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Tth Endonuclease IV
- Thermostable DNA AP endonuclease
- Catalyzes the cleavage of DNA phosphodiester backbone at AP sites via hydrolysis, leaving a 1 nucleotide gap with 3′-hydroxyl and 5′ deoxyribose phosphate (dRP) termini
- Also has 3′-diesterase activity which can remove 3′ phosphate, 3′-α, β-unsaturated aldehyde, phosphoglycoaldehyde, and other 3′ blocking groups
- Heat inactivation not possible
