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p-Nitrophenyl Phosphate (PNPP)
p-Nitrophenyl Phosphate (PNPP) is a non-proteinaceous, non-specific substrate used to assay protein, alkaline and acid phosphatases.
- Can be used for quick analysis of the protein phosphatase activity under any conditions
- Advantageous to a radioactive assay because the substrate concentration can be much higher than the Km
- Activity is measured using a continuous or single-point spectrophotometric assay based on the ability of phosphatases to catalyze the hydrolysis of PNPP to p-nitrophenol, a chromogenic product that absorbs maximally at 405 nm