Q5® High-Fidelity PCR Kit |NEB酶试剂 New England Biolabs

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Q5® High-Fidelity PCR Kit

Fidelity at its finest

Q5® High-Fidelity DNA Polymerase sets a new standard for both fidelity and robust performance. With the highest fidelity amplification available (~280 times higher than Taq), Q5 DNA Polymerase results in ultra-low error rates. Q5 DNA Polymerase is composed of a novel polymerase that is fused to the processivity-enhancing Sso7d DNA binding domain, improving speed, fidelity and reliability of performance.

  • Highest fidelity amplification (~280X higher than Taq)
  • Ultra-low error rates
  • Superior performance for a broad range of amplicons (from high AT to high GC)
  • Hot Start and Master Mix formats available

Q5® High-Fidelity PCR Kit |

库存
货号 浓度 规格 目录价 北京 上海 广州 成都 苏州
E0555S 不适用 50 reactions ¥919.00 有 有 有 有 有
E0555L 不适用 200 reactions ¥2,799.00 有 有 无 无 无
如何订购
Please enter a quantity for at least one size

Q5® High-Fidelity PCR Kit | Need a custom/large volume order? Contact Us

Bulk packaging may also be available and requested for large recurring orders.
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产品信息

Q5® High-Fidelity PCR Kit |  

Q5® High-Fidelity DNA Polymerase is an ultra high-fidelity, thermostable DNA polymerase with 3´→ 5´ exonuclease activity, fused to a processivity-enhancing Sso7d domain to support robust DNA amplification. With an error rate ~280-fold lower than that of Taq DNA Polymerase, Q5 High-Fidelity DNA Polymerase is ideal for cloning and can be used for long or difficult amplicons. The Q5 High-Fidelity 2X Master Mix offers robust, high-fidelity performance in a convenient master mix format. The mix contains dNTPs, Mg++ and a proprietary broad-use buffer requiring only the addition of primers and DNA template for robust amplification, regardless of GC content. When used at the recommended 1X final concentration, the Q5 High-Fidelity Master Mix contains 2 mM MgCl2.

Q5 High-Fidelity DNA Polymerase is unlike typical, lower fidelity PCR enzymes. To determine the optimal annealing temperatures for a given set of primers, use of the NEB Tm Calculator is highly recommended.

This kit contains a sufficient supply of master mix and nuclease-free water to perform 50 polymerase chain reactions (NEB #E0555S) or 200 reactions (NEB #E0555L). For added convenience, gel loading dye, and a Quick-Load DNA LAdder are also included in this kit.

Q5® High-Fidelity PCR Kit |

Q5® High-Fidelity PCR Kit |

产品类别:
Q5® High-Fidelity DNA Polymerases Products

应用:
Long Range PCR,
Fast PCR,
High-Fidelity PCR,

Multiplex PCR,
DNA Amplification, PCR & qPCR,
Specialty PCR,
Routine PCR,
PCR,

Fast Cloning: Accelerate your cloning workflows with reagents from NEB

  • 试剂盒组成

    试剂盒组成

    本产品提供以下试剂或组分：

    NEB # 名称 组分货号 储存温度 数量 浓度
    • E0555S     -20    
        Q5® High-Fidelity 2X Master Mix M0492SVIAL -20 1 x 1.25 ml 2 X
        Quick-Load® Purple 1 kb Plus DNA Ladder N0550AVIAL 4 1 x 0.2 ml 100 µg/ml
        Gel Loading Dye, Purple (6X) B7024SVIAL 25 1 x 1 ml 6 X
        Nuclease-free Water B1502AVIAL -20 1 x 1.5 ml 不适用
    • E0555L     -20    
        Q5® High-Fidelity 2X Master Mix M0492SVIAL -20 4 x 1.25 ml 2 X
        Quick-Load® Purple 1 kb Plus DNA Ladder N0550AAVIAL -20 1 x 0.4 ml 100 µg/ml
        Gel Loading Dye, Purple (6X) B7024SVIAL 25 1 x 1 ml 6 X
        Nuclease-free Water B1502AVIAL -20 4 x 1.5 ml 不适用

  • 特性和用法

  • 相关产品

    相关产品

    • Deoxynucleotide (dNTP) Solution Mix
    • Deoxynucleotide (dNTP) Solution Set
    • Q5® High-Fidelity DNA Polymerase
    • Q5® Hot Start High-Fidelity 2X Master Mix
    • Q5® Hot Start High-Fidelity DNA Polymerase
    • Q5® Reaction Buffer Pack

  • 注意事项
    1. Product specifications for individual components in the Q5 High-Fidelity PCR Kit are available separately.

  • 参考文献
    1. Anastassia Voronova Erin Coyne, Ashraf Al Madhoun, Joel V. Fair, Neven Bosiljcic, Catherine St-Louis, Grace Li, Sherry Thurig, Valerie A. Wallace, Nadine Wiper-Bergeron, and Ilona S. Skerjanc. (2013). Hedgehog Signaling Regulates MyoD Expression and Activity. J Biol Chem. 288(6), 4389–4404. PubMedID: PMC3567689
    2. Lieve Naesens, Luke Guddat, Dianne Keough, André B.P. van Kuilenburg, Judith Meijer, Johan Vande Voorde and Jan Balzarini (2013). ROLE OF HUMAN HYPOXANTHINE GUANINE PHOSPHORIBOSYLTRANSFERASE IN ACTIVATION OF THE ANTIVIRAL AGENT T-705 (FAVIPIRAVIR). Molecular Pharmacology Fast Forward. 87247,
    3. Hicham Bouabe, Klaus Okkenhaug. (2013). A Protocol for Construction of Gene Targeting Vectors and Generation of Homologous Recombinant Embryonic Stem Cells. Methods in Molecular Biology . 1064, 337-354.

操作说明、说明书 & 用法

  • 操作说明
    1. Protocol for a Routine PCR (E0555)
    2. PCR Optimization (E0555)

  • 说明书
    产品说明书包含产品使用的详细信息、产品配方和质控分析。

    • manualE0555

  • 使用指南
    • Activity of Restriction Enzymes in PCR Buffers

工具 & 资源

  • Web 工具
    • Tm Calculator

FAQs & 问题解决指南

  • FAQs
    1. What are the advantages to using Q5® High-Fidelity 2X Master Mix?
    2. What is the fidelity of Q5® High-Fidelity DNA Polymerase?
    3. How should I determine an appropriate annealing temperature for my reaction?
    4. What should my primer concentration be when using Q5® High-Fidelity DNA Polymerase products?
    5. How should I set up a PCR experiment using Q5® High-Fidelity 2X Master Mix?
    6. My template is GC rich or supercoiled. How can I optimize my product yield using Q5® High-Fidelity Master Mixes?
    7. I have a tube of the Q5® High GC Enhancer from another product formulation – can I add it to the Q5 Master Mix?
    8. There is a precipitate in the bottom of the Master Mix tube? Is this normal?
    9. What are the stability and storage requirements of the Q5® PCR Kit?
    10. Are the DNA fragments produced by Q5 High-Fidelity DNA Polymerase blunt-ended or do they have the single-base 3´ overhang that Taq DNA Polymerase yields?
    11. What length of product can be made by Q5® High-Fidelity DNA Polymerase?
    12. Does Q5® High-Fidelity DNA Polymerase exhibit a strand displacement activity?
    13. Where can I find additional help troubleshooting my PCR?
    14. Will Q5® High-Fidelity DNA Polymerase incorporate dUTPs?

引用 & 技术文献

  • 引用文献

    产品引用文献查找工具

    Q5® High-Fidelity PCR Kit | Powered by Bioz See more details on Bioz

  • 专题文章
    • Polymerase Fidelity: What is it, and what does it mean for your PCR?

质控、安全 & 法规

  • 质控分析
    每一新批次的 NEB 产品都要经过质控，以满足指定的质量标准，对特定产品的质量标准和每一批次的检测数据可以通过产品说明表格、COA、产品信息卡或者产品手册进行查阅或下载。关于 NEB 产品质控的详细信息，可从此处查阅。

  • 产品说明与变更通知

    产品说明与变更通知

    产品说明表包含产品的储存温度、有效期和规格，这些文件的命名规则如下：[货号]_[规格]_[版本]

    • E0555S_L_v3

  • CoA
    CoA 文件包含单个批次产品的储存温度、有效期和质控，这些文件的命名规则如下： [货号]_[规格]_[版本]_[Lot#]

    • E0555S_L_v1_0131612
    • E0555S_L_v2_0141705
    • E0555S_L_v2_0151712
    • E0555L_v3_10010604
    • E0555S_v3_10010847
    • E0555L_v3_10015261
    • E0555L_v3_10026172
    • E0555S_v3_10027135
    • E0555L_v3_10032078
    • E0555S_v3_10035385
    • E0555S_v3_10042166
    • E0555L_v3_10037380
    • E0555L_v3_10045929
    • E0555S_v3_10045779
    • E0555L_v3_10048550
    • E0555L_v3_10052315
    • E0555S_v3_10055457
    • E0555L_v3_10057919
    • E0555S_v3_10057921
    • E0555S_v3_10063555
    • E0555L_v3_10063899
    • E0555S_v3_10068690
    • E0555L_v3_10068693
    • E0555L_v3_10072265
    • E0555S_v3_10070093
    • E0555L_v3_10076847
    • E0555S_v3_10077367
    • E0555S_v3_10079941
    • E0555L_v3_10079655
    • E0555S_v3_10082196
    • E0555L_v3_10084997
    • E0555S_v3_10084998
    • E0555L_v3_10089154
    • E0555L_v3_10093306
    • E0555S_v3_10090856
    • E0555L_v3_10099477
    • E0555S_v3_10100684
    • E0555L_v3_10104086
    • E0555L_v3_10106233
    • E0555S_v3_10105288
    • E0555L_v3_10109273
    • E0555L_v3_10112842
    • E0555S_v3_10117577
    • E0555L_v3_10118460
    • E0555L_v3_10123535
    • E0555S_v3_10122768
    • E0555L_v3_10127746
    • E0555S_v3_10130706
    • E0555L_v3_10130707
    • E0555S_v3_10137971
    • E0555L_v3_10137972
    • E0555L_v3_10145377
    • E0555S_v3_10148908
    • E0555L_v3_10150661
    • E0555L_v3_10157371
    • E0555S_v3_10157368

  • SDS
    以下 SDS 文件可以帮助您安全地使用该产品

    • Q5® High-Fidelity 2X Master Mix
    • Quick-Load® Purple 1 kb Plus DNA Ladder
    • Gel Loading Dye, Purple (6X)
    • Nuclease-free Water