NEBNext^® rRNA Depletion Kit (Human/Mouse/Rat)

Ribosomal RNAs (rRNAs) are extremely abundant, constituting 80–90% of total RNA. Efficient removal of rRNA is critical to enable cost-effective sequencing of RNA samples, but this can be especially challenging with low quality RNA (e.g. FFPE RNA) and with low input amounts. The NEBNext rRNA Depletion kit employs the efficient RNase H method, as well as complete probe tiling of rRNA, thereby ensuring that even degraded rRNA is hybridized and subsequently removed.

• Suitable for low-quality (e.g. FFPE) or high-quality RNA
• Compatible with a broad range of input amounts: 10 ng – 1 μg
• Fast workflow: 2 hours, with less than 10 minutes hands-on time