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PURExpress® In Vitro Protein Synthesis Kit
PURExpress® is a reconstituted protein synthesis system based on the PUREsystem™ (Shimizu et al., 2001) where all necessary components needed for in vitro transcription and translation are purified from E. coli.
• Defined system with all his-tagged proteins for coupled transcription/translation; Ribosome is not his-tagged
• T7 RNA Polymerase drives in vitro transcription
• Minimal nuclease and protease activity for stability of synthesized protein and encoding target
• Templates can be either plasmid DNA, linear DNA or mRNA
• Protein of interest can be synthesized and visualized in a few hours
• Synthesizes various target peptides and proteins
• Synthesized protein can be co-translationally radiolabeled or fluorescently labeled
• Protein can be reverse-purified or subject to direct functional analysis
• Applications include high throughput screening/directed evolution, synthetic biology, toxic or difficult to express protein synthesis, studies on protein folding, activity and protein-protein interactions
• Due to reconstituted nature, several kits are offered where translation factors or macromolecules have been omitted to facilitate specific studies (see companion products)
• Compatible with the PURExpress Disulfide Bond Enhancer (NEB #E6820)
