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Hi-T7 RNA Polymerase (High Concentration)
Hi-T7 RNA Polymerase is designed for in vitro transcription of RNA at higher temperatures.
- Active from 37-56°C, optimal incubation temperature is 50-52°C.
- In vitro transcription using T7 phage promoter
- High concentration version of Hi-T7 RNA Polymerase (NEB #M0658)
- Increased co-transcriptional capping efficiency with cap analogs
- Decreased immunogenicity from RNA synthesized at higher temperature due to reduced dsRNA by-product formation
- Recommended for experienced users with knowledge of transcription optimization
- Hi-T7 RNA Polymerase Reaction buffer and magnesium chloride included

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