NEBNext^® Ultra^™ II Directional RNA Library Prep Kit for Illumina^®

Even more from less, for RNA

Do you need increased sensitivity and specificity from your RNA-seq experiments? Do you have ever-decreasing amounts of input RNA? To address these challenges, our next generation of strand-specific RNA library prep kits have been reformulated at each step, resulting in several fold higher yields of high quality libraries and enabling use of lower input amounts and fewer PCR cycles. The Ultra II Directional RNA kit uses the “dUTP method” (1, 2) for strand-specificity, and has a streamlined, automatable workflow.

• Generate the highest yields of high quality libraries, with a broad range of input amounts:
  • 10 ng – 1 µg Total RNA (polyA mRNA workflow)
  • 5 ng – 1 µg Total RNA (rRNA depletion workflow)
• Increase the complexity and transcript coverage of your libraries
• Optimize your time with streamlined workflows, reduced hands-on time, and automation compatibility
• Rely on robust performance, even with low quality RNA, including FFPE