EnGen^® Sau Cas9

EnGen^® Sau Cas9 is a programmable DNA endonuclease guided by a single guide RNA (gRNA).  Targeting requires gRNA complementarity to the target site as well as a 5′NNGRRT protospacer adjacent motif (PAM) on the DNA strand opposite the target sequence.  Cleavage by EnGen^® Sau Cas9 occurs ~3 bases upstream of the PAM and leaves blunt ends.

• Longer 5′ NNGRRT 3′ PAM sequence allows for higher specificity
• Two nuclear localization signals for improved transport into the nucleus
• High concentration liquid format option can be used for microinjection, electroporation and lipofection
• Compatible with the EnGen^® Mutation Detection Kit
• Need guide RNA? NEB recommends the Horizon™ CRISPR Design Tool for designing and ordering synthetic guide RNA for EnGen Sau Cas9