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pCLIPf Vector
pCLIPf Vector is a mammalian expression plasmid intended for the cloning and stable or transient expression of CLIP-tag® protein fusions in mammalian cells. This plasmid encodes CLIPf, a CLIP-tag protein, which is expressed under control of the CMV promoter.
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- The expression vector has an IRES (internal ribosome entry site) and a neomycin resistance gene downstream of the CLIPf for the efficient selection of stable transfectants
- pCLIPf Vector contains two multiple cloning sites to allow cloning of the fusion partner as a fusion to the N- or C-terminus of the CLIPf
- pCLIPf contains an improved version of CLIP-tag
- CLIPf displays faster kinetics in in vitro labeling than CLIP-tag, and fast, specific and efficient labeling in live and fixed cell applications
