EMFRET R300 产品说明书

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德国EMFRET公司专注心血管和血液系统研究用抗体研发和生产,供应的抗体包括清除血液中血小板、流式细胞术鉴定分析小鼠血小板表面糖蛋白,EMFRET公司的抗体不仅适用于流失分析、WB检测,还可用于免疫组化/免疫荧光及免疫共沉淀检测。

 

 

Antibodies for Plaet Depletion in Mice

# R300

 

Product Information

 

Catalog Number:

R300 (lot # M)

Isotype:

Rat IgG (Wistar)

Contents:

0.5 mg purified immunoglobulins in PBS, sterile filtered

Concentration:

0.5 mg/ml

For research use only, not for diagnostic or therapeutic use. This product is no medical device.

Specificity: This antibody preparation contains a mixture of purified rat monoclonal antibodies

directed against mouse GPIba (CD42b). Targeting of this receptor with divalent IgGs results in profound and irreversible Fc-independent plaet depletion in mice1,2. Plaet depletion is frequently used to study the general role of plaets in (patho-) physiology3.

Preparation and Storage: The antibodies were purified from hybridoma cell culture supernatant by Protein G-Sepharose chromatography and contain < 0.00025 ng/µg (< 0.0025 EU/µg) protein endotoxin. Stable for six months from date of shipment when stored at 4°C. KEEP STERILE, the preparation contains no preservative. Aliquots can be stored at -20°C for at least one year. Avoid repeated freezing and thawing.

Usage: This preparation is optimized to reduce the plaet count by >95% within 60 minutes after intravenous injection of 2 µg/g bodyweight. It is recommended to inject the antibodies in a volume of 100-200 µl (diluted with sterile PBS).

Depletion of mouse plaets by anti-GPIba treatment

Wild type mice received 2 or 4 µg/g plaet depletion antibodies in 200 µl sterile PBS intravenously and plaet counts were determined at the indicated time points. Results are mean ± s.d. of 6 mice per group.

  • Nieswandt B, Bergmeier W, Rackebrandt K, Gessner JE, and Zirngibl H. (2000) Identification of critical antigen-specific mechanisms in the development of immune thrombocytopenic purpura in mice. Blood. 96:2520-27.
  • Elzey BD, Tian J, Jensen RJ et al. (2003) Plaet-mediated modulation of adaptive immunity. A communication link between innate and adaptive immune compartments. Immunity.19:9-19.
R300 Antibodies for Mouse Plaet Depletion polyclonal anti-GPIb alpha Rat IgG purif. plaet depletion 0.5 mg

 

其它产品信息如下:

货号

抗原类型

克隆方式

表型

产品形式

应用

规格

C301

清除小鼠血小板

polyclonal non-immune rat immunoglobulins

Rat IgG

纯化

清除小鼠血小板

0.5 mg

D200

双色分析血小板活性

Wug.E9 JON/A

Rat IgG2b/IgG1

FITCPE

流式细胞术

2 x 1.0 ml; 200 tests

M011-0

GPVI

JAQ1

Rat IgG2a

纯化

IP, IHC, WB

0.1 mg

M011-1

GPVI

JAQ1

Rat IgG2a

FITC

FC, IHC

1.5 ml; 300 tests

M021-0

Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61)

Leo.H4

Rat IgG2b

purif.

IP, IHC, block

0.5 mg

M021-1

Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61)

Leo.H4

Rat IgG2b

FITC

FC, IHC

1.5 ml; 300 tests

M022-0

Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61)

Leo.A1

Rat IgG1

purif.

IP, IHC

0.5 mg

M023-2

Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61)

JON/A

Rat IgG2b

PE

FC (activ. form)

1.5 ml; 300 tests

M024-0

Integrin alphaIIb (GPIIb, CD41)

MwReg30

Rat IgG1

purif.

IP, IHC, generation of Fab fragments

6 mg

M025-0

Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61)

Leo.F2

Rat IgG2a

purif.

IP, IHC

0.5 mg

M025-1

Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61)

Leo.F2

Rat IgG2a

FITC

FC, IHC

1.5 ml; 300 tests

M025-2

Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61)

Leo.F2

Rat IgG2a

PE

FC, IHC

1.5 ml; 300 tests

M025-3

Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61)

Leo.F2

Rat IgG2a

DyLight 649

FC, IHC

1.5 ml; 300 tests

M030-0

Integrin beta3 chain (GPIIIa, CD61)

Luc.A5

Rat IgG2a

purif.

IP, IHC, WB

0.5 mg

M031-0

Integrin beta3 chain (GPIIIa, CD61)

Luc.H11

Rat IgG2b

purif.

IP, IHC, WB

0.5 mg

M031-1

Integrin beta3 chain (GPIIIa, CD61)

Luc.H11

Rat IgG2b

FITC

FC, IHC

1.5 ml; 300 tests

M040-0

GPIbalpha (CD42b)

Xia.G5

Rat IgG2b

purif.

IP, IHC

0.5 mg

M040-1

GPIbalpha (CD42b)

Xia.G5

Rat IgG2b

FITC

FC, IHC

1.5 ml; 300 tests

M040-2

GPIbalpha (CD42b)

Xia.G5

Rat IgG2b

PE

FC, IHC

1.5 ml; 300 tests

M040-3

GPIbalpha (CD42b)

Xia.G5

Rat IgG2b

DyLight 649

FC, IHC

1.5 ml; 300 tests

M041-0

GPIbalpha (CD42b)

Xia.H10

Rat IgG2a

purif.

FC, IHC

0.5 mg

M041-1

GPIbalpha (CD42b)

Xia.H10

Rat IgG2a

FITC

FC, IHC

1.5 ml; 300 tests

M042-0

GPIbalpha (CD42b)

Xia.G7

Rat IgG1

purif.

IP, IHC, WB

0.5 mg

M042-1

GPIbalpha (CD42b)

Xia.G7

Rat IgG1

FITC

FC, IHC

1.5 ml; 300 tests

M043-0

GPIbalpha (CD42b)

Xia.B2

Rat IgG2a

purif.

IP, IHC, block

0.5 mg

M043-1

GPIbalpha (CD42b)

Xia.B2

Rat IgG2a

FITC

FC, IHC

1.5 ml; 300 tests

M050-0

GPIbbeta (CD42c)

Xia.C3

Rat IgG2a

purif.

IP, IHC, WB

0.5 mg

M050-1

GPIbbeta (CD42c)

Xia.C3

Rat IgG2a

FITC

FC, IHC

1.5 ml; 300 tests

M051-0

GPIX (CD42a)

Xia.B4

Rat IgG1

purif.

IP, IHC

0.5 mg

M051-1

GPIX (CD42a)

Xia.B4

Rat IgG1

FITC

FC, IHC

1.5 ml; 300 tests

M060-1

GPV (CD42d)

Gon.C2

Rat IgG1

FITC

FC, IHC

1.5 ml; 300 tests

M061-1

GPV (CD42d)

Gon.G6

Rat IgG2a

FITC

FC, IHC

1.5 ml; 300 tests

M070-0

Integrin alpha2 chain (CD49b, GPIa)

Sam.G4

Rat IgG2b

purif.

IP, IHC, block

0.5 mg

M070-1

Integrin alpha2 chain (CD49b, GPIa)

Sam.G4

Rat IgG2b

FITC

FC, IHC

1.5 ml; 300 tests

M071-0

Integrin alpha2 chain (CD49b, GPIa)

Sam.C1

Rat IgG2b

purif.

IP, IHC

0.5 mg

M071-1

Integrin alpha2 chain (CD49b, GPIa)

Sam.C1

Rat IgG2b

FITC

FC, IHC

1.5 ml; 300 tests

M075-0

Integrin alpha2 chain (CD49b, GPIa)

Sam.C1, Sam.G4, Sam.H9

Rat IgG2b/IgG1

purif.

IP, IHC

0.5 mg

M080-0

Integrin alpha5 chain (CD49e)

Tap.A12

Rat IgG1

purif.

IP, IHC

0.5 mg

M080-1

Integrin alpha5 chain (CD49e)

Tap.A12

Rat IgG1

FITC

FC, IHC

1.5 ml; 300 tests

M110-0

CD9

Nyn.H3

Rat IgG2a

purif.

IP, IHC, WB

0.5 mg

M110-1

CD9

Nyn.H3

Rat IgG2a

FITC

FC, IHC

1.5 ml; 300 tests

M120-0

CD31 (PECAM-1)

Pec.H3

Rat IgG1

purif.

IP, IHC, WB

0.5 mg

M130-0

CD62P (P-selectin)

Wug.E9

Rat IgG1

purif.

IP, IHC

0.5 mg

M130-1

CD62P (P-selectin)

Wug.E9

Rat IgG1

FITC

FC, IHC

1.5 ml; 300 tests

M130-2

CD62P (P-selectin)

Wug.E9

Rat IgG1

PE

FC, IHC

1.5 ml; 300 tests

P140-1

fibrinogen

polyclonal

Rabbit IgG

FITC

FC, IHC

1.5 ml; 300 tests

P150-1

von Willebrand Factor (vWF)

polyclonal

Rabbit IgG

FITC

FC, IHC

1.5 ml; 300 tests

P180-1

negative control

polyclonal

Rabbit IgG

FITC

FC, IHC

1.0 ml; 200 tests

P190-1

negative control

polyclonal

Rat IgG

FITC

FC, IHC

1.0 ml; 200 tests

P190-2

negative control

polyclonal

Rat IgG

PE

FC, IHC

1.0 ml; 200 tests

 

P190-3

negative control

polyclonal

Rat IgG

DyLight 649

FC, IHC

1.0 ml; 200 tests

R300

Antibodies for Mouse Plaet Depletion

polyclonal anti-GPIb alpha

Rat IgG

purif.

plaet depletion

0.5 mg

X488

Antibodies for In Vivo Mouse Plaet Labeling

anti – GPIbbeta derivative

Rat IgG

DyLight 488

in vivo

100 µg

X649

Antibodies for In Vivo Mouse Plaet Labeling

anti – GPIbbeta derivative

Rat IgG

DyLight 649

in vivo

100 µg

 

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