OneTaq® 2X Master Mix with Standard Buffer |NEB酶试剂 New England Biolabs

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OneTaq® 2X Master Mix with Standard Buffer

OneTaq® DNA Polymerase is an optimized blend of Taq and Deep Vent® DNA polymerases for use with routine and difficult PCR experiments.

  • Obtain high yields across a wide range of AT / GC content
  • 2X higher fidelity than Taq
  • Master mix is a 2X concentrated solution containing everything needed for robust amplification


OneTaq® 2X Master Mix with Standard Buffer |

How to Amplify GC-rich DNA


货号 浓度 规格 目录价 北京 上海 广州 成都 苏州
M0482S 2 X 100 reactions (50 µl) ¥429.00
M0482L 2 X 500 reactions (50 µl) ¥1,889.00
Please enter a quantity for at least one size


OneTaq® 2X Master Mix with Standard Buffer is an optimized blend of Taq and Deep Vent® DNA Polymerases ideally suited to routine PCR applications from a variety of templates including pure DNA solutions, bacterial colonies, and cDNA products. The 3´→5´ exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robust amplification of Taq DNA Polymerase (1). The convenient master mix formulation contains dNTPs, MgCl2, buffer components and stabilizers, requiring only the addition of primers and DNA template for robust amplification.

Comparison of OneTaq Products to Other Commercially Available Polymerases

OneTaq® 2X Master Mix with Standard Buffer | 

Amplification of a selection of high GC human genomic DNA targets demonstrates OneTaq performance. All polymerases were cycled according to manufacturer’s recommendations, including the use of additives to enhance the amplification of targets with high GC content. Yield (dot size) and purity (color) of reaction product were quantified from triplicate reactions on a Perkin Elmer LabChip®. A large, dark green dot represents the highest yield and purity.


OneTaq® 2X Master Mix with Standard Buffer |


An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1 and an E. coli strain that carries the Deep Vent® DNA Polymerase gene from Pyrococcus species GB-D.

OneTaq® DNA Polymerases Products,
Taq DNA Polymerase Products,
Master Mixes Products

Fast PCR,
Multiplex PCR,
Specialty PCR,

Routine PCR,


  • 产品组分信息



    NEB # 名称 组分货号 储存温度 数量 浓度
    • M0482S     -20    
        OneTaq® 2X Master Mix with Standard Buffer M0482SVIAL -20 2 x 1.25 ml 2 X
    • M0482L     -20    
        OneTaq® 2X Master Mix with Standard Buffer M0482SVIAL -20 10 x 1.25 ml 2 X

  • 特性和用法

    1X 预混液组分

    20 mM Tris-HCl
    22 mM KCl
    22 mM NH4Cl
    1.8 mM MgCl2
    5% Glycerol
    0.05% Tween® 20
    0.06% IGEPAL® CA-630
    0.2 mM dNTPs
    25 units/ml OneTaq® DNA Polymerase
    pH 8.9 @ 25°C



    1X ThermoPol® Reaction Buffer, 200 µM dNTPs including [3H]-dTTP and 15 nM primed M13 DNA. 

  • 优势和特性


    • GC-rich PCR 
    • High Sensitivity PCR
    • High Throughput PCR 
    • Colony PCR 
    • Long PCR (up to ~6 kb genomic)
    • AT-rich PCR

  • 相关产品


    • Magnesium Chloride (MgCl2) Solution
    • OneTaq® DNA Polymerase
    • OneTaq® Hot Start DNA Polymerase
    • OneTaq® Quick-Load® 2X Master Mix with Standard Buffer
    • OneTaq® Hot Start 2X Master Mix with Standard Buffer

  • 注意事项
    1. OneTaq 2X Master Mix with Standard Buffer is stable for fifteen freeze-thaw cycles when stored at -20°C.
    2. OneTaq 2X Master Mix with Standard Buffer is also stable for one month at 4°C, so for frequent use, an aliquot may be kept at 4°C.
    3. Product specifications for individual components in the OneTaq 2X Master Mix with Standard Buffer are available separately.

  • 参考文献
    1. Barnes,W.M. (1994). Proc. Natl Acad. Sci. USA. 91, 2216-2220.
    2. Saiki,R.K. et al (1985). Science. 230, 1350-1354.
    3. Powell,L.M. et. al. (1987). Cell. 50, 831-840.
    4. Sun,y., Hegamyer, G. and Colburn,N. (1993). Biotechniques. 15, 372-374.
    5. Sarker, G., Kapelner, S. and Summer, S.S. (1990). Nuclear Acids Res.. 18, 7465.
    6. Magda Matoušková, Pavel Veselý, Petr Daniel, Giada Mattiuzzo, Ralph Hector, Linda Scobie, Yasuhiro Takeuchi and Jiří Hejnar (2013). The Role of DNA Methylation in Expression and Transmission of Procine Endogenous Retrovirus. Journal of Virology. JVI.03262-12,

操作说明、说明书 & 用法

  • 操作说明
    1. Protocol for OneTaq® 2X Master Mix with Standard Buffer (M0482)

  • 使用指南
    • Activity of Restriction Enzymes in PCR Buffers
    • Guidelines for PCR Optimization with OneTaq® and OneTaq® Hot Start DNA Polymerases
    • Guidelines for PCR Optimization with Thermophilic DNA Polymerases

  • 应用实例
    • Robust Colony PCR from Multiple E. coli Strains using OneTaq® Quick-Load® Master Mixes

工具 & 资源

  • 选择指南
    • DNA Polymerase Selection Chart
    • Thermophilic DNA Polymerases

  • Web 工具
    • Tm Calculator

FAQs & 问题解决指南

  • FAQs
    1. How should I set up a PCR using the OneTaq® Master Mixes?
    2. Can I use my regular Taq-based cycling conditions for OneTaq® DNA Polymerase based products?
    3. Can I use OneTaq® DNA Polymerase in “hot start” PCR to help with specificity or primer dimer problems?
    4. What are the stability and storage requirements of the OneTaq® Master Mixes?
    5. Can OneTaq® DNA Polymerase be used in colony PCR?
    6. What type of DNA ends result from a primer extension reaction or a PCR using OneTaq® DNA Polymerase?
    7. How long a product can be made by OneTaq® DNA Polymerase?
    8. Can OneTaq® DNA Polymerase be used with uracil-containing primers or bisulfite-treated DNA?
    9. What is the fidelity of OneTaq® DNA Polymerase?
    10. Where can I find help troubleshooting my PCR?
    11. How should I determine an appropriate annealing temperature for my reaction?
    12. How is OneTaq DNA Polymerase different from LongAmp™ Taq DNA Polymerase?

  • 问题解决指南
    • PCR Troubleshooting Guide

  • 实验技巧
    Use OneTaq 2X Master Mix with Standard Buffer to amplify routine targets with up to 65% GC content.

引用 & 技术文献

  • 引用文献


    OneTaq® 2X Master Mix with Standard Buffer | Powered by Bioz See more details on Bioz


    • Stöhr AC, López-Bueno A, Blahak S, Caeiro MF, Rosa GM, Alves de Matos AP, Martel A, Alejo A, Marschang RE (2015) Phylogeny and differentiation of reptilian and amphibian ranaviruses detected in europe PLoS One; 10(2), e0118633. PubMedID: 25706285, DOI: 10.1371/journal.pone.0118633

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  • 质控分析
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  • 产品说明与变更通知



    • M0482S_L_v1
    • M0482S_L_v2

  • CoA
    CoA 文件包含单个批次产品的储存温度、有效期和质控,这些文件的命名规则如下: [货号]_[规格]_[版本]_[Lot#]

    • M0482S_L_v1_0141612
    • M0482S_L_v1_0151706
    • M0482S_L_v1_0161712
    • M0482L_v1_10008731
    • M0482L_v1_10015061
    • M0482S_v1_10014196
    • M0482L_v1_10019978
    • M0482S_v1_10026511
    • M0482S_v1_10029430
    • M0482L_v1_10026510
    • M0482S_v1_10032470
    • M0482L_v1_10036682
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    • M0482L_v1_10039310
    • M0482S_v1_10036683
    • M0482L_v1_10042494
    • M0482L_v1_10047023
    • M0482S_v1_10047024
    • M0482L_v1_10048754
    • M0482S_v1_10050684
    • M0482S_v1_10051130
    • M0482L_v1_10051129
    • M0482L_v1_10057774
    • M0482S_v1_10057771
    • M0482L_v1_10061437
    • M0482L_v1_10062520
    • M0482L_v2_10068553
    • M0482S_v2_10068784
    • M0482L_v2_10068785
    • M0482L_v2_10071663
    • M0482L_v2_10076981
    • M0482L_v2_10080289
    • M0482S_v2_10082678
    • M0482L_v2_10082885
    • M0482L_v2_10088143
    • M0482S_v2_10088142
    • M0482S_v2_10093280
    • M0482L_v2_10093281
    • M0482S_v2_10100280
    • M0482L_v2_10100279
    • M0482S_v2_10100564
    • M0482L_v2_10104365
    • M0482L_v2_10106904
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    • M0482S_v2_10108349
    • M0482L_v2_10109701
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    • M0482L_v2_10115540
    • M0482L_v2_10121320
    • M0482S_v2_10122328
    • M0482L_v2_10125363
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    • M0482L_v2_10129101
    • M0482L_v2_10130651
    • M0482L_v2_10132264
    • M0482S_v2_10135938
    • M0482L_v2_10137223
    • M0482S_v2_10141227
    • M0482L_v2_10146099
    • M0482S_v2_10146682
    • M0482L_v2_10148706
    • M0482S_v2_10151824
    • M0482L_v2_10151437
    • M0482L_v2_10159689
    • M0482S_v2_10159711
    • M0482S_v2_10163748

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    • OneTaq® 2X Master Mix with Standard Buffer