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Chitin Magnetic Beads
An affinity matrix for the small-scale isolation of target proteins fused to a chitin binding domain (CBD).
- Chitin beads have been prepared having a magnetite core, thereby permitting the magnetic isolation of CBD-fusion proteins from cell culture supernatants
- The matrix can be regenerated without loss of binding capacity
- Immobilized fusion proteins can be used in subsequent experiments to capture (pull down) target proteins from crude cell lysates that interact with the immobilized fusion protein
- Binding capacity of 2 mg chitin binding domain protein/ml bed volume
- Quick, small-scale purification of CBD-fusion proteins enables higher throughput and efficiency
- Removal of CBD-tag during elution typically yields highly pure, native protein
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