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Q5U® Hot Start High-Fidelity DNA Polymerase
Q5U Hot Start High-Fidelity DNA Polymerase is a modified version of Q5® High-Fidelity DNA Polymerase, a novel thermostable DNA polymerase that possesses 3′ to 5′ exonuclease activity, and is fused to a processivity-enhancing Sso7d domain. Q5U contains a mutation in the uracil-binding pocket that enables the ability to read and amplify templates containing uracil and inosine bases.
- Achieve superior amplification of bisulfite-converted, deaminated, or damaged DNA (e.g., FFPE)
- Prevent carryover contamination when combined with dUTP (NEB #N0459) and Uracil DNA Glycosylase (UDG) (NEB #M0372) treatment
- Generate higher assembly efficiency and improve accuracy in USER® cloning
- Enable room temperature setup with aptamer-based hot start formulation
- Utilize optimized protocols for recommended applications
