Glen Research 10-1001说明书


美国GRC公司:Glen Research Corp 创建于1987年,专注于为DNA和RNA寡核苷酸的化学合成,修饰,标记和纯化提供zui广泛的亚磷酰胺和固体支持物,现已有近三十年的历史,在低聚核苷酸及修饰、改性领域一直处于地位。

结构研究

以下产品用于研究当关键结构元件改变时对寡核苷酸活性的影响。7-脱氮嘌呤单体缺乏对氢键合至关重要的基团。7-Deaza-8-aza-A和7-deaza-8-aza-G(PPG)单体是A和G的异构体,并且具有相似的电子密度。它们在寡核苷酸中的存在相对于A和G略微稳定。与G不同,PPG不会导致聚集,并且可以容易地制备和分离富含G的寡核苷酸。在5'-末端具有PPG的5'-荧光素寡核苷酸比等同的G寡核苷酸淬灭得少得多。作为胸苷的嘌呤类似物,7-脱氮-2'-脱氧黄嘌呤核苷(7-deaza-dX)有望对三链体的DNA结构产生有趣的影响。7-Deaza-dX还与2,4-二氨基嘧啶核苷类似物形成非标准碱基对。标准核碱基具有未共享的电子对,其突出到双链DNA的小沟中。与DNA,聚合酶,逆转录酶,限制酶等相互作用的酶可以使用氢键供体基团与小沟中的氢键受体接触。3-Deaza-2'-deoxyadenosine非常有趣,因为它保持了常规Watson-Crick氢与T键合的能力,但缺乏通常由N3提供的3-位的电子对。

 

 

 

GlenResearch 目录号:10-1001-xx 

7-Deaza-dA-CE Phosphoramidite
描述:7- Deaza -dA-CE亚磷酰胺

 

5'-二甲氧基三苯甲基-N-苯甲酰基-7-脱氮-2'-脱氧腺苷,3' – [(2-氰基乙基) – (N,N-二异丙基)] – 亚磷酰胺

分子式:C 4 8 H 5 3 N 6 O 7 P. 

MW:856.96 

FW:312.22 

 

稀释剂:无水乙腈

耦合:合成器制造商推荐的标准方法无需更改。

去保护:合成器制造商推荐的标准方法无需更改。

储存:冷藏,高2-8°C,干燥

溶液中的稳定性:类似于dA,C,G,T-CE亚磷酰胺

   

 

10-1001-95

50µm

10-1001-90

100µm

10-1001-02

0.25g

EXTINCTION DATA

Item

Nucleoside

λMax-1

Emax-1

λMax-2

Emax-2

E260

 

 

(nm)

(ml/µmole)

nm

(ml/µmole)

(ml/µmole)

10-1001

7-deaza-dA

270

11.3

 

 

9.4

DILUTION/COUPLING DATA

DILUTION/COUPLING DATA

 

下表显示了包装尺寸数据,以及基于正常灌注程序的溶液,稀释液和近似偶联。请与各种合成器链接以获取更详细的使用信息

 

ABI 392/394

Cat.No.

Pack
Size

Grams/
Pack

0.1M Dil.
(mL)

LV40

LV200

40nm

0.2µm

1µm

10µm

Approximate Number of Additions

10-1001-02

0.25grams

.25grams

2.92

84

50.4

31.5

22.91

16.8

4.2

10-1001-90

100µmoles

.086grams

1

20

12

7.5

5.45

4

1

10-1001-95

50µmoles

.043grams

.5

3.33

2

1.25

.91

.67

.17

Expedite

Cat.No.

Pack
Size

Grams/
Pack

Dilution
(mL)

Molarity

50nm

0.2µm

1µm

15µm

 

Approximate Number of Additions

10-1001-02

0.25grams

.25grams

4.35

.07

80.6

50.38

36.64

5.04

 

10-1001-90

100µmoles

.086grams

1.5

.07

23.6

14.75

10.73

1.48

 

10-1001-95

50µmoles

.043grams

.75

.07

8.6

5.38

3.91

.54

 

 

 

 

货号

品名

规格

目录价

品牌

10-0001-02

dA-5'-CE Phosphoramidite

0.25grams

1275

glen research

10-0001-05

dA-5'-CE Phosphoramidite

0.5grams

2550

glen research

10-0001-10

dA-5'-CE Phosphoramidite

1.0gram

5100

glen research

10-0101-02

dC-5'-CE Phosphoramidite

0.25grams

1275

glen research

10-0101-05

dC-5'-CE Phosphoramidite

0.5grams

2550

glen research

10-0101-10

dC-5'-CE Phosphoramidite

1.0gram

5100

glen research

10-0301-02

dT-5'-CE Phosphoramidite

0.25grams

1275

glen research

10-0301-05

dT-5'-CE Phosphoramidite

0.5grams

2550

glen research

10-0301-10

dT-5'-CE Phosphoramidite

1.0gram

5100

glen research

10-1000-02

dA-CE Phosphoramidite

0.25grams

212.5

glen research

10-1000-02M

dA-CE Phosphoramidite

0.25grams

212.5

glen research

10-1000-05

dA-CE Phosphoramidite

0.5grams

425

glen research

10-1000-05M

dA-CE Phosphoramidite

0.5grams

425

glen research

10-1000-10

dA-CE Phosphoramidite

1.0gram

850

glen research

10-1000-10M

dA-CE Phosphoramidite

1.0gram

850

glen research

10-1000-1C

dA-CE Phosphoramidite

1.0gram

850

glen research

10-1000-1S

dA-CE Phosphoramidite

10.0grams

8500

glen research

10-1000-20

dA-CE Phosphoramidite

2.0grams

1700

glen research

10-1000-2C

dA-CE Phosphoramidite

2.0grams

1700

glen research

10-1000-40

dA-CE Phosphoramidite

4.0grams

3400

glen research

10-1000-50

dA-CE Phosphoramidite

5.0grams

4250

glen research

10-1000-5S

dA-CE Phosphoramidite

5.0grams

4250

glen research

10-1000-C2

dA-CE Phosphoramidite

0.25grams

212.5

glen research

10-1000-C5

dA-CE Phosphoramidite

0.5grams

425

glen research

10-1001-02

7-Deaza-dA-CE Phosphoramidite

0.25grams

16575

glen research

10-1001-90

7-Deaza-dA-CE Phosphoramidite

100µmoles

6035

glen research

10-1001-95

7-Deaza-dA-CE Phosphoramidite

50µmoles

3017.5

glen research

10-1002-02

dA+dG-CE Phosphoramidite

0.25grams

680

glen research

10-1003-02

N6-Me-dA-CE Phosphoramidite

0.25grams

8415

glen research

10-1003-90

N6-Me-dA-CE Phosphoramidite

100µmoles

2762.5

glen research

10-1004-02

3'-dA-CE Phosphoramidite

0.25grams

16575

glen research

10-1004-90

3'-dA-CE Phosphoramidite

100µmoles

6035

glen research

10-1004-95

3'-dA-CE Phosphoramidite

50µmoles

3017.5

glen research

10-1006-02

Etheno-dA-CE Phosphoramidite

0.25grams

4335

glen research

10-1006-90

Etheno-dA-CE Phosphoramidite

100µmoles

1785

glen research

10-1007-02

8-Br-dA-CE Phosphoramidite

0.25grams

5015

glen research

10-1007-90

8-Br-dA-CE Phosphoramidite

100µmoles

1955

glen research

10-1008-02

8-oxo-dA-CE Phosphoramidite

0.25grams

6035

glen research

10-1008-90

8-oxo-dA-CE Phosphoramidite

100µmoles

2295

glen research

10-1010-02

dC-CE Phosphoramidite

0.25grams

212.5

glen research

10-1010-02M

dC-CE Phosphoramidite

0.25grams

212.5

glen research

10-1010-05

dC-CE Phosphoramidite

0.5grams

425

glen research

10-1010-05M

dC-CE Phosphoramidite

0.5grams

425

glen research

10-1010-10

dC-CE Phosphoramidite

1.0gram

850

glen research

10-1010-10M

dC-CE Phosphoramidite

1.0gram

850

glen research

10-1010-1C

dC-CE Phosphoramidite

1.0gram

850

glen research

10-1010-1S

dC-CE Phosphoramidite

10.0grams

8500

glen research

10-1010-20

dC-CE Phosphoramidite

2.0grams

1700

glen research

10-1010-2C

dC-CE Phosphoramidite

2.0grams

1700

glen research

10-1010-40

dC-CE Phosphoramidite

4.0grams

3400

glen research

10-1010-50

dC-CE Phosphoramidite

5.0grams

4250

glen research

10-1010-5S

dC-CE Phosphoramidite

5.0grams

4250

glen research

10-1010-C2

dC-CE Phosphoramidite

0.25grams

212.5

glen research

10-1010-C5

dC-CE Phosphoramidite

0.5grams

425

glen research

10-1013-02

dC+dT-CE Phosphoramidite

0.25grams

680

glen research

10-1014-02

pdC-CE Phosphoramidite

0.25grams

4165

glen research

10-1014-05

pdC-CE Phosphoramidite

0.5grams

8330

glen research

10-1014-90

pdC-CE Phosphoramidite

100µmoles

1445

glen research

10-1015-02

Ac-dC-CE Phosphoramidite

0.25grams

212.5

glen research

10-1015-02M

Ac-dC-CE Phosphoramidite

0.25grams

212.5

glen research

10-1015-05

Ac-dC-CE Phosphoramidite

0.5grams

425

glen research

10-1015-05M

Ac-dC-CE Phosphoramidite

0.5grams

425

glen research

10-1015-10

Ac-dC-CE Phosphoramidite

1.0gram

850

glen research

10-1015-10M

Ac-dC-CE Phosphoramidite

1.0gram

850

glen research

10-1015-1C

Ac-dC-CE Phosphoramidite

1.0gram

850

glen research

10-1015-1S

Ac-dC-CE Phosphoramidite

10.0grams

8500

glen research

10-1015-20

Ac-dC-CE Phosphoramidite

2.0grams

1700

glen research

10-1015-2C

Ac-dC-CE Phosphoramidite

2.0grams

1700

glen research

10-1015-40

Ac-dC-CE Phosphoramidite

4.0grams

3400

glen research

10-1015-50

Ac-dC-CE Phosphoramidite

5.0grams

4250

glen research

10-1015-5S

Ac-dC-CE Phosphoramidite

5.0grams

4250

glen research

10-1015-C2

Ac-dC-CE Phosphoramidite

0.25grams

212.5

glen research

10-1015-C5

Ac-dC-CE Phosphoramidite

0.5grams

425

glen research

10-1016-02

TMP-F-dU-CE Phosphoramidite

0.25grams

8415

glen research

10-1016-90

TMP-F-dU-CE Phosphoramidite

100µmoles

3315

glen research

10-1017-02

Pyrrolo-dC-CE Phosphoramidite

0.25grams

11475

glen research

10-1017-90

Pyrrolo-dC-CE Phosphoramidite

100µmoles

3740

glen research

10-1017-95

Pyrrolo-dC-CE Phosphoramidite

50µmoles

1870

glen research

10-1018-02

5-Me-dC Brancher Phosphoramidite

0.25grams

8585

glen research

10-1018-90

5-Me-dC Brancher Phosphoramidite

100µmoles

3485

glen research

10-1019-02

Amino-Modifier C6 dC

0.25grams

7650

glen research

10-1019-90

Amino-Modifier C6 dC

100µmoles

3825

glen research

10-1020-02

dG-CE Phosphoramidite

0.25grams

212.5

glen research

10-1020-02M

dG-CE Phosphoramidite

0.25grams

212.5

glen research

10-1020-05

dG-CE Phosphoramidite

0.5grams

425

glen research

10-1020-05M

dG-CE Phosphoramidite

0.5grams

425

glen research

10-1020-10

dG-CE Phosphoramidite

1.0gram

850

glen research

10-1020-10M

dG-CE Phosphoramidite

1.0gram

850

glen research

10-1020-1C

dG-CE Phosphoramidite

1.0gram

850

glen research

10-1020-1S

dG-CE Phosphoramidite

10.0grams

8500

glen research

10-1020-20

dG-CE Phosphoramidite

2.0grams

1700

glen research

10-1020-2C

dG-CE Phosphoramidite

2.0grams

1700

glen research

10-1020-40

dG-CE Phosphoramidite

4.0grams

3400

glen research

10-1020-50

dG-CE Phosphoramidite

5.0grams

4250

glen research

10-1020-5S

dG-CE Phosphoramidite

5.0grams

4250

glen research

10-1020-C2

dG-CE Phosphoramidite

0.25grams

212.5

glen research

10-1020-C5

dG-CE Phosphoramidite

0.5grams

425

glen research

10-1021-02

7-deaza-dG-CE Phosphoramidite

0.25grams

16575

glen research

10-1021-90

7-deaza-dG-CE Phosphoramidite

100µmoles

6035

glen research

10-1021-95

7-deaza-dG-CE Phosphoramidite

50µmoles

3017.5

glen research

10-1023

dA+dC+dG+dT-CE Phosphoramidite Mix

1.0gram

680

glen research

10-1027-02

8-Br-dG-CE Phosphoramidite

0.25grams

4335

glen research

10-1027-90

8-Br-dG-CE Phosphoramidite

100µmoles

1785

glen research

10-1028-02

8-oxo-dG-CE Phosphoramidite

0.25grams

16575

glen research

10-1028-90

8-oxo-dG-CE Phosphoramidite

100µmoles

6035

glen research

10-1028-95

8-oxo-dG-CE Phosphoramidite

50µmoles

3017.5

glen research

10-1029-02

dmf-dG-CE Phosphoramidite

0.25grams

212.5

glen research

10-1029-02M

dmf-dG-CE Phosphoramidite

0.25grams

212.5

glen research

10-1029-05

dmf-dG-CE Phosphoramidite

0.5grams

425

glen research

10-1029-05M

dmf-dG-CE Phosphoramidite

0.5grams

425

glen research

10-1029-10

dmf-dG-CE Phosphoramidite

1.0gram

850

glen research

10-1029-10M

dmf-dG-CE Phosphoramidite

1.0gram

850

glen research

10-1029-1C

dmf-dG-CE Phosphoramidite

1.0gram

850

glen research

10-1029-1S

dmf-dG-CE Phosphoramidite

10.0grams

8500

glen research

10-1029-20

dmf-dG-CE Phosphoramidite

2.0grams

1700

glen research

10-1029-2C

dmf-dG-CE Phosphoramidite

2.0grams

1700

glen research

10-1029-40

dmf-dG-CE Phosphoramidite

4.0grams

3400

glen research

10-1029-50

dmf-dG-CE Phosphoramidite

5.0grams

4250

glen research

10-1029-5S

dmf-dG-CE Phosphoramidite

5.0grams

4250

glen research

10-1029-C2

dmf-dG-CE Phosphoramidite

0.25grams

212.5

glen research

10-1029-C5

dmf-dG-CE Phosphoramidite

0.5grams

425

glen research

10-1030-02

dT-CE Phosphoramidite

0.25grams

212.5

glen research

10-1030-02M

dT-CE Phosphoramidite

0.25grams

212.5

glen research

10-1030-05

dT-CE Phosphoramidite

0.5grams

425

glen research

10-1030-05M

dT-CE Phosphoramidite

0.5grams

425

glen research

10-1030-10

dT-CE Phosphoramidite

1.0gram

850

glen research

10-1030-10M

dT-CE Phosphoramidite

1.0gram

850

glen research

10-1030-1C

dT-CE Phosphoramidite

1.0gram

850

glen research

10-1030-1S

dT-CE Phosphoramidite

10.0grams

8500

glen research

10-1030-20

dT-CE Phosphoramidite

2.0grams

1700

glen research

10-1030-2C

dT-CE Phosphoramidite

2.0grams

1700

glen research

10-1030-40

dT-CE Phosphoramidite

4.0grams

3400

glen research

10-1030-50

dT-CE Phosphoramidite

5.0grams

4250

glen research

10-1030-5S

dT-CE Phosphoramidite

5.0grams

4250

glen research

10-1030-C2

dT-CE Phosphoramidite

0.25grams

212.5

glen research

10-1030-C5

dT-CE Phosphoramidite

0.5grams

425

glen research

10-1031-02

5'-OMe-dT-CE Phosphoramidite

0.25grams

6035

glen research

10-1031-90

5'-OMe-dT-CE Phosphoramidite

100µmoles

2295

glen research

10-1032-02

O4-Me-dT-CE Phosphoramidite

0.25grams

6035

glen research

10-1032-90

O4-Me-dT-CE Phosphoramidite

100µmoles

2295

glen research

10-1034-02

4-Thio-dT-CE Phosphoramidite

0.25grams

11475

glen research

10-1034-90

4-Thio-dT-CE Phosphoramidite

100µmoles

5015

glen research

10-1034-95

4-Thio-dT-CE Phosphoramidite

50µmoles

2805

glen research

10-1035-02

Carboxy-dT

0.25grams

6120

glen research

10-1035-90

Carboxy-dT

100µmoles

3060

glen research

10-1036-02

2-Thio-dT-CE Phosphoramidite

0.25grams

11475

glen research

10-1036-90

2-Thio-dT-CE Phosphoramidite

100µmoles

5015

glen research

10-1036-95

2-Thio-dT-CE Phosphoramidite

50µmoles

2805

glen research

10-1037-02

Amino-Modifier C2 dT

0.25grams

6120

glen research

10-1037-05

Amino-Modifier C2 dT

0.5grams

12240

glen research

10-1037-90

Amino-Modifier C2 dT

100µmoles

3060

glen research

10-1038-02

Biotin-dT

0.25grams

10625

glen research

10-1038-90

Biotin-dT

100µmoles

5525

glen research

10-1038-95

Biotin-dT

50µmoles

2847.5

glen research

10-1039-02

Amino-Modifier C6 dT

0.25grams

6120

glen research

10-1039-05

Amino-Modifier C6 dT

0.5grams

12240

glen research

10-1039-90

Amino-Modifier C6 dT

100µmoles

3060

glen research

10-1040-02

dI-CE Phosphoramidite

0.25grams

2040

glen research

10-1040-90

dI-CE Phosphoramidite

100µmoles

850

glen research

10-1041-02

2'-DeoxyNebularine-CE Phosphoramidite (Purine)

0.25grams

4335

glen research

10-1041-90

2'-DeoxyNebularine-CE Phosphoramidite (Purine)

100µmoles

1785

glen research

10-1042-02

O6-Phenyl-dI-CE Phosphoramidite

0.25grams

6035

glen research

10-1042-90

O6-Phenyl-dI-CE Phosphoramidite

100µmoles

2295

glen research

10-1044-02

5-Nitroindole-CE Phosphoramidite

0.25grams

5525

glen research

10-1044-90

5-Nitroindole-CE Phosphoramidite

100µmoles

2125

glen research

10-1046-02

2-Aminopurine-CE Phosphoramidite

0.25grams

6035

glen research

10-1046-90

2-Aminopurine-CE Phosphoramidite

100µmoles

2295

glen research

10-1047-02

dP-CE Phosphoramidite

0.25grams

10115

glen research

10-1047-90

dP-CE Phosphoramidite

100µmoles

3315

glen research

10-1048-02

dK-CE Phosphoramidite

0.25grams

10115

glen research

10-1048-90

dK-CE Phosphoramidite

100µmoles

3315

glen research

10-1049-02

dP+dK-CE Phosphoramidite

0.25grams

10115

glen research

10-1049-90

dP+dK-CE Phosphoramidite

100µmoles

3315

glen research

10-1050-02

dU-CE Phosphoramidite

0.25grams

1700

glen research

10-1050-90

dU-CE Phosphoramidite

100µmoles

595

glen research

10-1051-02

O4-Triazolyl-dU-CE Phosphoramidite

0.25grams

6035

glen research

10-1051-90

O4-Triazolyl-dU-CE Phosphoramidite

100µmoles

2295

glen research

10-1052-02

4-Thio-dU-CE Phosphoramidite

0.25grams

11475

glen research

10-1052-90

4-Thio-dU-CE Phosphoramidite

100µmoles

5015

glen research

10-1052-95

4-Thio-dU-CE Phosphoramidite

50µmoles

2805

glen research

10-1053-02

5-OH-dU-CE Phosphoramidite

0.25grams

11475

glen research

10-1053-90

5-OH-dU-CE Phosphoramidite

100µmoles

3825

glen research

10-1054-02

pdU-CE Phosphoramidite

0.25grams

3315

glen research

10-1054-05

pdU-CE Phosphoramidite

0.5grams

6630

glen research

10-1054-90

pdU-CE Phosphoramidite

100µmoles

1105

glen research

10-1055-02

2'-deoxypseudoU-CE Phosphoramidite

0.25grams

16575

glen research

10-1055-90

2'-deoxypseudoU-CE Phosphoramidite

100µmoles

6035

glen research

10-1055-95

2'-deoxypseudoU-CE Phosphoramidite

50µmoles

3017.5

glen research

10-1056-02

Fluorescein-dT Phosphoramidite

0.25grams

11475

glen research

            

 

 

 

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