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Exonuclease V (RecBCD)
- DNA specific exonuclease that also acts as an endonuclease on single-strand DNA
- Initiates at both 5′ and 3′ termini of linear double-stranded DNA
- Cleaves linear double-stranded DNA in both the 3′ to 5′ and 5′ to 3′ directions
- Requires ATP in the reaction
Exonuclease V is ideal for:
- Degradation of contaminating linear DNA in plasmid samples
- Removal of residual gDNA after purification of low copy plasmid
