Tte UvrD Helicase |NEB酶试剂 New England Biolabs

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Tte UvrD Helicase

  • Unwinds double-stranded DNA
  • Thermostable to 65°C
  • Reduces non-specific product formation in isothermal amplification (e.g. LAMP)

This is an Enzyme for Innovation (EFI). EFI is a project initiated by NEB to provide unique enzymes to the scientific community in the hopes of enabling the discovery of new and innovative applications. These enzymes have interesting properties and unique specificities.

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Tte UvrD Helicase |

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库存
货号 浓度 规格 目录价 北京 上海 广州 成都 苏州
M1202S 20 µg/ml 0.5 µg ¥899.00 无 无 有 无 有
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产品信息

Tte UvrD Helicase is a repair helicase capable of unwinding double-stranded DNA, without a requirement for a specific flap or overhang structure, from the thermophilic organism Thermoanaerobacter tengcongensis. It is active on a wide range of DNA substrates and, along with its thermostability (active to 70°C), Tte UvrD Helicase has been demonstrated to be a useful additive for improving specificity of isothermal amplification reactions, particularly in conjunction with the WarmStart® LAMP Kit (DNA & RNA).

Workflow

Tte UvrD Helicase |

Tte UvrD Helicase Eliminates Non-Template Amplification in LAMP Reactions

Tte UvrD Helicase |

LAMP reactions were performed using the WarmStart® LAMP Kit (DNA & RNA) (NEB #E1700) and a primer set prone to high levels of non-template amplification. Without Tte UvrD Helicase (left), the positive reaction (+DNA) amplifies in ~8 minutes while the no-template control (NTC) amplifies in ~20 minutes. In the presence of 10 ng Tte UvrD Helicase (right), the positive reaction maintains its rapid amplification time with a slight reduction in total RFU, while the NTC reaction is completely suppressed.

产品来源

Tte UvrD Helicase is isolated from a strain of E. coli which carries the cloned UvrD gene from Thermoanaerobacter tengcongensis.

产品类别:
PCR, qPCR & Amplification Technologies Products

应用:
Helicase-dependent Amplification,
Loop-Mediated Isothermal Amplification,
DNA Amplification, PCR & qPCR

  • 产品组分信息

    产品组分信息

    本产品提供以下试剂或组分：

    NEB # 名称 组分货号 储存温度 数量 浓度
    • M1202S     -20    
        Tte UvrD Helicase M1202SVIAL -20 1 x 0.025 ml 不适用
        Isothermal Amplification Buffer Pack B0537SVIAL -20 1 x 1.5 ml 10 X
        Adenosine 5′-Triphosphate (ATP) P0756SVIAL -20 1 x 1 ml 10 mM

  • 特性和用法

    反应条件

    1X Isothermal Amplification Buffer Pack
    Supplement with 1 mM ATP
    Incubate at 65°C

    1X Isothermal Amplification Buffer Pack
    20 mM Tris-HCl
    10 mM (NH4)2SO4
    50 mM KCl
    2 mM MgSO4
    0.1% Tween® 20
    (pH 8.8 @ 25°C)

    使用浓度

    0.2 – 0.4 ng/μl

    贮存溶液

    10 mM Tris-HCl
    100 mM KCl
    1 mM DTT
    0.1 mM EDTA
    0.1% Triton® X-100
    50% Glycerol
    pH 7.4 @ 25°C

    热失活

    80°C for 5 min

  • 参考文献
    1. An, L., Tang, W., Ranalli, T.A., Kim, H.J., Wytiaz, J., and Kong, H. (2005). Characterization of a thermostable UvrD helicase and its participation in helicase-dependent amplification. J Biol Chem. 280 (32), 28952-8. PubMedID: 15955821
    2. Mauris, J., and Evans, T.C. Jr. (2010). A human PMS2 homologue from Aquifex aeolicus stimulates an ATP-dependent DNA helicase. J Biol Chem. 285 (15), 11087-92. PubMedID: 20129926
    3. Vincent, M., Xu, Y., and Kong, H. (2004). Helicase-dependent isothermal DNA amplification. EMBO Rep. 5 (8), 795-800. PubMedID: 15247927

操作说明、说明书 & 用法

  • 操作说明
    1. Protocol for LAMP Reactions with Tte UvrD Helicase (#M1202)
    2. Protocol for unwinding double stranded DNA with Tte UvrD Helicase (#M1202)

FAQs & 问题解决指南

  • FAQs
    1. Does Tte UvrD Helicase (NEB #M1202) require any specific DNA structure for unwinding?
    2. How do I use Tte UvrD Helicase for reducing non-template amplification in LAMP reactions?
    3. Is Tte UvrD Helicase compatible with the WarmStart Colorimetric LAMP 2X Master Mix (NEB #M1800)?
    4. Do I need to add ATP for Tte UvrD Helicase activity?
    5. Is Tte UvrD Helicase active in other buffers?
    6. Can I purchase large amounts of an existing Enzyme for Innovation?
    7. What are Enzymes for Innovation?

引用 & 技术文献

  • 引用文献

    产品引用文献查找工具

    Tte UvrD Helicase | Powered by Bioz See more details on Bioz

    更多引用文献

    • Zaczek-Moczydłowska, M.A.; Mohamed-Smith, L.; Toldrà, A.; Hooper, C.; Campàs, M.; Furones, M.D.; Bean, T.P.; Campbell, K. (2020) A Single-Tube HNB-Based Loop-Mediated Isothermal Amplification for the Robust Detection of the Ostreid herpesvirus 1. Int J Mol Sci; 21, 6605,

质控、安全 & 法规

  • 质控分析
    每一新批次的 NEB 产品都要经过质控，以满足指定的质量标准，对特定产品的质量标准和每一批次的检测数据可以通过产品说明表格、COA、产品信息卡或者产品手册进行查阅或下载。关于 NEB 产品质控的详细信息，可从此处查阅。

  • 产品说明与变更通知

    产品说明与变更通知

    产品说明表包含产品的储存温度、有效期和规格，这些文件的命名规则如下：[货号]_[规格]_[版本]

    • M1202S_v1

  • CoA
    CoA 文件包含单个批次产品的储存温度、有效期和质控，这些文件的命名规则如下： [货号]_[规格]_[版本]_[Lot#]

    • M1202_v0_10030797
    • M1202S_v1_10035793
    • M1202S_v1_10041635
    • M1202S_v1_10043679
    • M1202S_v1_10048358
    • M1202S_v1_10062300
    • M1202S_v1_10079151
    • M1202S_v1_10088264
    • M1202S_v1_10091302
    • M1202S_v1_10097606
    • M1202S_v1_10115887
    • M1202S_v1_10157341

  • SDS
    以下 SDS 文件可以帮助您安全地使用该产品

    • Tte UvrD Helicase
    • Isothermal Amplification Buffer Pack
    • Adenosine 5′-Triphosphate (ATP)