NEBNext^® Ultra™ II Directional RNA Second Strand Synthesis Module

This module is part of the Ultra™ II Directional RNA workflow, for Illumina^®– compatible strand-specific RNA library construction. This workflow is compatible with poly(A) mRNA isolation or ribosomal RNA depletion, and enables high yield preparation of high quality libraries from 5 ng – 1 µg total RNA.

Kits are also available that include reagents for every step in the Ultra II Directional RNA library construction workflow (NEBNext Ultra II Directional RNA Library Prep Kit for Illumina, NEB #E7760 and NEBNext Ultra II Directional RNA Library Prep with Sample Purification Beads, NEB #E7765).