An affinity matrix for the small-scale isolation of target proteins fused to a chitin binding domain (CBD).

• Chitin beads have been prepared having a magnetite core, thereby permitting the magnetic isolation of CBD-fusion proteins from cell culture supernatants
• The matrix can be regenerated without loss of binding capacity
• Immobilized fusion proteins can be used in subsequent experiments to capture (pull down) target proteins from crude cell lysates that interact with the immobilized fusion protein
• Binding capacity of 2 mg chitin binding domain protein/ml bed volume
• Quick, small-scale purification of CBD-fusion proteins enables higher throughput and efficiency
• Removal of CBD-tag during elution typically yields highly pure, native protein