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pTXB1 is an E. coli expression vector designed for the in-frame insertion of a target gene into the polylinker upstream of the Mxe intein/chitin binding domain. The fusion protein is bound to chitin beads and the thiol-induced cleavage activity of the intein releases the target protein.
- View sequence details
- Used in the IMPACT™ Protein Purification System
- Recommended for use in intein-mediated protein ligation and C-terminal labeling
- Double-stranded vector; 6,706 base pairs in length
- Ampicillin resistance