NEBNext^® Multiplex Oligos for Illumina^® (96 Unique Dual Index Primer Pairs)

NEBNext^® Multiplex Oligos provide adaptors and primers to enable high yield multiplex Illumina® library production. The unique hairpin loop structure of the NEBNext Adaptor minimizes adaptor-dimer formation, and NEBNext index PCR primers enable index incorporation during library amplification. This first set of UDI primer pairs (Set 1 of 5) includes 96 pre-mixed unique pairs of i5 and i7 index primers, packaged in a single-use 96-well plate with a pierceable foil seal.

For additional information on available indices and formats for multiplexing, please refer to the NEBNext Multiplex Oligos Selection Chart.

• Allows complete filtering of index-hopped reads
• High efficiency adaptor ligation
• Minimized adaptor-dimer formation
• Includes index primers for library multiplexing
• Increased sample identification specificity
• This set (Set 1) may be combined with sets 2, 3, and 4 for 384 indices