl2, KCl, tracking dyes and stabilizers. The presence of two commonly used tracking dyes for DNA gels, Orange G and Xylene Cyanol FF, gives the master mix a green color, allowing direct loading of the PCR product onto agarose gels. On a 1% agarose gel in 1X TBE, Xylene Cyanol FF migrates at approximately 4 kb and Orange G migrates at approximately 50 bp. The amount of tracking dyes included does not mask co-migrating DNA bands. Quick-Load Taq 2X Master Mix is ideally suited to routine PCR applications from templates including pure DNA solutions, bacterial colonies, and cDNA products. It can amplify up to 4 kb from complex genomic DNA or up to 5 kb from lambda DNA.

重点

  • Robust and reliable reactions
  • Tolerates a wide range of templates

产品来源

An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1.

产品类别:
Taq DNA Polymerase Products,
Master Mixes Products

应用:
Polymerases for DNA Manipulation,
Multiplex PCR,
Specialty PCR,

Routine PCR,

PCR

  • 产品组分信息

    产品组分信息

    本产品提供以下试剂或组分：

    NEB # 名称 组分货号 储存温度 数量 浓度
    • M0271L     -20    
        Quick-Load® Taq 2X Master Mix M0271SVIAL -20 10 x 1.25 ml 2 X
        Magnesium Chloride (MgCl2) Solution B9021SVIAL -20 3 x 1.5 ml 25 mM

  • 特性和用法

    1X 预混液组分

    10 mM Tris-HCl
    0.05% Tween® 20
    0.024% Orange G
    0.003% Xylene Cyanol
    33 units/ml Taq DNA Polymerase
    50 mM KCl
    1.5 mM MgCl2
    0.2 mM dATP
    0.2 mM dCTP
    0.2 mM dGTP
    0.2 mM dTTP
    5% Glycerol
    0.08% IGEPAL® CA-630
    pH 8.6 @ 25°C

    热失活

    否

    分子量

    理论上的: 94000 daltons

    5′ – 3′ 核酸外切酶

    Yes

    3′ – 5′ 核酸外切酶

    No

    链置换

    No

    单位活性检测条件

    1X ThermoPol® Reaction Buffer, 200 µM dNTPs including [3H]-dTTP and 15 nM primed M13 DNA.

    Unit Definition:
    One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 75°C.

  • 相关产品

    相关产品

    • Deoxynucleotide (dNTP) Solution Mix
    • Deoxynucleotide (dNTP) Solution Set
    • Standard Taq Reaction Buffer Pack
    • Standard Taq (Mg-free) Reaction Buffer Pack

    单独销售的组分

    • Magnesium Chloride (MgCl2) Solution

  • 注意事项
    1. Keep at -20°C for long term storage. Quick-Load Taq 2X Master Mix is stable for fifteen freeze-thaw cycles when stored at -20°C.
    2. Quick-Load Taq 2X Master Mix is also stable for one week at 4°C, so for daily use, an aliquot may be kept at 4°C.
    3. Quick-Load Taq 2X Master Mix should be used at a 1X concentration with DNA template and primers in a total reaction volume of 25 or 50 μl.

  • 参考文献
    1. Chien, A., Edgar, D.B. and Trela, J.M. (1976). Bact.. 127, 1550-1557.
    2. Kaledin, A.S., Slyusarenko, A.G. and Gorodetskii, S.I. (1980). Biokhimiya. 45, 644-651.
    3. Lawyer, F.C. et al. (1993). PCR Methods and Appl.. 2, 275-287.
    4. Longley, M.J., Bennett, S.E. and Mosbaugh D.W. (1990). Nucleic Acids Res.. 18, 7317-7322.
    5. Lyamichev, V., Brow, M.A. and Dahlberg, J.E. (1993). Science. 260, 778-783.
    6. Saiki R.K. et al. (1985). Science. 230, 1350-1354.
    7. Powell, L.M. et al. (1987). Cell. 50, 831-840.
    8. Sun, Y., Hegamyer, G. and Colburn, N. (1993). Biotechniques. 15, 372-374.
    9. Sarkar, G., Kapelner, S. and Sommer, S.S. (1990). Nucleic Acids Res.. 18, 7465.

操作说明、说明书 & 用法

  • 操作说明
    1. Protocol for Quick-Load® Taq 2X Master Mix

  • 使用指南
    • Activity of Restriction Enzymes in PCR Buffers
    • Guidelines for PCR Optimization with Taq DNA Polymerase
    • Guidelines for PCR Optimization with Thermophilic DNA Polymerases

工具 & 资源

  • 选择指南
    • DNA Polymerase Selection Chart
    • Thermophilic DNA Polymerases

  • Web 工具
    • Tm Calculator

FAQs & 问题解决指南

  • FAQs
    1. When should Taq DNA Polymerase be used in a primer extension reaction or for PCR?
    2. Why is the product a smear when visualized on an agarose gel?
    3. Why is there no product when visualized on an agarose gel?
    4. The product sequence doesn’t completely match the expected sequence. How can this result be improved?
    5. Does the presence of Ca2+ inhibit PCR?
    6. What is the stability of Quick-Load® Taq 2X Master Mix?
    7. What type of DNA ends result from a primer extension reaction or a PCR using Taq DNA Polymerase?
    8. Will the 5’→3′ flap endonuclease activity of Taq DNA Polymerase degrade primers?
    9. Can Taq DNA Polymerase be used for nick translation?

  • 问题解决指南
    • PCR Troubleshooting Guide
    • Taq PCR Kit Troubleshooting Guide

  • 实验技巧
    Did you know most Taq reactions amplify more efficiently and robustly when you use a 68°C extension temperature?

引用 & 技术文献

  • 引用文献

    产品引用文献查找工具

    Quick-Load® Taq 2X Master Mix | NEB酶试剂 New England Biolabs Powered by Bioz See more details on Bioz

质控、安全 & 法规

  • 质控分析
    每一新批次的 NEB 产品都要经过质控，以满足指定的质量标准，对特定产品的质量标准和每一批次的检测数据可以通过产品说明表格、COA、产品信息卡或者产品手册进行查阅或下载。关于 NEB 产品质控的详细信息，可从此处查阅。

  • 产品说明与变更通知

    产品说明与变更通知

    产品说明表包含产品的储存温度、有效期和规格，这些文件的命名规则如下：[货号]_[规格]_[版本]

    • M0271S_L_v1
    • M0271S_L_v2

  • CoA
    CoA 文件包含单个批次产品的储存温度、有效期和质控，这些文件的命名规则如下： [货号]_[规格]_[版本]_[Lot#]

    • M0271S_L_v1_0371706
    • M0271S_L_v1_0371707
    • M0271S_L_v1_0411712
    • M0271L_v1_10015671
    • M0271L_v1_10023170
    • M0271L_v1_10024367
    • M0271L_v1_10030070
    • M0271L_v1_10034024
    • M0271L_v1_10036126
    • M0271L_v1_10042303
    • M0271L_v1_10046051
    • M0271L_v1_10046989
    • M0271L_v1_10053291
    • M0271L_v1_10057158
    • M0271L_v1_10062093
    • M0271L_v1_10065833
    • M0271L_v2_10071654
    • M0271L_v2_10077344
    • M0271L_v2_10080029
    • M0271L_v2_10085961
    • M0271L_v2_10089175
    • M0271L_v2_10097888
    • M0271L_v2_10103969
    • M0271L_v2_10106744
    • M0271L_v2_10111044
    • M0271L_v2_10121789
    • M0271L_v2_10129609
    • M0271L_v2_10132983
    • M0271L_v2_10118534
    • M0271L_v2_10138436
    • M0271L_v2_10140473
    • M0271L_v2_10146693
    • M0271L_v2_10154967
    • M0271L_v2_10161968

  • SDS
    以下 SDS 文件可以帮助您安全地使用该产品

    • Quick-Load® Taq 2X Master Mix
    • Magnesium Chloride (MgCl2) Solution