NEBNext^® RNA First Strand Synthesis Module

This module is optimized for use in the original Ultra™ workflow, for Illumina^®-compatible RNA library construction. The original Ultra workflow enables library preparation from 5 ng – 1 µg of input DNA in a streamlined protocol.

However, we now recommend use of the more recent NEBNext Ultra II Directional RNA and RNA (non-directional) workflows, which have been further optimized to provide increased yields of high quality libraries from a broader range of input amounts, and a NEBNext Ultra II RNA First Strand Synthesis Module (NEB #E7771) is available.