Q5® Hot Start High-Fidelity DNA Polymerase |NEB酶试剂 New England Biolabs

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Q5® Hot Start High-Fidelity DNA Polymerase

Fidelity at its finest

Q5® High-Fidelity DNA Polymerase sets a new standard for both fidelity and robust performance. With the highest fidelity amplification available (~280 times higher than Taq), Q5 DNA Polymerase results in ultra-low error rates. Q5 DNA Polymerase is composed of a novel polymerase that is fused to the processivity-enhancing Sso7d DNA binding domain, improving speed, fidelity and reliability of performance.

Working with uracil-containing DNA templates or using dUTP? Learn about Q5U Hot Start High-Fidelity DNA Polymerase (NEB #M0515).

  • Highest fidelity amplification (~280X higher than Taq)
  • Ultra-low error rates
  • Superior performance for a broad range of amplicons (from high AT to high GC)
  • Hot start and master mix formats available


Q5® Hot Start High-Fidelity DNA Polymerase |

Important Tips for Q5® Polymerase


货号 浓度 规格 目录价 北京 上海 广州 成都 苏州
M0493S 2,000 units/ml 100 units ¥1,179.00
M0493L 2,000 units/ml 500 units ¥4,659.00
Please enter a quantity for at least one size

Q5® Hot Start High-Fidelity DNA Polymerase | Interested in glycerol-free or custom formulations? – contact our Custom Solutions Group

Bulk packaging may also be available and requested for large recurring orders.
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Q5® Hot Start High-Fidelity DNA Polymerase |


Q5® Hot Start High-Fidelity DNA Polymerase is a high-fidelity, thermostable, hot start DNA polymerase with 3´→ 5´ exonuclease activity, fused to a processivity-enhancing Sso7d domain to support robust DNA amplification. The addition of an aptamer-based inhibitor allows room temperature reaction setup. With an error rate ~280-fold lower than that of Taq DNA Polymerase, Q5 Hot Start High-Fidelity DNA Polymerase is ideal for cloning and can be used for long or difficult amplicons. Q5 Hot Start High-Fidelity DNA Polymerase is supplied with an optimized buffer system that allows robust amplification regardless of GC content. The 5X Q5 Reaction Buffer contains 2 mM Mg++ at final (1X) reaction concentrations and is recommended for most routine applications. For GC-rich targets (≥ 65% GC), amplification can be improved by the addition of the 5X Q5 High GC Enhancer. Q5 Hot Start High-Fidelity DNA Polymerase is unlike typical, lower fidelity PCR enzymes. To determine the optimal annealing temperatures for a given set of primers, use of the NEB Tm Calculator is highly recommended.

Q5® Hot Start High-Fidelity DNA Polymerase |
Amplification of a variety of human genomic amplicons from low to high GC content using Q5 Hot Start High-Fidelity DNA Polymerase. All reactions were set up at room temperature, conducted using 30 cycles of amplification and visualized by microfluidic LabChip® analysis.


Q5® Hot Start High-Fidelity DNA Polymerase |


An E. coli strain that carries the Q5 High-Fidelity DNA Polymerase gene.

Q5® High-Fidelity DNA Polymerases Products

Genome Editing Applications,
Hot Start PCR,
Long Range PCR,

Fast PCR,
High-Fidelity PCR,
Multiplex PCR,
DNA Amplification, PCR & qPCR,
Specialty PCR,
Routine PCR,

Fast Cloning: Accelerate your cloning workflows with reagents from NEB

  • 产品组分信息



    NEB # 名称 组分货号 储存温度 数量 浓度
    • M0493S     -20    
        Q5® Hot Start High-Fidelity DNA Polymerase M0493SVIAL -20 1 x 0.05 ml 2,000 units/ml
        Q5® Reaction Buffer Pack B9027SVIAL -20 1 x 1.5 ml 5 X
        Q5® High GC Enhancer B9028AVIAL -20 1 x 1.5 ml 5 X
    • M0493L     -20    
        Q5® Hot Start High-Fidelity DNA Polymerase M0493LVIAL -20 1 x 0.25 ml 2,000 units/ml
        Q5® Reaction Buffer Pack B9027SVIAL -20 4 x 1.5 ml 5 X
        Q5® High GC Enhancer B9028AVIAL -20 4 x 1.5 ml 5 X

  • 特性和用法


    One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 74°C.



    25 mM TAPS-HCl (pH 9.3 @ 25°C), 50 mM KCl, 2 mM MgCl2, 1 mM β-mercaptoethanol, 200 μM dNTPs including [3H]-dTTP and 15 nM primed M13 DNA.

  • 优势和特性


    • High-specificity PCR 
    • High-fidelity PCR
    • Cloning
    • Long or Difficult Amplification
    • High-throughput PCR

  • 相关产品


    • Deoxynucleotide (dNTP) Solution Mix
    • Deoxynucleotide (dNTP) Solution Set
    • Q5® High-Fidelity 2X Master Mix
    • Q5® High-Fidelity DNA Polymerase
    • Q5® Hot Start High-Fidelity 2X Master Mix
    • Q5® Reaction Buffer Pack

操作说明、说明书 & 用法

  • 操作说明
    1. PCR Using Q5® Hot Start High-Fidelity DNA Polymerase (M0493)

  • 使用指南
    • Activity of Restriction Enzymes in PCR Buffers
    • Guidelines for PCR Optimization with Thermophilic DNA Polymerases

  • 应用实例
    • AppNote_Multiplex_PCR_Using_Q5_HF_DNA_Polymerase

工具 & 资源

  • 选择指南
    • DNA Polymerase Selection Chart

  • Web 工具
    • Tm Calculator

FAQs & 问题解决指南

  • FAQs
    1. What are the advantages to using Q5® Hot Start High-Fidelity DNA Polymerase?
    2. What is the fidelity of Q5® High-Fidelity DNA Polymerase?
    3. How should I determine an appropriate annealing temperature for my reaction?
    4. What should my primer concentration be when using Q5® High-Fidelity DNA Polymerase products?
    5. How should I set up a PCR reaction using Q5® Hot Start High-Fidelity DNA Polymerase?
    6. My template is GC rich or supercoiled. How can I optimize my product yield using Q5® High-Fidelity DNA Polymerase?
    7. Do I need to modify my annealing temperature when using the Q5® High GC Enhancer?
    8. When should I add the High GC Enhancer?
    9. How do I activate Q5® Hot Start High-Fidelity DNA Polymerase?
    10. Are the DNA fragments produced by Q5® High-Fidelity DNA Polymerase blunt-ended or do they have the single-base 3´ overhang that Taq DNA Polymerase yields?
    11. There is a precipitate in the bottom of the buffer tube. Is this normal?
    12. What length of product can be made by Q5® High-Fidelity DNA Polymerase?
    13. I am having trouble amplifying a template that is longer than 5kb. How can I optimize my product yield using Q5® High-Fidelity DNA Polymerase?
    14. Does Q5® High-Fidelity DNA Polymerase exhibit a strand displacement activity?
    15. Where can I find help troubleshooting my PCR?
    16. Will Q5® High-Fidelity DNA Polymerase incorporate dUTPs?
    17. I’d like to clone a fragment amplified with Q5® High-Fidelity DNA Polymerase. Do I have to blunt-end clone?
    18. Do other polymerases work in Q5® Reaction Buffer?
    19. What is the difference between Q5 and Q5U Hot Start High-Fidelity DNA Polymerase?

  • 问题解决指南
    • PCR Troubleshooting Guide

引用 & 技术文献

  • 引用文献


    Q5® Hot Start High-Fidelity DNA Polymerase | Powered by Bioz See more details on Bioz


    • Stoczynska-Fidelus E, Och W, Rieske P, Bienkowski M, Banaszczyk M, Winiecka-Klimek M, Zieba J, Janik K, Rosiak K, Treda C, Stawski R, Radomiak-Zaluska A, Piaskowski S (2014) Spontaneous in vitro senescence of glioma cells confirmed by an antibody against IDH1R132H Anticancer Res; 34(6), 2859-67. PubMedID: 24922649
    • Currin A, Swainston N, Day PJ, Kell DB (2014) SpeedyGenes: an improved gene synthesis method for the efficient production of error-corrected, synthetic protein libraries for directed evolution Protein Eng Des Sel; 27(9), 273-80. PubMedID: 25108914, DOI: 10.1093/protein/gzu029
    • Toru Takahashi, Ken Maeda, Tadaki Suzuki, Aki Ishido, Toru Shigeoka, Takayuki Tominaga, Toshiaki Kamei, Masahiro Honda, Daisuke Ninomiya, Takenori Sakai, Takanori Senba, Shozo Kaneyuki, Shota Sakaguchi, Akira Satoh, Takanori Hosokawa, Yojiro Kawabe, Shintaro Kurihara, Koichi Izumikawa, Shigeru Kohno, Taichi Azuma, Koichiro Suemori, Masaki Yasukawa, Tetsuya Mizutani, Tsutomu Omatsu, Yukie Katayama, Masaharu Miyahara, Masahito Ijuin, Kazuko Doi, Masaru Okuda, Kazunori Umeki, Tomoya Saito, Kazuko Fukushima, Kensuke Nakajima, Tomoki Yoshikawa, Hideki Tani, Shuetsu Fukushi, Aiko Fukuma, Momoko Ogata, Masayuki Shimojima, Noriko Nakajima, Noriyo Nagata, Harutaka Katano, Hitomi Fukumoto, Yuko Sato, Hideki Hasegawa, Takuya Yamagishi, Kazunori Oishi, Ichiro Kurane, Shigeru Morikawa, Masayuki Saijo (2013) The First Identification and Retrospective Study of Severe Fever With Thrombocytopenia Syndrome in Japan J Inf Dis; 209(6), 816-827. PubMedID: 24231186

  • 出版物
    • Juliane Krebes, Richard D. Morgan, Boyke Bunk, Cathrin Spröer, Khai Luong, Raphael Parusel, Brian P. Anton, Christoph König, Christine Josenhans, Jörg Overmann, Richard J. Roberts, Jonas Korlach, Sebastian Suerbaum (2013) The complex methylome of the human gastric pathogen Helicobacter pylori Nucleic Acids Res; 42(4), 2415-2432. PubMedID: 24302578, DOI: 10.1093/nar/gkt1201

  • 专题文章
    • Polymerase Fidelity: What is it, and what does it mean for your PCR?
    • Using aptamers to control enzyme activities: Hot Start Taq and beyond

质控、安全 & 法规

  • 质控分析
    每一新批次的 NEB 产品都要经过质控,以满足指定的质量标准,对特定产品的质量标准和每一批次的检测数据可以通过产品说明表格、COA、产品信息卡或者产品手册进行查阅或下载。关于 NEB 产品质控的详细信息,可从此处查阅。

  • 产品说明与变更通知



    • M0493S_L_v1

  • CoA
    CoA 文件包含单个批次产品的储存温度、有效期和质控,这些文件的命名规则如下: [货号]_[规格]_[版本]_[Lot#]

    • M0493S_L_v1_0121606
    • M0493S_L_v1_0071505
    • M0493S_L_v1_0111512
    • M0493S_L_v1_0151612
    • M0493S_L_v1_0151706
    • M0493S_L_v1_0191712
    • M0493L_v1_10007543
    • M0493L_v1_10014733
    • M0493S_v1_10017673
    • M0493L_v1_10021411
    • M0493S_v1_10022245
    • M0493L_v1_10025839
    • M0493S_v1_10026171
    • M0493L_v1_10032192
    • M0493S_v1_10029216
    • M0493L_v1_10034496
    • M0493S_v1_10036747
    • M0493L_v1_10036748
    • M0493L_v1_10042498
    • M0493S_v1_10040297
    • M0493L_v1_10045790
    • M0493L_v1_10047950
    • M0493S_v1_10047831
    • M0493L_v1_10049885
    • M0493L_v1_10051820
    • M0493S_v1_10051814
    • M0493L_v1_10055338
    • M0493S_v1_10057910
    • M0493L_v1_10057912
    • M0493L_v1_10060871
    • M0493S_v1_10060286
    • M0493L_v1_10063522
    • M0493L_v1_10065197
    • M0493S_v1_10067254
    • M0493L_v1_10071140
    • M0493L_v1_10075123
    • M0493L_v1_10077342
    • M0493S_v1_10077190
    • M0493S_v1_10078997
    • M0493L_v1_10079277
    • M0493L_v1_10082201
    • M0493L_v1_10085116
    • M0493S_v1_10083166
    • M0493L_v1_10088131
    • M0493L_v1_10089130
    • M0493S_v1_10088152
    • M0493S_v1_10093297
    • M0493L_v1_10093298
    • M0493L_v1_10098032
    • M0493S_v1_10098036
    • M0493L_v1_10100657
    • M0493S_v1_10100656
    • M0493L_v1_10102587
    • M0493L_v1_10106979
    • M0493S_v1_10103356
    • M0493L_v1_10109421
    • M0493L_v1_10111051
    • M0493L_v1_10112649
    • M0493S_v1_10110303
    • M0493S_v1_10114447
    • M0493L_v1_10114919
    • M0493S_v1_10119830
    • M0493L_v1_10118912
    • M0493L_v1_10122244
    • M0493L_v1_10127960
    • M0493S_v1_10127959
    • M0493L_v1_10131148
    • M0493L_v1_10134956
    • M0493L_v1_10138439
    • M0493S_v1_10140760
    • M0493S_v1_10145147
    • M0493L_v1_10144277
    • M0493L_v1_10148895
    • M0493L_v1_10151327
    • M0493S_v1_10153091
    • M0493L_v1_10155132
    • M0493S_v1_10157140

  • SDS
    以下 SDS 文件可以帮助您安全地使用该产品

    • Q5® Hot Start High-Fidelity DNA Polymerase
    • Q5® Reaction Buffer Pack
    • Q5® High GC Enhancer