NEBExpress^® Cell-free E. coli Protein Synthesis System

The NEBExpress® Cell-free E. coli Protein Synthesis System is an extract-based transcription/translation system derived from E. coli cells that have been engineered for high in vitro synthesis performance. It is designed to synthesize proteins ranging in size from 17-230 kDa in just 2-4 hours under the control of T7 RNA Polymerase.

• Synthesize high yields of protein (typically 0.5 mg/ml) 
• Protein can be synthesized and visualized in approximately 2 – 4 hours
• Synthesize various target proteins ranging from 17 to 230 kDa
• Templates can be plasmid DNA, linear DNA or mRNA.
• Minimal protease activity ensures stability of desired target protein
• RNase contamination can be inhibited by the supplied RNase inhibitor, eliminating clean-up steps
• Protein can be isolated with affinity purification or subject to direct functional analysis
• Reactions can be directly loaded onto an SDS-PAGE gel without the need for acetone or TCA precipitation
• Flexible reaction conditions achieve maximum yield; protein synthesis can be sustained for 10 hours at 37°C or up to 24 hours at lower temperatures
• Reactions can be miniaturized or scaled up
• Compatible with the PURExpress Disulfide Bond Enhancer (NEB #E6820) and NEBExpress GamS Nuclease Inhibitor (NEB #P0774)

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