Hot Start Taq DNA Polymerase |NEB酶试剂 New England Biolabs

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Hot Start Taq DNA Polymerase

The industry standard with hot start performance

  • Ideal for routine PCR applications and molecular diagnostics
  • High specificity and sensitivity
  • Aptamer-based hot start technology avoids nonspecific amplification and enables room temperature reaction setup

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Hot Start Taq DNA Polymerase |

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库存
货号 浓度 规格 目录价 北京 上海 广州 成都 苏州
M0495S 5,000 units/ml 200 units ¥709.00 有 有 无 无 无
M0495L 5,000 units/ml 1,000 units ¥2,809.00 无 有 无 无 有
如何订购
Please enter a quantity for at least one size

Hot Start Taq DNA Polymerase | Interested in glycerol-free or custom formulations? – contact our Custom Solutions Group

Bulk packaging may also be available and requested for large recurring orders.
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产品信息

Hot Start Taq DNA Polymerase |

Hot Start Taq DNA Polymerase is a mixture of Taq DNA Polymerase and an aptamer-based inhibitor. The inhibitor binds reversibly to the enzyme, inhibiting polymerase activity at temperatures below 45°C, but releases the enzyme during normal cycling conditions, allowing reactions to be set up at room temperature. This aptamer-based hot start does not require a separate high temperature incubation step to activate the enzyme. Taq DNA polymerase possesses a 5´→3´ polymerase activity (1)(2)(3)and a 5´ flap endonuclease activity(4)(5).

It is supplied with 10X Standard Taq Reaction Buffer, which is detergent-free and designed to be compatible with existing assay systems.

产品来源

An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1.

产品类别:
Taq DNA Polymerase Products

应用:
Colony PCR,
Multiplex PCR,
Specialty PCR,

Routine PCR,

PCR

  • 产品组分信息

    产品组分信息

    本产品提供以下试剂或组分：

    NEB # 名称 组分货号 储存温度 数量 浓度
    • M0495S     -20    
        Hot Start Taq DNA Polymerase M0495SVIAL -20 1 x 0.04 ml 5,000 units/ml
        Standard Taq Reaction Buffer Pack B9014SVIAL -20 1 x 1.5 ml 10 X
    • M0495L     -20    
        Hot Start Taq DNA Polymerase M0495LVIAL -20 1 x 0.2 ml 5,000 units/ml
        Standard Taq Reaction Buffer Pack B9014SVIAL -20 3 x 1.5 ml 10 X

  • 特性和用法

    单位定义

    One unit is defined as the amount of enzyme that will incorporate 15 nmol of dNTP into acid insoluble material in 30 minutes at 75°C.

    反应条件

    1X Standard Taq Reaction Buffer Pack

    1X Standard Taq Reaction Buffer Pack
    10 mM Tris-HCl
    50 mM KCl
    1.5 mM MgCl2
    (pH 8.3 @ 25°C)

    贮存溶液

    10 mM Tris-HCl
    100 mM KCl
    1 mM DTT
    0.1 mM EDTA
    50% Glycerol
    1X stabilizers
    pH 7.4 @ 25°C

    热失活

    否

    单位活性检测条件

    1X ThermoPol® Reaction Buffer, 200 µM dNTPs including [3H]-dTTP and 15 nM primed M13 DNA.

  • 优势和特性

    应用特性

    • High-specificity PCR
    • Routine PCR
    • Microarray Analysis
    • Colony PCR

  • 相关产品

    相关产品

    • Deoxynucleotide (dNTP) Solution Set
    • Deoxynucleotide (dNTP) Solution Mix
    • Hot Start Taq 2X Master Mix
    • Magnesium Chloride (MgCl2) Solution
    • Standard Taq Reaction Buffer Pack

  • 参考文献
    1. Chien, A., Edgar, D.B. and Trela, J.M. (1976). J. Bact.. 127, 1550-1557.
    2. Kaledin, A.S., Slyusarenko, A.G. and Gorodetskii, S.I. (1980). Biokhimiya. 45, 644-651.
    3. Lawyer, F.C. et al. (1993). PCR Methods and Appl.. 2, 275-287.
    4. Longley, M.J., Bennett, S.E. and Mosbaugh D.W. (1990). Nucleic Acids Res.. 18, 7317-7322.
    5. Lyamichev, V., Brow, M.A. and Dahlberg, J.E. (1993). Science. 260, 778-783.
    6. Sun, Y., Hegamyer, G. and Colburn, N. (1993). Biotechniques. 15, 372-374.
    7. Sarkar, G., Kapelner, S. and Sommer, S.S. (1990). Nucleic Acids Res. 18, 7465.

操作说明、说明书 & 用法

  • 操作说明
    1. PCR Using Hot Start Taq DNA Polymerase (M0495)
    2. A-Tailing with Taq Polymerase

  • 使用指南
    • Activity of Restriction Enzymes in PCR Buffers

工具 & 资源

  • Web 工具
    • Tm Calculator

FAQs & 问题解决指南

  • FAQs
    1. Does Hot Start Taq DNA Polymerase require an extended initial incubation to activate the polymerase?
    2. How should I set up a PCR using Hot Start Taq DNA Polymerase?
    3. Can Hot Start Taq DNA Polymerase be used in other buffers?
    4. What type of DNA ends result from a primer extension reaction or a PCR reaction using Hot Start Taq DNA Polymerase?
    5. When should Hot Start Taq DNA Polymerase be used in a primer extension reaction or for PCR?
    6. Will the 5’→3′ flap endonuclease activity of Hot Start Taq DNA Polymerase degrade primers?
    7. Can Hot Start Taq DNA Polymerase be used for nick translation?
    8. How should I determine an appropriate annealing temperature for my reaction?
    9. What is the longest amplicon that can be obtained with Hot Start Taq DNA Polymerase?
    10. Can Hot Start Taq DNA Polymerase be used with uracil-containing primers or bisulfite-treated DNA?

引用 & 技术文献

  • 引用文献

    产品引用文献查找工具

    Hot Start Taq DNA Polymerase | Powered by Bioz See more details on Bioz

  • 专题文章
    • Using aptamers to control enzyme activities: Hot Start Taq and beyond

质控、安全 & 法规

  • 质控分析
    每一新批次的 NEB 产品都要经过质控，以满足指定的质量标准，对特定产品的质量标准和每一批次的检测数据可以通过产品说明表格、COA、产品信息卡或者产品手册进行查阅或下载。关于 NEB 产品质控的详细信息，可从此处查阅。

  • 产品说明与变更通知

    产品说明与变更通知

    产品说明表包含产品的储存温度、有效期和规格，这些文件的命名规则如下：[货号]_[规格]_[版本]

    • M0495S_L_v1
    • M0495S_L_v2

  • CoA
    CoA 文件包含单个批次产品的储存温度、有效期和质控，这些文件的命名规则如下： [货号]_[规格]_[版本]_[Lot#]

    • M0495S_L_v1_0011509
    • M0495S_L_v1_0011603
    • M0495S_L_v1_0031608
    • M0495S_L_v1_0031703
    • M0495S_L_v1_0031709
    • M0495S_L_v1_0031803
    • M0495S_v1_10011369
    • M0495L_v1_10015893
    • M0495L_v1_10019647
    • M0495S_v1_10015904
    • M0495L_v1_10032782
    • M0495L_v1_10036030
    • M0495L_v1_10040039
    • M0495S_v1_10042556
    • M0495L_v1_10047883
    • M0495S_v1_10047884
    • M0495L_v1_10054111
    • M0495L_v1_10055901
    • M0495L_v1_10058437
    • M0495L_v1_10062497
    • M0495S_v1_10061071
    • M0495L_v2_10108735
    • M0495S_v2_10120886
    • M0495L_v2_10121217
    • M0495S_v2_10141009
    • M0495L_v2_10150849
    • M0495S_v2_10162272

  • SDS
    以下 SDS 文件可以帮助您安全地使用该产品

    • Hot Start Taq DNA Polymerase
    • Standard Taq Reaction Buffer Pack