NEBNext^® Immune Sequencing Kit (Mouse)

With the NEBNext^® Immune Sequencing Kit (Mouse), sequence the full-length immune gene repertoires of B cells and T cells. Profile somatic mutations across all relevant contexts (e.g., V, D, and J segments and isotypes IgM, IgD, IgG, IgA, and IgE) with improved sequence accuracy. Characterize BCR light, BCR heavy, TCRα, TCRβ, TCRγ and TCRδ chains. This kit includes UMIs for source-molecule identification.

• Unlock the immune system’s complexity with a deeper analysis of receptor sequences
• Enrich for and sequence both B cell receptors (BCR) and T cell receptors (TCR)
• Generate full-length immune gene repertoires of B and T cells
• Accurately quantify transcripts with unique molecular identifiers (UMIs)
• Analyze data using a bioinformatic workflow based on the open-source pRESTO toolkit (tutorial)

Note: We have a separate kit available for immune repertoire sequencing of Human samples.